Chemotactic Cell Migration and Invasion Assay

The advent of permeable supports has provided a simple in vitro approach to measure chemoattractant-induced cell migration and invasion. The addition of a fluorescence blocking membrane improves the accuracy of the assay, and allows the use of inverted fluorescence microscopy for detailed kinetic analysis. This provides the ability to accurately count the number of cells reaching the bottom of the membrane instead of relying on PMT-based measurements, which are more vulnerable to background fluorescence.

Chemotactic Cell Migration and Invasion Assay Procedure

Assay setup and analysis
FluoroBlok™ inserts, either untreated or containing biomatrix, are loaded with fluorescently labeled cells. Media containing chemoattractant is added to well reservoir below the insert. Migration of cells to the basolateral surface of the permeable support is characterized using kinetic fluorescence imaging and Gen5 image analysis tools.

Results
This image-based method provides detailed profiles of cell migration over time and has considerable advantages over end point assays and methods that rely on measuring total fluorescent signal.

Increase assay throughput and performance

Resources

• Automated, Kinetic Imaging of Cell Migration and Invasion Assays using Corning FluoroBlok™ Permeable Supports
• Automated, Kinetic Imaging of Cell Migration and Invasion Assays using Corning® FluoroBlok™ Inserts

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