Genotoxicity refers to the destructive effects of mutagens such as high energy radiation and chemicals on cellular nucleic acids, particularly nuclear DNA. Single cell gel electrophoresis, or the Comet Assay, can be used to probe DNA fracture by mutagens. The g-H2AX immunofluorescence assay can be used to quantify DNA double strand breaks. Each method has its challenges regarding image analysis, but Agilent BioTek Gen5 data analysis software 3.0 has the ability to quantify either the %DNA in the comet tail or comet tail moment. The Gen5 3.0 can also count g-H2AX foci to determine levels of genotoxicity.
- Automated Imaging and Analysis of a Novel Comet Assay to Enable High Throughput Genotoxicity Testing
- Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis
- Automated Imaging and Dual-Mask Spot Counting of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis
Agilent BioTek visual abstracts are brief animated presentations describing the augmented microscopy workflow of a specific application.
- Agilent BioTek Lionheart FX Automated Microscope
- Agilent BioTek Lionheart LX Automated Microscope
- Agilent BioTek BioSpa Live Cell Imaging System
- Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
- Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
- Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader
Learn more about Genotoxicity applications - search our application notes and technical documentation in resources.