Phenotypic Assays

Assays that quantify disease phenotype changes in cells require no a priori knowledge of disease mechanism of action. A good example of this is in the field of oncology, where uncontrolled cell proliferation is a hallmark of cancer and can be used as the basis for a phenotypic assay. Cytation and Lionheart can quantify many types of phenotype change, such as counting cells by either fluorescence or brightfield microscopy in the case of cell proliferation. Many other phenotypes can be quantified based on the use of specific fluorescent probes, such as those for oxidative stress, hypoxia, apoptosis, translocation, etc and the image analysis capabilities of Gen5 Image Prime that provide dual masking capabilities and sub-population analyses based on a wide range of calculated metrics.

 MDA-MB-231-cells-at-20x-0-Hours
MDA-MB-231-cells-at-20x-6-Hours
MDA-MB-231-cells-at-20x-24-Hours

Phase contrast monitoring of MDA-MB-231 cells undergoing oridonin-induced apoptosis at 0, 6 and 20 hrs.

 

Application Notes:

 

Visual Abstracts (.mp4):

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.

Autophagy Analysis Using Object Spot Counting
Autophagy Analysis Using Object Spot Counting

Sub-Micron Spot Quantitation of Centromeres
Sub-Micron Spot Quantitation of Centromeres

Plaque Assay
Plaque Assay

Mitochondrial Membrane Potential Analysis Using Object Spot Counting
Mitochondrial Membrane Potential Analysis Using Object Spot Counting

 


 

 

Related Webinars:

 

Related Products:

 

Learn more about the broad range of phenotypic assay applications in our Resources page.

 

Get more info

 

link