3D Cell Culture
Over the last decade, a central focus of drug discovery efforts has been the incorporation of in vitro testing models that mimic in vivo conditions found within and around the target. This has led to the development of multiple methods to culture cells in a three-dimensional (3D) format instead of on the bottom of a microplate well in a two-dimensional (2D) format. Some of the most commonly incorporated technologies to create the desired spheroids or tumoroids include, but are not limited to polymeric and biological scaffolds, ultra-low attachment, hanging drop, and magnetic bioprinting.
3D cell culture is now used in diverse application areas such as:
- Drug and Environmental Toxicology
- Stem Cell Research
Many procedures using cells cultured in 3D are performed in a variety of microplate types ranging from 6- to 1536-well format, and are commonly analyzed using microplate reader optics or digital widefield microscopy.
BioTek has developed a suite of application notes describing adaptation of these diverse processes using its line of liquid handling, detection, and imaging instrumentation
- Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells
- Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids
Learn more about 3D Cell Culture applications - search our Application Notes and technical documentation in Resources.