A Convenient Jump Start for Anti-tumor Immunity
Beckman Research Institute at City of Hope National Medical Center
In the Department of Immuno-Oncology in the Beckman Research Institute at City of Hope National Medical Center, Dr. Marcin Kortylewski and his group incorporate a “flank” strategy in the fight against cancer. Instead of directly focusing on tumor cells, which can evolve over time, the researchers target well-defined immune cell functions. Dr. Kortylewski explains that the immune cell protein, STAT3 (signal transducer and activator of transcription, third member of the family), plays the role of master regulator for normal immune response. STAT3 also performs a similar function when immune cells migrate into the tumor microenvironment. This creates an immunosuppressive effect that prevents a strong anti-tumor response, so blocking this protein can re-energize anti-tumor immunity. His research suggests that oligonucleotide-based therapeutics that inhibit STAT3 in a cell-selective manner may effectively restore the anti-tumor immune response.
A while back, a filter-based reader in his lab malfunctioned and eventually disappeared. As a result, the researchers were burdened with using multiple instruments on different floors and buildings to perform the wide variety of assays needed to support their functional research advanced preclinical studies.When researching replacement options, Dr. Kortylewski’s group considered a combination multi-mode instrument that didn’t require separate filters (often they get lost) and one that measured luminescence. He was intrigued to find that some readers also incorporate cellular imaging, which could benefit multiple studies in this lab including oligonucleotide labeling and modifications, cellular uptake, cell migration and cell viability.
Reliable instrument performance and technical support were quite important to his team, and they evaluated several instruments before deciding on the Cytation™ 3 Cell Imaging Multi-mode Reader. Other competitive instruments didn't provide the features or quality results they were looking for, while others lacked a solid, local support infrastructure. They did notice positive online reviews of BioTek's Cytation 3, and appreciated the modular design that would allow the instrument to easily adapt to the lab's changing needs. Once they tested the instrument, and noted the quality of reading and imaging, it was an easy decision. “Cytation 3 looked like a very solid design, guaranteed to work for each function, giving the maximum that one can achieve, at least for our needs,” Dr. Kortylewski recalls. “It seemed to fit very well.”
The entire lab was trained on Cytation 3's operation, and BioTek's technical experts returned recently to give additional refresher and in-depth training, as a “very effective way of bringing everyone up-to-date with the instrument” and to also assist with specific questions. Dr. Kortylewski summarizes the lab’s experience saying, “Now we don't have to walk around finding different instruments to do separate measurements anymore because it's all integrated in one reliable instrument, with one type of software, and within a few convenient meters of the lab.”
(Left to right): Dr. Marcin Kortylewski, Priyanka Duttagupta, Marc Lucia, Xingli Zhao, Dayson Moreira
To learn more about the Beckman Research Institute at City of Hope National Medical Center, visit their website.
Thanks to Dr. Marcin Kortylewski at Beckman Research Institute for relating his BioTek experience with us.