Cell Metabolism: Seahorse XF Imaging and Normalization
Agilent Technologies offers an integrated imaging and normalization system which combines cellular analysis and imaging for an intuitive workflow to streamline your XF data interpretation. This imaging and normalization solution combines Agilent's strength in cellular metabolic analysis with BioTek's capability in cell imaging.
Example of XF data normalization using in situ nuclear staining and in situ cell counting.
SKOV3 cells were plated at 1x104, 2x104, 3x104 cells per well, cultured 24 h, subject to the XF Cell Energy Phenotype Test and followed by image analysis. A) Raw OCR and ECAR change with injection (arrows) of oligomycin + FCCP (1.0 µM and 0.5 µM final, respectively), including 20 µM Hoechst 33342 (2 µM final). B) Representative images of nuclei fluorescently labeled by Hoechst 33342 (upper panel) and nuclei identified and outlined using the Seahorse XF Imaging and Cell Counting Software with the Cytation 1 (lower panel). C) OCR and ECAR normalized by in situ nuclear staining cell counts (Mean ± SD, n=4).
Watch this video to see how this solution improves XF data interpretation.
See the normalization workflow in 4 easy steps
Application Notes:
- XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System
- Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5
Technical Notes:
Related Products:
- Cytation 5 Cell Imaging Multi-Mode Reader
- Cytation 1 Cell Imaging Multi-Mode Reader
- Agilent Seahorse XF