Applications - Presentations
Synergy™ 2 Multi-Mode Microplate Reader: A Novel Approach to Detection for HTS and Drug Discovery
Authors: Paul Held and Xavier Amouretti, BioTek Instruments, Inc., Winooski, VT 05404
This poster was presented at Drug Discovery & Technology 2006 and the Society for Biomolecular Screening 2006.
The Synergy™ 2 is a new type of reader that provides research laboratories performances usually found on high-end HTS instrumentation, while delivering flexibility and efficient cost-control to screening laboratories. The Synergy 2 utilizes multiple optics to provide performance regardless of the detection technology. Absorbance measurements use a xenon lamp with a monochromator for wavelength selection from 200 nm to 999 nm. Fluorescence measurements are made using either a tungsten-halogen or a xenon-flash lamp with filters and dichroic mirrors and a PMT for detection. Fluorescence polarization is accomplished with polarizing filters in conjunction with dichroic mirrors. For time-resolved fluorescence measurements, the Synergy 2 integrates a high-energy xenon-flash lamp with excitation and emission filters and PMT detector. Luminescence measurements are accomplished by using a liquid-filled light guide. The Synergy 2 is capable of reading any plate format up to 1536 wells, is robotic compatible and provides temperature control and shaking. Reagent injectors are also available.
Figure 1. Synergy™ 2 Multi-Detection Microplate Reader.