Synergy Mx Monochromator-Based Multi-Mode Microplate Reader

Detection > Monochromator-based Multi-Mode Microplate Readers

Synergy Mx Monochromator-Based Multi-Mode Microplate Reader


Ultra Fine-Tuned™ technology is what sets the Synergy Mx Multi-Mode Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 µm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek’s exclusive focus on microplate instrumentation and software make the Synergy Mx the most precise, sensitive and flexible multi-mode microplate reader available today for life-science research applications.

US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.

 Take3 Trio Compatible



Synergy Mx is a modular instrument. Read modes and options are available as individual modules at the time of purchase, allowing you to make the best use of your budget. In addition, existing units can be upgraded later when your research requirements expand.

 Synergy™ Mx Model Configurations
 Contact BioTek or use Quote Configurator for part #
 Module  Description
 SM Synergy Mx base. Includes incubation to 65°C and Gen5™ Software.
 A Microplate-based UV-Visible absorbance. Includes xenon flash lamp, dual grating monochromator system, fibers and silicon photodiode detector.
 T Top monochromator-based fluorescence intensity. Includes Xenon Flash lamp, dual grating monochromator system (excitation and emission), top fiber and dedicated red-shifted PMT detector.
 B Bottom monochromator-based fluorescence intensity. Requires top optical system installed. Includes high quality quartz fiber optics and focusing system.
 C Cuvette-based absorbance measurements. Requires “A” module installed. Includes cuvette port and holder, fiber optics and dedicated silicon photodiode detector.
 L Luminescence system. Includes dedicated luminescence optics with low-noise PMT detector.
 D Dual Reagent Dispenser


Detection mode Fluorescence, UV-Visible Absorbance, Luminescence, Time-Resolved Fluorescence (secondary mode)
Read methods End point, kinetic, spectral scanning, well-area scanning
Microplate types 1- to 384-well plates and PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4°C above ambient to 65°C +0.5°C at 37°C
Shaking Yes
Software Gen5™ included
Control through USB or serial port
Automation Yes
Light source High Energy Xenon Flash
Wavelength selection Double Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Reading speed 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Sensitivity Fluorescein 2 pM typical (0.2 fmol/well 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) (Bottom)
Light source High Energy Xenon Flash
Wavelength selection Double grating monochromators (Top and Bottom)
Wavelength range 250-850 nm
Dynamic range 5 decades
Bandwidth Variable: 9, 13.5, 17, 20 nm
Detection system Dedicated red shifted PMT
Reading speed 96: 11 seconds
384: 22 seconds
Sensitivity 10 amol ATP typical (flash)
100 amol ATP (glow) typical
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Detection system Low noise PMT
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 70 lbs (31.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.