Nucleic Acid Quantification
The quantification of nucleic acids is common to many applications in basic science and clinical research. These assays use either absorbance or fluorescence to measure the concentration of DNA or RNA in samples. Regardless of the method employed, many laboratories have adapted single cuvette protocols to a 96- and 384-well microplate-based format. These standardized formats, with instrumentation capable of recording measurements from them, allow for the rapid quantification of large numbers of samples.
Nucleic acid quantification encompasses key methods including:
- Spectrophotometric determination of dsDNA, ssDNA, RNA at A260
- Fluorometric determination of dsDNA with fluorescent dyes, for example, PicoGreen
- Determination of purity based on A260/A280 ratios
- Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning
- Low-Volume High Throughput Workflow for Analysis of Nucleic Acid Samples for Biobanking
- Fluorometric Quantitation of dsDNA using PicoGreen®
Many more application notes and other technical documentation about nucleic acid quantification are available in resources.
- Agilent BioTek Cytation Hybrid Multi-Mode Reader
- Agilent BioTek Epoch Microplate Spectrophotometer
- Agilent BioTek Epoch2 Microplate Spectrophotometer
- Agilent BioTek Synergy Neo2 Multi-Mode Reader
- Agilent BioTek Synergy H1 Multi-Mode Reader
- Agilent BioTek Synergy HTX Multi-Mode Reader
- Agilent BioTek Synergy LX Multi-Mode Reader
- Agilent BioTek Take3 Micro-Volume Plates
For Research Use Only. Not for use in diagnostic procedures.