Synergy Neo Multi-Mode Reader

Detection > Hybrid Technology Multi-Mode Microplate Readers

Synergy Neo Multi-Mode Reader


Select ScienceSynergy™ Neo is a hybrid multi-mode microplate reader designed specifically for today's screening and core laboratories. Synergy Neo has all the features expected in an HTS screening instrument, including multiple parallel detectors for ultra-fast measurements and a dedicated filter-based system for live cell assays. With a load/unload plate transfer time of about 6 seconds per plate, Synergy Neo's optional integrated plate stacker is the fastest stacker on the market, allowing walk-away automation of short- or long-term assays. Synergy Neo also meets today's diverse assay requirements by incorporating features such as a powerful 100 mW laser-based excitation source for Alpha assays, a unique patented Hybrid optical design, advanced temperature control to 65°C, orbital and linear shaking. Ease of use and user confidence are key to the design of Synergy Neo's unique filter cubes that are barcode labeled for positive filter ID - limiting the possibility of errors and streamlining the workflow, while an available image-based barcode reader can scan both 1D and 2D barcode-labeled plates. The outstanding functionality of Synergy Neo is controlled by the powerful, yet easy to use, Gen5 2.0 Data Analysis Software.

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.

HTRF® and the HTRF logo are registered trademarks of Cisbio.


Fiber-free top optics light path with dual PMT detectors
Alpha technologies are registered trademarks of PerkinElmer, Inc.


Part #
Top monochromator optics
Top filter optics
Top dual PMT
Bottom monochromator optics
Bottom filter optics
Alpha laser
Fluorescence intensity,
fluorescence polarization,
time-resolved fluorescence,
UV-Vis absorbance, luminescence
BioStackNeo, BioStack4 and BioStack3 compatible


Wavelength selection Hybrid Technology™ (patented)
Quadruple Monochromators and Filter Cubes
Detection modes Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, Alpha, Luminescence, UV-Visible Absorbance
Read methods End point, kinetic, spectral scanning, well area scanning
Microplate types 1- to 1536-well plates
Other labware Take3™ Micro-Volume Plates
Temperature control 4-Zone™ incubation to 65 °C; variation +0.2 °C at 37 °C with Condensation Control™
Shaking Linear, orbital, double orbital
Software Gen5™ Data Analysis Software
Automation BioStack™ and 3rd party automation compatible
Read height Auto Z, 0.1 mm steps, top/bottom (filters), top (mono)
Kinetic speed 96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
With Stacker, minimum processing time per plate 96 well: 20 seconds
384 well: 25 seconds
1536 well: 39 seconds
Barcode reader Multi-directional, 1D and 2D camera-based
Light source Xenon flash
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandwidth 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Fluorescence Intensity
Light source Xenon flash
Sensitivity Monochromator:
Fluorescein 2 pM - top
Fluorescein 2.5 pM - bottom

Filter cubes:
Fluorescein 0.2 pM (384-well low volume plate) - top
Fluorescein 1 pM (1536-well plate) - top
Fluorescein 1 pM (384-well low volume plate) - bottom
Wavelength selection Double grating monochromators (top/bottom)
Filter cubes (top/bottom)
Wavelength range Monochromators: 250 - 850 nm
Filter Cubes: 230 - 850 nm
Bandwidth Monochromators: 16 nm excitation / emission
Filters: filter-dependant, from 5 nm to >100 nm
Detection system Two matched PMTs top filter system
Low Noise PMT bottom filter system
Red shifted PMT top/bottom monochromator system
Sensitivity 5 amol ATP typical (384-well low volume plate)
Wavelength range 300 - 700 nm
Dynamic range > 6 decades
Fluorescence Polarization
Light source Xenon flash
Sensitivity 1 mP standard deviation at 1 nM fluorescein
(384-well low volume plate)
1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
Wavelength selection Filter cubes (top / bottom)
Wavelength range 280 - 850 nm
Time-Resolved Fluorescence
Light source Xenon flash
Sensitivity Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate)
Wavelength selection Double grating monochromator (top/bottom)
Filter cubes (top/bottom)
Detection system Two matched PMTs
Wavelength range Monochromators: 250 - 850 nm
Filters: 200 - 850 nm
Read speed 96 well: 30 seconds
384 well: 1 minute 50 seconds
1536 well: 7 minutes 20 seconds
Light source 100 mW 680 nm laser
Wavelength selection Filter cubes
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 ml, 100 µL with back flush
Plate geometry 6- to 384-well plates, Petri dishes
Dispense precision < 2% at 50-200 µL
Dispense accuracy + 1 µL or 2%
Physical Characteristics
Power 250 Watts max.
Dimensions 15.4" W x 20.7" D x 16.1" H (39 x 52.5 x 41 cm)
Weight 78 lbs (35 kg)
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.