Applications - Presentations
Automated ELISA Liquid Handling with EL406 Combination Microplate Washer Dispenser
29-Apr-09BioStack , EL406
Authors: Paul Held, Jason Greene, Gary Barush and Peter Banks, BioTek Instruments
With a typical ELISA protocol, several repeat cycles of microplate washing, reagent addition and incubation are< executed to remove unbound material before data collection. This often manual process requires a technician to manage timings and be available to move the plate between washer and multiple dispensers. The EL406 Microplate Washer Dispenser increases a laboratory’s efficiency and productivity by eliminating these manual plate movements. This automation friendly, multifunctional instrument uniquely combines the industry standard ELx405 Select CW Washer with a peristaltic reagent pumping system and two highly accurate syringe drive dispensers. The wash module incorporates BioTek’s patented Dual-Action™ manifold design for both 96- and 384-well microplates along with optimized flow rates for cell-based applications. The peristaltic pump utilizes a unique design to provide accurate and precise volumes ranging from 1 μL to 3 mL. With two optional syringe pump dispensers, a total of three reagents can be dispensed without operator intervention. Additionally, up to four reagent buffers can be used automatically. Together with a BioStack Microplate Stacker, as a batch processor the EL406 will manage all liquid handling steps of an ELISA inclusive of incubations. Users simply press a button and walk away eliminating the need for multiple instruments and saving both bench space and cost. This poster will detail specifics of such a system.
EL406 Combination Washer Dispenser