Cell-Mediated Cytotoxicity

Immune-cell-mediated cytotoxicity plays an essential role in defending the host from intracellular pathogens. Activated effector cells, including T cells, NK, natural killer T cells (NKT) and granulocytes, are capable of killing targeted cells through unique mechanisms and regulatory pathways. Kinetic imaging and analysis of fluorescently labeled target and effector cells provides an effective and convenient method for characterizing cell-mediated cytotoxicity over time in both 2D and 3D cell culture formats.

 

Cell-Mediated Cytotoxicity Assay Procedure

Assay Setup

T Cell Activation
Labeled T cells (red) attack target cells in media containing fluorescent cytotoxicity probe.

 

Automated kinetic fluorescence imaging

T Cell-Induced Cytotoxicity
Kinetic brightfield and fluorescence imaging of cytotoxicity probe (green) reveals T cell-induced cytotoxicity in target cells.

 

Automated-analysis

Automated Analysis
Detailed profiles of necrosis probe fluorescence over time demonstrate T cell-mediated cytotoxicity.

Increase assay throughput and performance

BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
BioSpa
The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis of the
T cell Activation and Proliferation Assay.

Liquid handling automation

BioSpa

The BioTek MultiFlo™ FX or other Liquid Handler can automate seeding cells into the microplate as well as conduct media exchanges.

 

Resources

 

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