NETosis

Neutrophils play a key role in the body’s defense against infections. As part of the innate immune system, they employ multiple strategies to degrade and kill microbes, including the release of neutrophil extracellular traps (NETs). NETs are a web-like structure composed of antimicrobial proteins and DNA that are released during a distinct form of programmed neutrophil cell death known as NETosis. In general, NETs allow neutrophils to kill extracellular pathogens while minimizing damage to the host cells, but can also result in an autoimmune response. This process in vivo is not well understood, so in vitro models are important to help clarify the mechanisms and results of this phenomenon.

 

Netosis Assay Procedure

Assay Setup

Assay Setup

Neutrophils preloaded with plasma membrane integrity stain provide an assay to kinetically monitor NETosis by imaging in microplates.

 

Assay Setup

Automated Kinetic Imaging
Kinetic fluorescence imaging of stimulated cells captures release of NETs over time.

 

Assay Setup

Automated Analysis
Treatment-dependent NETosis is quantified using object masking and fluorescence integral.

Increase assay throughput and performance

BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
BioSpa
The BioSpa Live Cell Analysis System
enables multi-plate kinetic
analysis of the NETosis Assay.

Liquid handling automation

BioSpa

The BioTek MultiFlo™ FX or other Liquid Handler automate cell seeding and treatment addition.

 

Resources

 

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