Cell Migration/Invasion

Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models. BioTek’s AutoScratch™ Wound Making Tool makes reproducible scratch wounds in 96- and 24-well plates, while Gen5 3.0 software offers numerous methods of automated image analysis that can be used to quantify migration and invasion such as wound width, confluence or healing rate in the case of scratch assays; or invadopodia area for tumor invasion assays incorporating spheroid co-cultures embedded in matrigel.

HCT116 Uninhibited Cell Migration

HT-1080 Uninhibited Wound Healing

AutoScratch Wound Making Tool

Application Notes:

• Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays
• Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells
• Automated, Kinetic Imaging of Cell Migration and Invasion Assays using Corning FluoroBlok™ Permeable Supports
• Imaging and Analysis of a High Density Cell Migration Assay
• Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition

Visual Abstracts:

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.