Cytation™ 3 Debuts at Duke University
Dr. Rupa Ray is a Medical Instructor in Dr. Salvatore Pizzo’s pathology lab at Duke University (Durham, NC), where cancer biology and proteinase regulation are studied. Of particular interest is glucose-regulated protein 78 (GRP78), which is usually found in the endoplasmic reticulum, but is also found to translocate to tumor cell surfaces, where it acts like a growth factor-like receptor to promote tumor growth and metastasis. The Pizzo lab seeks to understand GRP78’s role in the pathophysiology of cancer, and its potential use as a cancer therapy target.
A wide variety of in vitro and in vivo methods are used to unlock GRP78’s secrets, including ELISAs, many cell-based assays, fluorescence microscopy, cell modeling, and more. The lab was in the process of evaluating a number of multi-mode microplate readers, including BioTek’s Synergy™ readers, when the local BioTek Sales Representative told them about a new combination multi-mode reader and imager, called Cytation™ 3, that BioTek was launching later in the year. The Sales Representative was able to secure a pre-release Cytation 3 unit, and Dr. Gustaaf de Ridder, a postdoc in Dr. Pizzo’s lab, thoroughly evaluated the instrument; using Cytation 3’s imaging module to follow cell morphology for long term cultures, and the multi-mode module for absorbance and luminescence assays. Along with input from the entire lab, Drs. Pizzo, Ray and de Ridder together made the decision to purchase Cytation 3. “Our experience was nothing short of perfect,” recalls Dr. Ray. “From the beginning of the process, sales was not pushy, but informative. And installation went very smoothly, especially considering that we were getting the very first Cytation 3 unit for the academic market.”
After several months of use, Dr. Ray is very satisfied with Cytation 3 and plans to expand its use to include FRET and fluorescence polarization assays in addition to its current imaging and reading functions. Dr. Ray notes that Cytation 3 “significantly reduced the time required for a number of cell culture-based assays,” and adds, "because the instrument is automated and the setting can be saved and applied to different experiments, the accuracy of our results has improved, resulting in more data." She summarizes her experience saying "With BioTek's Cytation 3, I feel empowered to do better science, because it greatly simplifies the execution of complex cell biology experiments."
To learn more about Duke University, visit their web site.
Thanks to Dr. David Sibley at Washington University for sharing his BioTek experience.