Cell Metabolism
Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis. BioTek’s imaging products Cytation™ 1 and Cytation 5 can be used to normalize Agilent’s Seahorse OCR and ECAR replicate data; while our microplate readers, Synergy™ H1, Synergy Neo2 and Cytation all containing time-resolved fluorescence modules can be used to read Agilent’s soluble sensor family of products.
Agilent Seahorse XF Imaging and Normalization System
Visual Abstracts:
Multi-Mode Metabolic Profiling of Live Yeast Cells
Application Notes:
- Optimization of a Multi-Mode Detection Model for Measuring Real-Time Cellular Respiration and Mitochondrial Function using Fluorescent Biosensors
- XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System
- Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5
Application Bulletins:
- BioTek Resources for Agilent MitoXpress® Xtra Oxygen Consumption Assay
- BioTek Resources for Agilent MitoXpress® Intra Intracellular Oxygen Assay
- BioTek Resources for Agilent pH-Xtra™ Glycolysis Assay
Technical Notes:
Related Products:
- Cytation 7 Cell Imaging Multi-Mode Reader
- Cytation 5 Cell Imaging Multi-Mode Reader
- Cytation 1 Cell Imaging Multi-Mode Reader
- Synergy Neo2 Hybrid Multi-Mode Reader
- Synergy H1 Hybrid Multi-Mode Reader