BioTek: Microplate Instrumentation & Software Solutions
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Technical Resources

Technical resources are available to illustrate product specific capabilities and common applications for BioTek microplate instrumentation. These documents assist with both the operation of BioTek instrumentation to achieve peak performance, and their implementation in common experimental design.
 
 


ADME/Tox

17-Feb-10 Automated Drug-Induced Chromatin Condensation Detection
Detection of End-Stage Apoptosis Using the Nuclear-ID™ Kit from Enzo Life Sciences
10-Dec-09 Automation of a Multiplexed Cell-based Assay to Measure Simultaneously Induction of Gene Expression and Activity of Cytochrome P450 Isoform 3A4 by Small Molecule Compounds
31-Mar-09 Automated ApoTox-Glo Assay to Assess Cell Viability, Cytotoxicity and Apoptosis
20-Mar-09 An Absorbance-based Cytotoxicity Assay using High Absorptivity, Water-soluble Tetrazolium Salts - Cell Quantitation Using WST-8 and the Synergy™ Mx
21-Jul-08 Invitrogen's Predictor™ hERG Fluorescence Polarization Assay Using BioTek's Synergy™ 4 Hybrid Microplate Reader - Non-Radioactive High Throughput Screening Assay for hERG Binding
28-Jul-04 Caspase Activity Using Synergy HT Multi-Mode Microplate Reader: Measuring Apoptosis Enzymes

Automation and Liquid Handling

18-Feb-10 Automated Cell Dispensing into 1536-Well Microplates for HTS - Using the MicroFlo™ Select to Seed Tissue Culture Cells
28-Jan-10 Versatile, Cost-Effective Automation of Avian Influenza and Mycoplasma Gallispeticum-Synovaie ELISAs
06-Nov-09 Automated Workflows for Luminex xMAP® Assays
16-Jan-09 Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation
15-Jan-09 Automated ELISA Liquid Handling with the EL406™ Combination Washer Dispenser - Semi-Automation of ELISA Process
28-Apr-08 Aliquoting Agar Containing Solutions with the MicroFlo™ Select Dispenser
04-Mar-08 Automating the IDEXX Avian Influenza Virus Antibody Test Kit with Microplate Instrumentation and BioTek Twister II
16-Apr-07 Luminex xMAP Assays: Using BioTek’s ELx50™ Filter Microplate Washer to Perform the Wash Processes Required
19-Sep-06 Automating the Wash Steps of ELISPOT Assays using BioTek’s ELx405™ Microplate Washer
24-Sep-01 Automating Routine Microplate Pipetting and Dispensing

Biomarker Quantification

22-Sep-09 Automated Magnetic Bead Washing of Metabolic Hormone Assays - Using the ELx405™ Magnetic Bead Washer to Wash MILLIPLEX™ MAP Assays
18-Sep-09 Automated Washing of Magnetic Bead QuantiGene® Plex 2.0 Assays - Using the ELx405™ Magnetic Bead Washer to Wash Multiplexed mRNA Quantitation Assays
01-Jul-09 Determination of Insulin Levels in Human Serum - Using the ELx405™ Microplate Washer and the Synergy™ Mx Monochromator- Based Multi-Mode Microplate Reader to Perform an Insulin ELISA
21-May-08 Quantitation of Human TNF-a with a QuantiGlo Assay from R&D Systems Using BioTek’s Synergy™ 2 Multi-Mode Microplate Reader - Luminescent ELISA Measurements
10-Dec-07 AlphaLISA: Human Insulin Quantification Using Synergy 4 Hybrid Multi-Mode Microplate Reader
26-Mar-07 cAMP and Tumor Necrosis Factor Quantification Using Synergy 2 Multi-Mode Microplate Reader and HTRF® Assays for High-Throughput Screening
28-Feb-05 Cholesterol Quantification Using a Fluorometric Assay and Synergy HT Multi-Mode Microplate Reader

Cell Biology and Enzymology

10-Dec-09 Quantifying Cytotoxicity of Thiostrepton on Mesothelioma Cells using MTT Assay and the Epoch™ Microplate Spectrophotometer
05-Nov-08 Using BioTek's Synergy HT Reader to Measure Reactive Oxygen Species (ROS) Generation in Stimulated Cells
26-Nov-07 Determinating NADH Concentrations with Synergy™ 2 Multi-Mode Microplate Reader using Fluorescence or Absorbance
26-Feb-07 Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software
22-Jun-04 Luminescent Determination of ATP Concentrations Using the Clarity™ Luminescence Microplate Reader
09-Jun-04 Luciferase Measurements Using the Clarity™ Luminescence Microplate Reader
21-Apr-03 Horseradish Peroxidase (HRP) Determination Using Amplex Red and Synergy HT Multi-Mode Microplate Reader
20-Aug-02 Hydrogen Peroxide Quantification Using Synergy HT Multi-Mode Microplate Reader
24-Sep-01 ATPase Activity Determination Using BioTek's ELx808 Microplate Reader
20-Feb-01 ß-Galactosidase Activity Determination using a BioTek Microplate Fluorescence Reader
07-Feb-01 BioTek PowerWave 200 Microplate Spectrophotometer: Nitric Oxide Synthesis
06-Feb-01 Phosphate and ATPase Determination Using the µQuant Scanning Microplate Spectrophotometer

Drug Discovery

24-Feb-10 Utility of Synergy™ H4 Hybrid Multi-Mode Microplate Reader using both Monochromator- and Filter-Based Detection - Capabilities demonstrated with Fluorescence Polarization, Time-Resolved Fluorescence Resonance Energy Transfer, and Fluorescence Intensity Assays for the Measurement of ADP Accumulation
01-Feb-10 Automated Processing of a Homogeneous Phospho-ERK1/2 Assay for Use as a Generic GPCR Sensor - Homogeneous LanthaScreen® Cellular Assay
22-Dec-09 BacMam-Enabled Cellular Assays Measuring Histone Posttranslational Modifications
10-Jul-09 Optimization and Validation of Millipore’s HTRF® PI3 Kinase Assay for Inhibitors to Class IA Phosphionositide 3-Kinase Isoform Activity
01-Jul-09 Cell-Based Assay Preparation in 1536-well Microplates using BioTek’s EL406™ Microplate Washer Dispenser
17-Jun-09 HTRF® cAMP/IP-One HTplex™ Cell-Based Assay performed on BioTek’s Synergy™ 2 Multi-Mode Microplate Reader
09-Apr-09 Optimizing Performance of the Membrane-free, Oris™ Cell Migration Assay for High Throughput Screening using the BioTek Synergy™ HT Multi-Mode Microplate Reader
10-Oct-08 LanthaScreen™ TR-FRET Assay from Invitrogen on Synergy 4
26-Sep-08 Determination of Relative Potency Using Parallel Line Analysis with Gen5 Data Analysis Software
26-Sep-08 Gyrasol Kinase Assays
17-Sep-08 Optimizing Performance of the Transcreener® ADP Assay for the BioTek Synergy 2 and Synergy 4 Multi-Mode Microplate Readers
21-Aug-08 Oris - A Novel High Throughput-Compatible Cell Migration Screening Assay
20-May-08 Invitrogen’s Adapta™ Universal Kinase Assay using BioTek’s Synergy™ 4 Hybrid Microplate Reader
20-Dec-07 GeneBLAzer® FRET Cell-based Assay from Invitrogen™ on Synergy™ 4 Hybrid Multi-Mode Microplate Reader
19-Dec-07 Omnia Kinase Assay from Invitrogen on Synergy 4
10-Dec-07 AlphaScreen™: cAMP Quantification Using Synergy 2 Multi-Mode Microplate Reader
15-Nov-07 Z’-LYTE® Kinase Assay from Invitrogen™ on Synergy™ 4 Multi-Mode Microplate Reader
01-Mar-05 FRET-Based Ion Channel, GPCR, and Kinase Assay Applications on the Synergy™ HT Multi-Detection Microplate Reader from BioTek
23-Feb-05 Optimizing Dual-Glo™ Luciferase Assays with Synergy™ HT Multi-Mode Microplate Reader
15-Feb-05 Using Synergy™ HT Multi-Mode Microplate Reader to Run the Dual-Luciferase® Reporter Assay System
09-Jun-04 Using the Clarity™ Luminescence Microplate Reader to Perform Dual-Luciferase® Assays
29-Jan-04 Ion Channel Assay Development on the Synergy™ HT Multi Detection Microplate Reader from BioTek
06-Jun-03 Detecting Calcium Concentration Changes Using the FLx800 Fluorescence Microplate Reader

Food Contaminant & Microbial Quantification

05-Mar-09 Identification of Biofuel Producing Bacteria Through Temperature Resistance - Demonstration of the High Temperature Capabilities of BioTek’s Microplate Readers
16-Jan-09 Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation
15-Jan-09 Automated ELISA Liquid Handling with the EL406™ Combination Washer Dispenser - Semi-Automation of ELISA Process
16-May-08 Fluorescence Polarization Detection of Brucella abortus and Mycobacterium bovis specific antibodies Using Kits from Diachemix
15-Jan-08 Monitoring the Growth of E. coli With Light Scattering Using the Synergy™ 4 Multi-Mode Microplate Reader with Hybrid Technology™
08-Dec-06 Mycoplasma Detection Using Synergy™ 2 Multi-Mode Microplate Reader and the Lonza MycoAlert Assay
29-Aug-06 Histamine Analysis in Wine Samples Using the Microplate Format
17-Aug-05 Performing Oxygen Radical Absorbance Capacity (ORAC) Assays with Synergy™ HT Multi-Mode Microplate Reader - ORAC Antioxidant Tests

Immunoassays

01-Jul-09 Determination of Insulin Levels in Human Serum - Using the ELx405™ Microplate Washer and the Synergy™ Mx Monochromator- Based Multi-Mode Microplate Reader to Perform an Insulin ELISA
16-Jan-09 Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation
15-Jan-09 Automated ELISA Liquid Handling with the EL406™ Combination Washer Dispenser - Semi-Automation of ELISA Process

Nucleic Acid Quantification

09-Dec-09 Analytical Performance of Nucleic Acid Micro-Volume Quantification Using the Epoch™ Spectrophotometer System
09-Nov-09 Compatibility of Synergy™ Multi-Mode Microplate Readers for Micro-Volume Nucleic Acid Quantification
03-Nov-09 Multi-Volume Analysis of Nucleic Acids Using the Epoch™ Spectrophotometer System
17-Aug-09 Low Volume Nucleic Acid Quantification using a Multi-Volume Spectrophotometer System
09-Jan-09 BioTek Synergy™ 4 Hybrid Microplate Reader
04-Dec-06 Fluorometric dsDNA Quantification Using PicoGreen®
05-Sep-03 RNA Quantification with RiboGreen Stain Using the FL600 Fluorescence Microplate Reader
21-Feb-01 Nucleic Acid & Protein Quantitation in the Microplate Format
21-Feb-01 ssDNA Quantification Using OliGreen Fluorescent Stain
21-Feb-01 DNA Quantification using Hoechst 33258
20-Feb-01 Fluorometric Hydrolyzed DNA Quantification
09-Feb-01 Increasing the DNA Quantification Range when using Hoechst Dye 33258
06-Feb-01 Nucleic Acid Purity Assessment Using A260/A280 Ratios
06-Feb-01 BioTek PowerWave HT Microplate Spectrophotometer: Quantitation of Nucleic Acids

Protein Quantification

26-Feb-10 In-situ Micro-Volume Bicinchoninic Acid Protein Assay
21-Jan-10 Analytical Performance of Epoch™ Multi-Volume Spectrophotometer System for Protein Quantification
18-Apr-03 Peptide and Amino Acid Quantification Using UV Fluorescence in Synergy HT Multi-Mode Microplate Reader
21-Feb-01 Nucleic Acid & Protein Quantitation in the Microplate Format
21-Feb-01 Fluorometric Protein Quantification using NanoOrange
20-Feb-01 Total Protein Quantification using OPA
20-Feb-01 Green Fluorescent Protein Quantification in Microplates
20-Feb-01 Fluorescence-based Total Protein Determination using the Compound CBQCA
20-Feb-01 Fluorimetric Protein Quantification Using the Reactive Compound Fluorescamine
20-Feb-01 Total Protein Determination By the Lowry Method Using the ELx808