Automated Cell Count and Viability

Assay setup and optimization, as well as routine maintenance of cell cultures, requires accurate determination of cell counts and viability. Manual cell counting with a hemocytometer is time consuming and depends on subjective evaluation by the user, leading to erroneous and inconsistent results.

A range of methods for accurately and efficiently determining cell counts and viability are available for the Agilent BioTek Cytation cell imaging multimode reader and Agilent BioTek Lionheart automated imager. Automated image capture and analysis tools quantify cells loaded into either a reusable glass hemocytometer or disposable counting chambers. Total cell counts are determined using label-free techniques, while trypan blue or fluorescent labels can be used to measure cell viability.


Cell Count and Viability Starter Kit

The Agilent BioTek Cell Count and Viability Starter Kit includes everything a researcher needs to count cells and measure viability in a mammalian cell suspension. The kit is used with the Cytation or the Lionheart system. The kit saves time and increases data quality by automating the tedious and error-prone process of mammalian cell counting.


Whole Organism Imaging
Figure 1. All-inclusive kit: (1) 50 disposable cell counting slides, (2) 4-slide holder, (3) Cell Count & Viability App, (4) quick start guide


Quantifying Poly-Glutamine Aggregates


Cell Count and Viability App Procedure

Cell count determination
Step 1. Cell count determination: Samples are imaged, and total cell counts are automatically determined using the Agilent BioTek Gen5 microplate reader and imager software analysis tools.


Automatic % live/dead calculation
Step 2. Automatic % live/dead calculation: The kit includes trypan blue stain to stain dead cells. Once stained and imaged, the software app automatically calculates the live/dead cell percentage.


Built-in dilution calculator
Step 3. Built-in dilution calculation: The Cell Count and Viability App includes a calculator to automatically determine how much sample and media are required to reach a desired cell concentration, to facilitate downstream applications.

Fluorescence-based Cell Count and Viability

A broad range of imaging LED and filter cubes are available for cell-counting applications involving fluorescent markers designed to measure cell viability. Percent live/dead calculations and subpopulation analysis are performed by Gen5 software based on preset parameters.

Calcein AM
Figure 2. Calcein AM is an esterase substrate that stains live cells green, while ethidium homodimer-III (EthD-III) is a membrane-impermeable DNA dye that stains dead cells red. When these two stains are used in conjunction, they provide a sensitive dual-fluorescence system for conducting automated cell viability measurements.


Application Notes




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For Research Use Only. Not for use in diagnostic procedures.