Applications - Presentations

Analytical Performance of a Multi-Volume Microplate Accessory for Microplate Spectrophotometer Platforms

09-Mar-10

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Related Products: Epoch, Take3

 

Authors: Peter Brescia, Mike Sevigny and Peter Banks, BioTek Instruments, Inc., Winooski, VT, USA

 

Abstract

 

Accurate determination of molecular concentrations is a prerequisite to the use of purified biomolecules for a multitude of downstream applications. Quantifications is routinely accomplished by spectrophotometric analysis. BioTek recently introduced an accessory for its microplate spectrophotometers that can accommodate a range of liquid volumes and samples for spectrophotometric analysis. The new Take3™ Multi-Volume Plate incorporates the ability to measure up to sixteen low-volume (2µL) samples for direct quantification, two vertical 1 cm pathlength cells, and one standard 1 cm cuvette, in a standard microplate sized format. Its use allows a researcher to quantify samples without dilution while also allowing more dilute samples to be read in a standard 1 cm pathlength format.

Spectrophotometric measurements are derived from the low volume microspots, minimum 2µL, a 1 cm pathlength vertical quartz cuvette a 1 cm pathlength cuvette on board the accessory. The analytical performance of the accessory will be discussed to include limit of detection, dynamic range and accuracy for proteins and nucleic acids.

 

 



Epoch Multi-Volume Spectrophotometer System



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