Join us for Insights Fall 2019
Boston Marriott Cambridge
50 Broadway, Cambridge, MA 02142
November 15th, 2019
Insights 2019 at Boston Marriott Cambridge is BioTek's second imaging user group meeting. By connecting local scientists and BioTek, as well as key partners including Agilent Technologies, and HighRes Biosolutions, we aim to drive collaboration and innovation amongst Cytation™ and Lionheart™ imager users within the scientific community.
The day will include presentations from prominent scientists who will share highlights of their current research in which BioTek instrumentation has facilitated or enhanced their research objectives, as well as ‘Tips & Tricks’ from BioTek experts and hands on workstations with team scientists. See below for the full day’s agenda.
|8:00 AM – 9:00 AM||Registration, Coffee & Continental Breakfast|
|9:00 AM – 9:05 AM||Introduction|
|9:05 AM – 9:30 AM||New Products|
|9:30 AM – 10:00 AM||
Speaker 1: Dr. Paul Greer (U. Mass. Medical School), Lionheart FX Automated Microscope
Title: An Unexpected Role for Olfactory Receptors in Alzheimer's Disease
This talk will discuss our work examining the role of MS4A odorant receptors in Alzheimer's Disease (AD). MS4A receptors have been strongly linked to altered AD susceptibility through large scale human genetic studies, but their role in AD pathogenesis is unknown. Using a combination of single cell transcriptional assays, mouse genetics, and cell based imaging assays using the Lionheart microscope we have begun to elucidate the role of these proteins in AD.
|10:00 AM – 10:20 AM||Tips & Tricks: Assay Design & Optimization|
|10:20 AM – 10:40 AM||Tips & Tricks: Primary and Secondary Masking|
|10:40 AM – 11:00 AM||Coffee Break|
|11:00 AM – 11:30 AM||
Speaker 2: Joseph E. Italiano, PhD (Harvard Medical School), Lionheart FX Automated Microscope
Title: Imaging Platelet-Related Phenomena Using Lionheart FX
Platelets are anucleate cells that circulate in the blood and serve to prevent bleeding and minimize blood vessel injury. Our lab is interested in the mechanisms involved in platelet production from their precursor cells, megakaryocytes, and the subsequent role of platelets in various disease states. This research relies heavily on fixed and live cell imaging at the megakaryocyte and platelet level, which will greatly benefit from using the Biotek Lionheart FX microscopy platform in a variety of applications.
|11:30 AM – 12:00 PM||
Speaker 3: Dr. Cameron Myhrvold, Cytation 5 (The Broad Institute)
Title: Programmable Antivirals Using CRISPR-Cas13Anchor
We recently reprogrammed the CRISPR effector protein Cas13 to target and cleave the genomes of three RNA viruses, including influenza virus. We observed a >300-fold knockdown of influenza infectivity, and investigated the effects of target sequence, multiplexing, and other factors on targeting efficiency. All of the data for this paper (aside from qPCR and next-generation sequencing) was gathered using a Cytation 5 plate reader.
|12:00 PM – 1:00 PM||
Lunch, Instrumentation Display
|1:00 PM – 1:30 PM||Agilent Seahorse XFe Applications and Normalization|
|1:30 PM – 1:50 PM||Tips & Tricks: Spot Counting|
|1:50 PM – 2:10 PM||Tips & Tricks: Graph Making, Statistics, and Reporting|
|2:10 PM – 2:40 PM||
Speaker 4: Charlotte Chung (Voyager Therapeutics), Cytation 5 Cell Imaging Multi-Mode Reader
Title: Image Analysis of AAV Transduction in Primary Neuron Co-Culture
Primary neuron co-culture which contains multiple cell types were transduced with AAV and imaged by Cytation 5 and Gen5 image analysis software. Transduction efficiency in each cell type were quantified using Gen5 dual masking.
|2:40 PM – 3:10 PM||
Speaker 5: Hayley Meundlein (Tufts U.), Cytation 5 Cell Imaging Multi-Mode Reader
Title: cFLIPL Protects Macrophages From LPS Induced Pyroptosis and IL-1b Release
Our lab and others have recently identified a novel form of caspase-8-mediated pyroptosis in LPS-activated macrophages. Using the Cytation 3, we have been able to show that this pyroptosis, and concomitant NLRP3 inflammasome activation and IL-1b release are regulated by the caspase-8 homologue cFLIP.
BioTek will feature the following products: