BioTek: Microplate Instrumentation & Software Solutions
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FAQ

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Absorbance Readers

How can we verify that the measurements above the OD accuracy, linearity and repeatability specification are acceptable for our lab?

Why is the OD accuracy, linearity and precision specified for a smaller range than the defined dynamic range?

What are the filters/mirror and reading parameters required to read AlphaScreen and AlphaLISA assays on my Synergy?

What are the filters/mirror and reading parameters required to read HTRF® assays on my Synergy?

Which printers are compatible with my ELx800 or ELx808 reader?

What is the expected lifetime of the Xenon Flash lamp?

What volume should I use for DNA quantitation when reading at 260 nm?

Do I have to use a blank on the plate when quantitating DNA at 260 nm?

What advantages does the Bio-Cell™ offer?

Can I use pathlength correction wavelengths other than 977 nm for aqueous solutions?

My DNA quantitation results are quite different than that provided by my spectrophotometer

What are the commonly used ELISA conjugate/substrate combinations and what wavelengths do they require?

How can I run cell growth assays on my absorbance microplate reader?

Fluorescence Readers

What are the benefits to Top Reading versus Bottom Reading on my fluorescence microplate reader?

Luminescence Readers

How do I configure my Synergy HT / FLx800 for luminescence?

Multi-Mode Readers

How do I choose between monochromators or filters?

How long should my lamp last and how do I change it?

Fluorescence filters: when you see 485/20 nm for a filter does it mean that the band pass is 20 nm in total or +/- 20 nm?

What microplates can I use when reading absorbance of samples in the UV range?

Do the requirements for successful DNA quantitation including pathlength correction, appropriate plates, minimum volumes, and blank wells apply to RNA and protein quantitation at 260 and 280 nm respectively?

How do I optimize the instrument for reading assays with live cells (cellular assays) on the bottom of the wells?

How do I adjust the sensitivity for optimum results in fluorescence or luminescence?

Can I read PCR plates on my FLx800 or Synergy microplate reader?

Will my ELx808 or PowerWave 340 work with plates that are not 96-wells?

General Question

What is the range on my absorbance or fluorescence filter?

When will my BioTek product be compliant with RoHS2 Directive 2011/65/EU?

Is refurbished or used microplate instrumentation available?

Where do I look for information regarding instrument maintenance?

Discontinued BioTek Products

We have a Synergy HT and wish to know if turning the unit off at night will affect lamp life or not. We'd like to decrease overall utility bills by all means possible.

What should I do if I have a problem or question about my BioTek equipment?

How do I troubleshoot communication problems between my reader and computer?

How does the Universal Test plate analysis determine the pass/fail criteria for accuracy and repeatability?

How often should I perform performance qualification tests?

What is the detection limit for DNA quantitation?

I need an electronic copy of an Operator's manual. Where do I get one?

What is the MSDS information for BioTek QC test solutions?

How can I connect a BioTek instrument to my laptop that only has USB ports?

What is the best way to store ELx800 and ELx808 interference filters for extended periods of time?

How are BioTek readers able to read larger diameter wells?

What’s the best way to handle assays with temperature control?

What are the default parameters for BioTek’s microplate readers in Fluorescence mode?

What solutions do you offer to automate hit picking applications?

Does BioTek offer refillable pipette tip formats for environmental and cost-savings purposes?

What microplate should I use for a particular assay?

How can I compensate for condensation build-up on the underside of a microplate lid with an assay requiring incubation?

How do I get my "universal absorbance test plate" recertified?

Microplate Dispensers

I need to dispense 750 µL to a 96-well format deep well microplate. Which of BioTek's microplate dispensers, the MicroFill or the MicroFlo Select, would be the fastest? If it is the MicroFlo Select, would the 5 µL or the 10 µL cassette be best?

I am currently using a BioTek ELx405 plate washer and am having some clogging issues with the aspiration manifold. Is there a wire tool available to manually clear the aspiration tips? What other methods do you recommend to clean the manifold?

Microplate Washers

Can I use the vacuum filtration feature of my ELx50 strip washer for partial plates?

Which magnet should I choose for my BioTek washer?

Can I transfer and share protocols between my BioTek washers and dispensers?

The buffer I am using does not contain surfactant, how can I optimize aspiration in a 384-well plate?

What is AutoPrime™ and when should I use it?

Can I use acid or alkali solutions with BioTek's ELx50 Washer?

If I want to use a house vacuum, what is the vacuum requirement for the ELx405?

I may be interested in using my instrument to dispense a corrosive chemical. Are there certain chemicals that should not be used with your instruments?

When I’m using solutions that contain proteins, how often should I perform a maintenance routine on my microplate washer?

How do you recommend maintaining my ELx405 Microplate Washer under sterile conditions?

Why does my liquid handler come on by itself about 10 minutes (or more) after a run is complete?

Pipetting Systems

Is the Precision compatible with any vacuum manifolds?

How often should I replace the pipette o-rings on my Precision Microplate Pipetting System?

Robotics

Can BioStack 4 be controlled via the touch screen interface of 405TS and MultiFlo FX to work with both lidded and non-lidded plates?

Can I use the BioStack 4 with Gen5 v2.04?

Can I use the standard BioStack stacks (p/n 7310008, 7310023 and 731003) with BioStack 4?

Can BioStack 4 be controlled via the keypad (non-touch screen) liquid handling instruments (EL406, 405LS, MultiFlo)?

Software

What does it mean when I get the value "OVRFLW" on my Gen 5 results and how can it be corrected?

How do I transfer Gen5 to a new computer?

How can I update my Gen5 software?

What is a “discontinuous kinetic” procedure in Gen5?

Can I copy and paste data into Gen5?

My lab is 21 CFR Part 11 compliant, what products do you have for me?

Is it possible to pre-heat the incubator and warm up the tungsten bulb in my Synergy reader?

What file types can I import into Gen5 software for analysis?

Where can I find Sample Files for Gen5?

How do I register and get a password for my software?

What is the Gen5 Diagnostics Utility, and is it necessary in order to use the Absorbance Test Plate?

How do I import data into Gen5?

How do I export data from Gen5 into Excel using PowerExports?

I’m using Gen5 Software; am I limited to operating one microplate reader at a time?

Can I install Gen5 on a computer not connected to my plate reader?

What do I do if I mistakenly register KC4 using the Network license instead of the Full license?

Do I need to uninstall the previous version of KC4 before I install my new version?

With KC4 Signature, can I report or export using PowerReports and still maintain compliance to 21 CFR Part 11?

Incorrect or missing MSJET DLL file error message

Can the ELx800/ELx808/µQuant, etc (instruments with keypads) send data to my LIMS system?

I have Microsoft Windows Vista on my computer. How can I get Vista compatible USB drivers for my system?