Automated Culture Techniques for 3D Structures

Culturing cells in 3D provides an environment similar to in vivo conditions, allowing cells to maintain high viability when cultured for extended time periods. To maintain the highest levels of viability within untreated cells and to ensure accurate evaluation of treatment-induced effects, media exchanges and redosing are necessary throughout the experiment. These procedures are essential, particularly in those cultures lasting days to weeks. Media exchanges with cell models that do not rely on adherence to labware can be daunting when relying on manual methods. Multichannel pipettes must remove and dispense media at an extremely slow rate. Also, care must be taken to keep pipette tips away from the actual spheroids to avoid accidentally damaging or removing the spheroids.

The Agilent BioTek MultiFlo FX multimode dispenser with the Agilent BioTek AMX automated media exchange module provides an automated method for effectively performing media exchanges. The exchanges can be done without disturbing unattached 3D spheroids in 96- and 384-well formats. The AMX system delivers more consistent results compared to manual maintenance of 3D cell structures (Figure 1.). When coupled with Agilent BioTek automation, the media exchange tool provides a walk-away solution for long-term 3D experimental procedures.

Brightfield images
Figure 1. Bright-field images of an entire 96-well microplate after one (left image); and three (right image) media exchange cycles. Media exchanges were performed manually in all odd columns, and automated using the MultiFlo FX equipped with the AMX automated media exchange module in all even columns.


Automatic % live/dead calculation
Automated Gentle Media Exchange of Suspension Cells
using the AMX module for the MultiFlo FX



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For Research Use Only. Not for use in diagnostic procedures.