Immune-cell-mediated cytotoxicity plays an essential role in defending the host from intracellular pathogens. Activated effector cells, including T cells, NK, natural killer T cells (NKT) and granulocytes, are capable of killing targeted cells through unique mechanisms and regulatory pathways. Kinetic imaging and analysis of fluorescently labeled target and effector cells provides an effective and convenient method for characterizing cell-mediated cytotoxicity over time in both 2D and 3D cell culture formats.
Cell-Mediated Cytotoxicity Assay Procedure
T Cell Activation
Labeled T cells (red) attack target cells in media containing fluorescent cytotoxicity probe.
T Cell-Induced Cytotoxicity
Kinetic brightfield and fluorescence imaging of cytotoxicity probe (green) reveals T cell-induced cytotoxicity in target cells.
Detailed profiles of necrosis probe fluorescence over time demonstrate T cell-mediated cytotoxicity.
Increase assay throughput and performance
BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis of the
T cell Activation and Proliferation Assay.
- An Image-Based Method to Detect and Quantify T Cell Mediated Cytotoxicity of 2D and 3D Target Cell Models