Applications - Presentations
The Synergy Family of Readers; A Lower Cost High Performance Alternative for Running AlphaScreen Assays
Authors: Paul Held, BioTek Instruments; Daniel D'Orazio, Novartis Pharma AG
AlphaScreen® assays are a homogeneous assay technology that is effective for screening a broad range of targets. This assay technology has been adapted to measure cAMP; Insulin; kinase activity; TNF-α; and IP3 to name a few analytes. AlphaScreen® assays have historically been run using expensive detection systems that utilize a dedicated laser for excitatory light. Monochromatic lasers provide intense continuous light output as well as wavelength specificity, both of which are necessary for the AlphaScreen® assay. Xenon flash lamps, which are often used in multidetection microplate readers, provide light output over a broad wavelength range, but also cease illuminating very rapidly as required for time resolved measurements. Unfortunately, a xenon flash lamp does not provide the continuous illumination necessary for AlphaScreen® assays.The interval between flashes allows the excitatory reaction to diminish. Unlike most microplate readers, the BioTek Synergy 2 and Synergy 4 multi-mode readers incorporate both a constant light source as well as a xenon-flash lamp. Their unique multi-optic design provides continuous excitation, wavelength specificity, and high sensitivity necessary for these assays to be run effectively. Here we describe the data from several typical AlphaScreen® assays, including cAMP; insulin; TNF-α; as well as pJNK and p38MAPK phosphorylation, demonstrating the ability of the Synergy readers to perform this popular assay technology. Examples of typical performance of these assays will be provided along with an overview of both the assay technology and reader design.
Synergy 2 Multi-Mode Microplate Reader.
AlphaScreen® is a registered trademark of PerkinElmer Inc.