Translating Compounds into Therapies
Dr. Samuel Hasson is a post-doctoral fellow at the National Institutes of Health, National Center for Advancing Translational Sciences (NIH, NCATS), under the direction of Jim Inglese. The Inglese lab’s research goals include translating molecular or cellular phenotypes of human disease biology into innovative high-throughput screening (HTS) compatible drug discovery assays. Dr. Inglese’s team also seeks to develop new paradigms and technologies that advance chemical biology. Within the lab, Dr. Hasson is interested in the link between mitochondrial dysfunction and Parkinson’s disease, a chronic and progressive movement disorder, and works with cell-based and target-based assays. He created a set of high-content assays to report the modulation of mitochondrial quality control as it relates to the Parkinson’s disease pathology. These assays are being used to screen for genetic and small molecule effectors that control the cell’s ability to eliminate damaged mitochondria.
Dr. Hasson was previously acquainted with BioTek’s microplate dispensers, washers and readers, and appreciates the familiar interface of the NCATS lab’s EL406™ Combination Dispenser Washer. The lab requires dispensing and plate washing in 96-, 384-, and 1536-well microplate formats, and having this variety in one small footprint is an ideal use of lab space. Additionally, he notes that batch processing for up to 50 plates is simple and straightforward.
The lab increases efficiency by adding BioTek’s BioStack™ Microplate Stacker, allowing the EL406 to perform complex washing and dispensing protocols, including precise incubations and multiple reagent additions, in an automated manner. This compact system allows small, highly-focused chemical library screening during assay development, and Dr. Hasson notes that after installation and brief training, their team utilizes the EL406 on a daily basis.
BioTek’s Microplate Specialists provided custom modifications to the system, enabling the Inglese lab to utilize a multitude of plate types including custom formats for specialized projects. Dr. Hasson notes, "With BioTek’s EL406, I feel that we can design and execute a wide range of HTS assays because of the platform’s flexibility and modular design."
Front row, left to right: Sam Hasson, Jim Inglese, Sung-Wook Jang, Ken Cheng, and Patricia Dranchak.
Back row: Ryan Macarthur.
Thanks to Dr. Samuel Hasson at NIH, NCATS for relating his BioTek experience with us.