T Cell Activation and Proliferation
T cell activation is an essential initial step of a targeted immune response. Direct and indirect activation of T cells in vitro causes increased cell proliferation and formation of cell aggregates, which can be tracked and quantitated using label-free and fluorescence imaging methods and analysis.
T Cell Activation and Proliferation Assay Procedure
T cells are added to microplate in media containing activating molecules (e.g. anti-CD3 and IL-2).
T Cell Proliferation
T cell expansion results in increased confluence and aggregation detected by Gen5 image analysis tools.
Quantification of T cell proliferation is reported in real time, revealing differential activation levels.
Increase assay throughput and performance
BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis
of the T cell Activation and Proliferation Assay.