Immunofluorescence an essential tool for interpreting subcellular processes in a diverse variety of scientific research. Invariably, it is coupled with fluorescence microscopy, which provides the subcellular spatial resolution. Today, immunofluorescence reagent options range in scope from vast offerings of individual primary antibodies, either fluorescently labeled or unconjugated and designed to work with secondary antibodies to out-of-box multiplexed assay solutions for signaling pathway analysis. The basic IF workflow of labeling proteins with fluorescently conjugated antibodies, usually in conjunction with cell structure stains is typically laborious and benefits from the use of microplate-based labware (6- to 384-well densities) which can be used with automated liquid handing instruments to wash cells and dispense the reagents. Other than microplates, immunofluorescence of cells and tissues uses a wide range of labware including microscope slides, petri dishes and culture inserts.
BioTek has developed a number of application notes describing immunofluorescence using its line of liquid handling, detection, and imaging instrumentation.
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids
- Automated Imaging and Dual-Mask Analysis of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy
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