Automated Cell Count and Viability

Assay setup and optimization, as well as routine maintenance of cell cultures, requires accurate determination of cell counts and viability. Manual cell counting with a hemocytometer is time consuming and depends on subjective evaluation by the user, leading to erroneous and inconsistent results.

A range of methods for accurately and efficiently determining cell counts and viability are available for the Cytation™ Cell Imaging Multi-Mode Reader and Lionheart™ Automated Imager. Automated image capture and analysis tools quantify cells loaded into either a reusable glass hemocytometer or disposable counting chambers. Total cell counts are determined using label-free techniques, while trypan blue or fluorescent labels can be used to measure cell viability.

 

Cell Count and Viability Starter Kit

BioTek’s Cell Count & Viability Starter Kit includes everything a researcher needs to count cells and measure viability in a mammalian cell suspension using a BioTek Cytation Cell Imaging Multi-Mode Reader or a Lionheart Automated Imager. The Kit saves time and increases data quality by automating the tedious and error-prone process of mammalian cell counting.

 

Whole Organism Imaging
All-inclusive kit:  (1) 50 disposable cell counting slides, (2) 4-slide holder, (3) Cell Count & Viability App, (4) quick start guide

 

Quantifying Poly-Glutamine Aggregates
 

 

Cell Count and Viability App Procedure

Cell count determination
Cell count determination
Samples are imaged and total cell counts are automatically determined using Gen5 image analysis tools.

 

Automatic % live/dead calculation
Automatic % live/dead calculation
The kit includes trypan blue stain to stain dead cells. Once stained and imaged, the software app automatically calculates the live/dead cell percentage.

 

Built-in dilution calculator
Built-in dilution calculator
The Cell Count & Viability App includes a calculator to automatically determine how much sample and media are required to reach a desired cell concentration, to facilitate downstream applications.

Fluorescence-based Cell Count and Viability

A broad range of imaging LED and filter cubes are available for cell-counting applications involving fluorescent markers designed to measure cell viability. Percent live / dead calculations and subpopulation analysis are performed by Gen5™ based on preset parameters.

Calcein AM
Calcein AM is an esterase substrate that stains live cells green, while ethidium homodimer-III
(EthD-III) is a membrane-impermeable DNA dye that stains dead cells red. When these two stains
are used in conjunction, they provide a sensitive dual-fluorescence system for conducting
automated cell viability measurements.

 

Application Notes

 

 

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