Phenotypic Assays

Phenotypic assays are quantitative assays of cellular structure and function using digital widefield microscopy using both fixed and live cells under environment control. They are routinely used to assess whether a compound or drug produces a desired effect, independent of any defined target. To measure phenotypic assays, Gen5 can analyze multiple cellular parameters simultaneously including size, shape, area, and intensity. This provides a comprehensive picture of individual cell phenotypes and entire population phenotypes that allows a researcher to make an educated decision. Assays of this type typically use culture inserts and 6- to 384-well microplates.

Phase contrast monitoring of MDA-MB-231 cells undergoing oridonin-induced apoptosis at 0, 6 and 20 hrs.

Application Notes:

• Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy
• Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes
• Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging

Learn more about the broad range of phenotypic assay applications in our Resources page.