Visit BioTek at AACR 2018
This year, BioTek is celebrating its 50th anniversary! Visit us at Booth 3000 to see how 50 years of passion and innovation have resulted in exciting new instrumentation designed to automate and improve your assay workflows. We'll feature the Lionheart Automated Microscope, MultiFlo FX Dispenser, Agilent’s Seahorse Analyzer with BioTek’s Cytation 1 and much more.
Exhibitor Spotlight Tutorial
Automated, Image-Based Solutions to Assess T Cell Activation and Cell Mediated Cytotoxicity Using 2D and 3D Target Cell Models
Sunday, April 15, 2018 | 3:30 pm - 4:30 pm
Spotlight Theater C
T cell mediated cytotoxicity plays an important role in a suite of new methods being developed with the goal of boosting a patient’s immune system to combat cancer. In order to properly optimize adoptive T cell immunotherapies, accurate, sensitive in vitro methods must first be included prior to clinical testing. In this tutorial, we describe phenotypic and quantitative image-based methods to assess T cell activation, T cell:target cell interactions, and eventual induction of target cell death. Data from 2D and 3D target cell models, as well as T cells activated using general and directed methods (in the presence of target cells) will be presented.
Agilent and BioTek Join Forces to Create an Integrated Metabolic Analysis and Imaging Platform
Featured in BioTek’s booth at AACR 2018 will be a new, integrated solution that combines cellular metabolic analysis and imaging technologies. The solution integrates Agilent’s Seahorse XFe96 and XFe24 Analyzers with BioTek’s Cytation 1 Cell Imaging Multi-Mode Reader. This collaboration creates a standardized approach for comparing XF data sets, improving assay workflow, embedding images into WAVE software and applying normalization values to Seahorse XF measurements.
The Agilent Seahorse XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis—in live cells, in real time. These measurements help scientists to better understand the connection between cellular physiological processes and genomic and proteomic data. The BioTek Cytation 1 Cell Imaging Multi-Mode Reader can be configured for fluorescence and high contrast brightfield cellular imaging with up to 60x magnification and has a wide variety of potential applications.
Agilent’s automated Seahorse XF Analyzer combined with BioTek’s Cytation 1 creates a distinct opportunity for cell biology researchers to fully normalize and analyze samples to answer challenging questions regarding cellular metabolism. For years, Agilent customers have requested an optimized method for verifying assay results and enabling more meaningful data comparisons. This integrated solution gives customers the ability to compare XF data on a well-to-well, plate-to-plate and experiment-to-experiment basis. The ability to incorporate high-quality imagery within Agilent’s Seahorse XF WAVE software adds another dimension to the data. Now researchers can toggle between XF data, brightfield images and fluorescence images in a unified software experience. Referencing the images while analyzing XF data provides evidence and guidance on how to limit variability and improve the reproducibility of their XF assays. Applying a cell count based normalization value ultimately makes interpreting Seahorse XF data and finding relationships amongst the data easier.
Our scientific team will be presenting posters demonstrating popular screening and imaging applications using biochemical, cell-based and live cell assays with instrumentation from BioTek.
Permanent Abstract #2548
Title: An image-based method to detect and quantify T cell mediated cytotoxicity of 2D and 3D target cell models
Session Category: Clinical Research
Session: Adoptive Cell Therapy 2
Session Date and Time: Monday Apr 16, 2018 1:00 PM - 5:00 PM
Location: McCormick Place South, Exhibit Hall A, Poster Section 24
Poster Board Number: 6
Permanent Abstract #LB-318
Title: Automated monitoring and analysis of kinetic live cell 3D spheroid-based tumor invasion within a hydrogel matrix
Session Title: Late-Breaking Research: Tumor Biology 2
Session Date and Time: Tuesday Apr 17, 2018 1:00 PM - 5:00 PM
Session Location: Poster Section 45
Poster Board Number: 10
For complete event information, visit www.aacr.org.