Aymeric Monteillier, UNIGE, Geneva, Switzerland
Discovering Nature’s Potential with Cytation 3
In the Pharmacognosy research unit of the School of Pharmaceutical Sciences at the University of Geneva in Switzerland, Dr. Muriel Cuendet’s lab searches for new cancer chemopreventive and antiparasitic agents from the broad chemical diversity found in natural sources.
As part of his PhD project in Dr. Cuendet’s lab, Aymeric Monteillier’s responsibility is to screen extracts and isolated compounds for their nuclear factor kappa B (NF-κB) inhibitory activity, and elucidate the mechanism of action of hit compounds.
BioTek’s Cytation™ 3 Cell Imaging Multi-Mode Reader (now replaced by the Cytation 1 Cell Imaging Multi-Mode Reader), with integrated Gen5™ Microplate Reader and Imager Software, was recently acquired by the lab so that Mr. Monteillier and all the researchers in Dr. Cuendet’s lab could increase throughput compared to manual methods for the many different assays performed. The assay variety includes cell viability and migration, luciferase reporter assays, gene and protein expression analyses, and fluorescence imaging. Cytation 3 is used every day, and everyone appreciates the versatility enabled by its multiple reading modes. Additionally, the instrument’s automated microscopy mode is especially well-suited to quantify nuclear translocation of NF-κB upon stimulation by tumor necrosis factor alpha (TNF-a), and its time lapse video capability is useful during cell migration studies.
The NF-κB transcription factor is involved in a number of hallmarks of cancer, including proliferation, migration, inhibition of apoptosis and angiogenesis. Therefore, it is an important target in cancer prevention and therapy. Mr. Monteillier comments, “Cytation 3, combined with its Gen5 Software, allows us to quickly and easily identify NF-κB inhibitors and characterize their mechanisms of action.” In fact, he notes that a single immunofluorescence experiment using a 96-well microplate and Cytation 3 will efficiently determine whether a compound’s effect on a target is localized in an upstream cytoplasmic pathway, such as IκB kinase (IKK) inhibition; if it impacts NF-κB p65 subunit nuclear translocation; or if it directly interacts with the DNA in the cell’s nucleus.
To learn more about UNIGE, visit their web site.
Thanks to Mr. Monteillier at UNIGE for sharing his BioTek experience.