Microplate instruments are used in a vast number of applications across multiple scientific disciplines. Through our Webinar presentations, BioTek seeks to share information and experiences with attendees about timely and important microplate-based applications. Sign up today and check back for future topics that may be of interest to you.
"A New Look at Cell-based Assays in a Multi-Mode Microplate Reader using Fluorescence Microscopy "
Caleb Foster and Paul Held, BioTek Instruments Inc.
Multi-mode microplate readers are a useful tool for cell-based assays. These instruments use PMT-based optics to provide a cell population-averaged assay signal relative to assay controls that can quantify biomolecule expression, activation, inhibition and post-translational modulation. Fluorescence microscopy allows the analysis of sub-populations of cells or even analysis of a single cell, depending on the magnification used.
In this webinar we will demonstrate this dual capability using the Cytation™3 Cell imaging Multi-Mode Reader, which incorporates automated digital widefield fluorescence microscopy to perform sub-population analysis of cells in microplates. In addition, the PMT-based optics are used in conjunction with fluorescence microscopy to enable both cell population and sub-population analysis. Examples presented will include quantification of transfection efficiency, target-based inhibition studies with accompanying cell viability determination and phenotypic assays of oxidative stress and hypoxia.
Registration is required to attend this event. Please register now.
Thursday, June, 27 2013
12:00 - 1:00 PM EST
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