Dr. Jorge Farías Avendaño, Director of Chemical Engineering Department, Faculty of Engineering and Sciences, Universidad de La Frontera, Temuco, Chile
Time to move forward with confidence in results
Dr. Jorge Farías studies cellular and molecular mechanisms in fish spermatozoa as part of his role as the Director of Chemical Engineering at the Universidad de La Frontera in Temuco, Chile. Spermatogenesis, which is the development of sperm, is a delicate balance between cell proliferation, differentiation and apoptosis, and disruptions can have profound implications. For example, sperm cryopreservation is a basic tool in the field of animal improvement, however, cryodamage sustained during freezing and thawing can alter or disrupt cell structure and physiology, and impact both fertilization capabilities and the resulting quality of spawn. Internal factors, such as hypoxia, may also induce adverse effects. Dr. Farías’s research aims to create actionable insights toward developing potential targets for therapeutic strategies and modifying breeding practices to ensure the quality of offspring. Additionally, his lab investigates protein engineering in yeast such as P. pastoris to improve efficiency and cost-effectiveness in medical and pharmaceutical applications. This methylotrophic yeast is amenable to genetic manipulations, with rapid growth rates in high-density fermentations.
Recently, Dr. Farías was looking for a way to complement and even replace some of the various assay techniques used throughout the lab to make better use of his team’s time. BioTek’s Synergy™ HTX Multi-Mode Reader provided the versatility that he was looking for and Dr. Farías noted that everyone appreciated Synergy HTX’s ability to measure absorbance, luminescence and fluorescence in one instrument.. Once installed, the entire team was trained on use of the instrument along with integrated Gen5™ Microplate Reader and Imager Software, This allowed the lab to run different assay types using a wide set of probes, including ELISA, enzyme activity as well as metabolite measurements. This, in turn, complemented data from other methods, including immunofluorescence microscopy, flow cytometry and PCR; and also reduced use of labor-intensive methods such as Western blotting. The Gen5 software made it easy to compare and correlate results and eliminated the need for manual manipulations and measurements. Dr. Farías is pleased with his Synergy HTX experience and its contribution towards reducing laborious and time-consuming methodologies, saying, “I feel confident because Synergy HTX’s quality means that our results are reliable.”
To learn more about Universidad de La Frontera, visit their web site.
Thanks to Dr. Jorge Farías Avendaño for sharing his BioTek experience.