Microbiology

 

Gram process

The advent of a lens system, in 1671, enabled the visualization of bacteria and protists in scientific study. The microscope remains a ubiquitous tool for the study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications. These applications include, but are not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, and vaccine development. Also investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.

This varied application base requires imaging instruments compatible with many objective magnifications, dry and oil resolving power, multiple imaging channels, and advanced image analysis capabilities. These features are demonstrated on Agilent BioTek imagers using a number of microbial models. In addition to automated microscopy features, Agilent BioTek cell imaging multimode readers increase selection from a broader range of assays available to the field of microbiology. These assays require absorbance, fluorescence, luminescence, fluorescence polarization, time resolved fluorescence, and Alpha detection capabilities. Other options, such as microfluidic perfusion control, are available with some Agilent BioTek Cytation and Agilent BioTek Lionheart configurations. The options provide even more versatility to increase choice, throughput, and design efficacy in microbiology assay workflows.

The Agilent BioTek LogPhase 600 microbiology reader is purpose-built for running microbial growth assays in up to 4 plates at a time. This capability delivers higher throughput than any other microplate reader currently available. It has the robust shaking and temperature control required for consistent growth, which leads to consistent data.  

 

LogPhase

 

 

Visual Abstracts:

 

Agilent BioTek visual abstracts are brief animated presentations describing the workflow of a specific application.

 

Characterizing Yeast Vitality
Multi-Mode Metabolic Profiling of Live Yeast Cells

 

Counting Bacilli in Agar Mounted Soil Samples
Counting Bacilli in Agar Mounted Soil Samples

 

Quantitative Multiplexed of Mixed Bacteria
Quantitative Multiplexed of Mixed Bacteria

Gram Stain Imaging
Gram Stain Imaging

Direct Fluorescence Assay (DFA)
Direct Fluorescence Assay (DFA)

 

Acid Fast Bacteria (AFB) Scoring
Acid Fast Bacteria (AFB) Scoring

 

Automatic Transfection Efficiency Calculation in Microplates
Automatic Transfection Efficiency Calculation in Microplates

 

Phagocytosis Assay
Phagocytosis Assay

 

Plaque Assay
Plaque Assay

 

Plaque Assay
Automated Analysis of Neutrophil Netosis

 

Characterizing Yeast Vitality
Characterizing Yeast Vitality

 

Application Notes:

 

Infographic:  How to Calculate Cell Count/Volume Using Automated Label Free Image Analysis


How to Calculate Cell Count/Volume Using Automated Label Free Image Analysis

 

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Related Products:

 

Learn more about microbiology applications - search our application notes and technical documentation in resources.

 

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For Research Use Only. Not for use in diagnostic procedures.

RA44216.4014814815
 

 

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