Barrier Cell Migration Assay

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  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
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  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
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Home Applications Cell Migration and Invasion Barrier Cell Migration Assay

Cell exclusion zone assays, or barrier assays, rely on the creation of a cell-free zone without the use of a mechanical “scratch.” Removal of the temporary barrier enables surrounding cells to migrate into the open area. With this application, cell migration into the wound area over time is monitored within the Lionheart™ FX Automated Live Cell Imager or Cytation™ Cell Imaging Multi-mode Reader, with Gen5™ analysis delivering detailed and reproducible cell migration results.

Label-Free Barrier Cell Migration Procedure

Cell Migration Assay: 3D Bioprinting Visual Abstract
Capture
Kinetic high contrast brightfield (HCBF) images our captured based on desired interval and duration.

Analyze
Images are processed and analyzed to monitor migration into the open area.

Cell Migration Assay: 3D Bioprinting Visual Abstract
Report
Kinetic profiles provide detailed information on cell migration across treatment conditions.

Increase assay throughput and performance

BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
BioSpa
The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis
of the Barrier Cell Migration Assay.