Phagocytosis Assay

Macrophages and other phagocytes are specialized cells that consume and digest foreign matter or aberrant cells through phagocytosis. pH-sensitive bioparticles or probes, including pHrodo™-labeled Zymosan bioparticles, are a useful tool to study phagocytosis and enable detailed quantitation of phagocytosis when coupled with kinetic live cell imaging and automated analysis.

 

Phagocytosis Assay Procedure

Assay Setup

Assay Setup
pH-sensitive probes that fluoresce once in the acidic environment of the phagolysosome are added to cell culture.

 

Automated kinetic fluorescence imaging

Automated Kinetic Fluorescence Imaging Phagocytic activity is revealed by an increase in bioparticle fluorescence over time.

 

Automated-analysis

Automated Analysis
Gen5 image analysis tools automatically identify and quantitate changes in bioparticle fluorescence in real time.

 

Increase assay throughput and performance

BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
BioSpa
The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis
of the Phagocytosis Assay

 

Resources

 

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