Molecular Biology and Biochemical

ELISABiochemical assays run the gamut from routine ELISAs to microvolume nucleic acid and protein quantification. Endpoint and kinetic, single-, and multiwavelength assays are essential experimental tools. The Agilent BioTek multimode readers have the flexibility to accommodate a broad range of applications with superior performance.

 

ELISA

The Enzyme Linked Immunosorbent Assay (ELISA) is a common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. While some of these technologies utilize low volume analysis in 1536-well plates, ELISA performed in 96-well microplates remains the most commonly used immunoassay format. The ELISA technique causes formation of specific immune complexes that are easily measured using Agilent BioTek single or multimode microplate readers.

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Nucleic Acid Quantification

The quantification of nucleic acids is common to many applications in basic science and clinical research. These assays use either absorbance or fluorescence to measure the concentration of DNA or RNA in the sample. Regardless of the method employed, many laboratories have adapted single cuvette protocols to a 96- and 384-well microplate-based format. These standardized formats, with instrumentation capable of recording measurements from them, allow for the rapid quantification of large numbers of samples.

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Total Protein Quantification

Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content.

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Enzyme Activity

 

 

Learn more about Biochemical Assay applications - search our Application Notes and technical documentation in Resources.

 

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For Research Use Only. Not for use in diagnostic procedures.

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