Phagocytosis Assay

Macrophages and other phagocytes are specialized cells that consume and digest foreign matter or aberrant cells through phagocytosis. The pH-sensitive bioparticles or probes, including pHrodo-labeled Zymosan bioparticles, are a useful tool to study phagocytosis. These studies enable detailed quantitation of phagocytosis when coupled with kinetic live cell imaging and automated analysis.

 

Phagocytosis Assay Procedure

Assay Setup

Step 1. Assay Setup: pH-sensitive probes that fluoresce once in the acidic environment of the phagolysosome are added to cell culture.

 

Automated kinetic fluorescence imaging

Step 2. Automated Kinetic Fluorescence Imaging: Phagocytic activity is revealed by an increase in bioparticle fluorescence over time.

 

Automated-analysis

Step 3. Automated Analysis: Agilent BioTek Gen5 microplate reader and imager software analysis tools automatically identify and quantitate changes in bioparticle fluorescence in real time.

 

Increase assay throughput and performance

Agilent BioTek BioSpa compatible assay performs live cell analysis on up to 8 microplates. The Agilent BioTek BioSpa live cell imaging system enables multiplate kinetic analysis of the phagocytosis assay.
BioSpa
The BioSpa Live Cell Analysis System
 

 

Resources

 

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