Barrier Cell Migration Assay

Cell exclusion zone assays, or barrier assays, rely on the creation of a cell-free zone without the use of a mechanical “scratch.” Removal of the temporary barrier enables surrounding cells to migrate into the open area. With this application, cell migration into the wound area over time is monitored within the  Lionheart™ FX Automated Live Cell Imager or Cytation™ Cell Imaging Multi-mode Reader, with Gen5™ analysis delivering detailed and reproducible cell migration results.

 

Label-Free Barrier Cell Migration Procedure

Cell Migration Assay: 3D Bioprinting Visual Abstract
Capture
Kinetic high contrast brightfield (HCBF) images our captured based on desired interval and duration.

 

Cell Migration Assay: 3D Bioprinting Visual Abstract
Analyze
Images are processed and analyzed to monitor migration into the open area.

 

Cell Migration Assay: 3D Bioprinting Visual Abstract
Report
Kinetic profiles provide detailed information on cell migration across treatment conditions.

 

 

Increase assay throughput and performance

BioSpa™ Compatible Assay: Live cell analysis on up to 8 microplates
BioSpa
The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis
of the Barrier Cell Migration Assay.

Liquid handling automation

 

BioSpa

Integrating the MultiFlo FX Microplate Dispenser automates cell seeding and removes piled-up cells from around cell zone for improved assay results.

 

Application Notes:

 

>> BACK TO CELL MIGRATION AND INVASION

 

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