Kinetic Quantitation of Apoptosis and Necrosis

Apoptosis, or programmed cell death, refers to a series of biochemical changes in the cell leading to distinct morphological changes that can be assessed using fluorescent probes or contrast-enhancing microscopy modes such as phase contrast. Examples of fluorescent probes include those that target phosphatidylserine which becomes exposed to extracellular reagents in early stage apoptosis (i.e. pSIVA-IANBD), fluorometric substrates of or antibodies to caspases (i.e. Caspase 3/7) that generate fluorescence with caspase activation, or probes that measure chromatin condensation. Cytation™ and Lionheart™ FX both have the ability to measure apoptosis through the use of fluorescent probes or by label-free methods relying on phase contrast microscopy.

20x Kinetic Apoptosis and Necrosis Movie

 

 

 

 

Application Notes:

• Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format
• Live Cell Imaging of Apoptosis and Necrosis
• Automated, Multi-Parameter, Kinetic Methods to Quantify Cell Death
• Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity Assay
• Utilizing Automated Imaging and Advanced 3D Cell Culture Techniques to Quantify Apoptosis Activity
• Use of Phase Contrast Imaging to Track Morphological Cellular Changes due to Apoptotic Activity
• Using Acridine Orange to Measure Cell Death In Ethanol Treated Zebrafish Embryos

 

Visual Abstracts:

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.

Measuring Apoptosis in Zebrafish
Treated with Ethanol

Live Cell Imaging of Apoptosis and Necrosis

3D Kinetic NK Cell Cytotoxicity Assay

 

Related Products:

• Lionheart FX Automated Microscope
• Lionheart LX Automated Microscope
• Cytation 7 Cell Imaging Multi-Mode Reader
• Cytation 5 Cell Imaging Multi-Mode Reader
• Cytation 1 Cell Imaging Multi-Mode Reader

 

Learn more about Kinetic Quantitation of Apoptosis and Necrosis applications - search our Application Notes and technical documentation in Resources.

 

 

>> BACK TO CELL HEALTH AND VIABILITY

 

Get more info