Synergy Mx Monochromator-Based Multi-Mode Microplate Reader

Detection > Monochromator-based Multi-Mode Microplate Readers

Overview:

Ultra Fine-Tuned™ technology is what sets the Synergy Mx Multi-Mode Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 µm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek’s exclusive focus on microplate instrumentation and software make the Synergy Mx the most precise, sensitive and flexible multi-mode microplate reader available today for life-science research applications.

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Features:

Fully monochromator-based with quadruple grating architecture for Ultra Fine-Tuned performance.
Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
High performance fluorescence (top and bottom), absorbance and dedicated luminescence detection systems accommodate all life-science research microplate applications.
Variable bandpass selection adds flexibility in fluorescence mode.
Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
Cuvette port to measure standard 1-cm path cuvettes if only a few samples need to be run, or to compare data with a standard cuvette spectrophotometer.
Modular architecture allows purchase of required modes with the ability to upgrade later.
Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays or luminescent flash ATP assays.

Models:

Synergy Mx is a modular instrument. Read modes and options are available as individual modules at the time of purchase, allowing you to make the best use of your budget. In addition, existing units can be upgraded later when your research requirements expand.

Synergy™ Mx Model Configurations Contact BioTek or use Quote Configurator for part #
Module	Description
SM	Synergy Mx base. Includes incubation to 65°C and Gen5™ Software.
A	Microplate-based UV-Visible absorbance. Includes xenon flash lamp, dual grating monochromator system, fibers and silicon photodiode detector.
T	Top monochromator-based fluorescence intensity. Includes Xenon Flash lamp, dual grating monochromator system (excitation and emission), top fiber and dedicated red-shifted PMT detector.
B	Bottom monochromator-based fluorescence intensity. Requires top optical system installed. Includes high quality quartz fiber optics and focusing system.
C	Cuvette-based absorbance measurements. Requires “A” module installed. Includes cuvette port and holder, fiber optics and dedicated silicon photodiode detector.
L	Luminescence system. Includes dedicated luminescence optics with low-noise PMT detector.
D	Dual Reagent Dispenser

Specifications:

General
Detection mode	Fluorescence, UV-Visible Absorbance, Luminescence, Time-Resolved Fluorescence (secondary mode)
Read method	End point, kinetic, spectral scanning, well-area scanning
Microplate types	1- to 384-well plates and PCR plates Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control	4°C above ambient to 65°C +0.5°C at 37°C
Shaking	Yes
Software	Gen5™ included Control through USB or serial port
Automation	Yes
Absorbance
Light source	High Energy Xenon Flash
Wavelength selection	Double Monochromator
Wavelength range	230 - 999 nm, 1 nm increment
Bandpass	2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	Yes
Monochromator wavelength accuracy	+2 nm
Monochromator wavelength repeatability	+0.2 nm
OD accuracy	<1% at 2.0 OD typical
OD linearity	<1% from 0 to 3.0 OD typical
OD repeatability	<0.5% at 2.0 OD typical
Reading speed	96 wells: 11 seconds 384 wells: 22 seconds
Fluorescence Intensity
Sensitivity	Fluorescein 2 pM typical (0.2 fmol/well 384-well plate) (Top) Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) (Bottom)
Light source	High Energy Xenon Flash
Wavelength selection	Double grating monochromators (Top and Bottom)
Wavelength range	250-850 nm
Dynamic range	5 decades
Bandpass	Variable: 9, 13.5, 17, 20 nm
Detection system	Dedicated red shifted PMT
Reading speed	96: 11 seconds 384: 22 seconds
Luminescence
Sensitivity	10 amol ATP typical (flash)
Wavelength range	300 - 700 nm
Dynamic range	>6 decades
Detection system	Low noise PMT
Reagent Dispensers
Number	2 syringe pumps
Dispense volume	5 - 1000 µL in 1 µL increment
Dead volume	1.1 mL, 100 µL with back flush
Physical Characteristics
Power	250 Watts max
Dimensions	17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight	70 lbs (31.5 kg)
Regulatory
Regulatory	CE and TUV marked. In Vitro Diagnostic use models are available.