Adding Automated Value to Specialty Zebrafish Assays
The city of San Sebastián-Donostia is nestled along the picturesque northern coast of Spain, and is also home to the Contract Research Organization, Biobide. For more than twelve years, Biobide has applied their expertise in the zebrafish animal model to accelerate research and development processes for pharmaceutical, biotechnology, chemical, cosmetic and nutraceutical companies. Their services include developing teratotoxicity, neurotoxicity, cardiotoxicity, hepatotoxicity and other toxicity assays, central nervous system, oncology, immune system related efficacy assays, as well as disease model generation; all as part of preclinical research.
As each assay is customized to client requirements, Biobide’s R&D department, including Maria Virginia Caballero Ph.D., Study Director and Iñaki Iturria, R&D Scientist, needed a robust automation workhorse that could handle diverse performance requirements without slowing development time. In the course of evaluating instruments, their local distributor introduced them to BioTek’s Cytation™ 3 Cell Imaging Multi-Mode Reader. They purchased the Cytation 3 in September 2016, citing Cytation 3’s ability to combine image acquisition with fluorescence and absorbance quantification, while also complementing their existing imaging hardware. Dr. Caballero notes, “Cytation 3 was the best choice for us as it handles a broad range of image capture requirements along with High Content Screening capabilities.”
After initial training, users found Cytation 3 and its integrated Gen5™ Microplate Reader and Imager Software to be intuitive and user-friendly, especially when used on assays that require verification of imaging and reading at the same time. The increased throughput and reduced need for manual analysis is a boost for productivity while yielding consistent results. Thanks to Cytation 3, Biobide can feel technically supported and confident in results as they continue to develop tailor-made assays and disease models to benefit the therapeutic initiatives of their clients.
Biobide’s zebrafish facility
Live transgenic zebrafish larvae with red fluorescent liver and green fluorescent pancreas. Image courtesy of Biobide.
To learn more about Biobide visit their web site.
Thanks to Maria Virginia Caballero at Biobide for sharing her BioTek experience.