Kinetic Quantitation of Apoptosis and Necrosis
Apoptosis, or programmed cell death, refers to a series of biochemical changes in the cell leading to distinct morphological changes that can be assessed using fluorescent probes or contrast-enhancing microscopy modes such as phase contrast. Examples of fluorescent probes include those that target phosphatidylserine which becomes exposed to extracellular reagents in early stage apoptosis (i.e. pSIVA-IANBD), fluorometric substrates of or antibodies to caspases (i.e. Caspase 3/7) that generate fluorescence with caspase activation, or probes that measure chromatin condensation. Cytation™ and Lionheart™ FX both have the ability to measure apoptosis through the use of fluorescent probes or by label-free methods relying on phase contrast microscopy.
Application Notes:
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format
- Live Cell Imaging of Apoptosis and Necrosis
- Automated, Multi-Parameter, Kinetic Methods to Quantify Cell Death
- Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity Assay
- Utilizing Automated Imaging and Advanced 3D Cell Culture Techniques to Quantify Apoptosis Activity
- Use of Phase Contrast Imaging to Track Morphological Cellular Changes due to Apoptotic Activity
- Using Acridine Orange to Measure Cell Death In Ethanol Treated Zebrafish Embryos
Visual Abstracts:
BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.
Measuring Apoptosis in Zebrafish
Treated with Ethanol
Live Cell Imaging of Apoptosis and Necrosis
3D Kinetic NK Cell Cytotoxicity Assay
Related Products:
- Lionheart FX Automated Microscope
- Lionheart LX Automated Microscope
- Cytation 7 Cell Imaging Multi-Mode Reader
- Cytation 5 Cell Imaging Multi-Mode Reader
- Cytation 1 Cell Imaging Multi-Mode Reader
Learn more about Kinetic Quantitation of Apoptosis and Necrosis applications - search our Application Notes and technical documentation in Resources.
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