Synergy Neo2 Multi-Mode Reader

Detection > Hybrid Technology Multi-Mode Microplate Readers

Synergy Neo2 Multi-Mode Reader

Overview:

Patented Hybrid OpticsSynergy™ Neo2 Multi-Mode Microplate Reader is designed for speed and ultra high performance, incorporating BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance. Continuously variable bandwidth quadruple monochromators, sensitive high transmission filter-based optics and up to 4 PMTs provide ultra-fast measurements with excellent results. Advanced environment controls, including CO2/O2 control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Barcode-labeled filter cubes help streamline workflows and limit errors, while the available BioStackNeo plate stacker is the fastest on the market - ideal for high throughput requirements. Powerful Gen5™ Data Analysis Software is included, and features designed for 21 CFR Part 11 compliance are available in Gen5 Secure.


BioStack and Neo2
The available BioStack Neo integrates with Synergy Neo2 for fast automated processing of up to 50 plates.


 

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.
HTRF® and the HTRF logo are registered trademarks of Cisbio.


Features:

Fiber-free top optics light path with dual PMT detectors
 
Synergy Neo2 - modular and upgradable

Models:

Ultra Fast Detection Configurations

Part #
NEO2
NEO2 B
NEO2 M
NEO2 MB
NEO2 ALPHA
NEO2 ALPHAB
NEO2 MALPHA
NEO2 MALPHAB
Dual PMT (top) Filter fluorescence & luminescence, FP, TRF, TR-FRET, luminescence
Single monochromator(UV-Vis absorbance)
Quad monochromators (top/bottom variable bandwidth fluorescence, UV-Vis absorbance)  
 
 
 
Single PMT (bottom) measurement(Filter fluorescence & luminescence, FP, TRF, TR-FRET)  
 
 
 

Laser Alpha

 
   
 


Performance & Value Configurations

Part #
NEO2 S
NEO2 SB
NEO2 SM
NEO2 SMB
NEO2 SALPHA
NEO2 SALPHAB
NEO2 SMALPHA
NEO2 SMALPHAB
Single PMT (top): Filter fluorescence & luminescence, FP, TRF, TR-FRET, luminescence
Single monochromator: UV-Vis absorbance
 
 
 
 
Quad monochromators (top/bottom): Variable bandwidth fluorescence, UV-Vis absorbance)  
 
 
 
Single PMT (bottom) measurement(Filter fluorescence & luminescence, FP, TRF, TR-FRET)  
 
 
 

Laser Alpha

 
   
 

 


Specifications:

General
Detection modes UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence, Alpha
Microplate types Monochromator: 1- to 1536-well plates
Filters: 6- to 1536-well plates
Other labware supported Take3™ Micro-Volume Plates
Temperature control 4-Zone incubation to 65 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
CO2 and O2 control Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: + 0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2%at 1% O2
Models for CO2 /O2 or CO2 only are available
Barcode reader 1D and 2D camera-based scanner
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Bandwidth 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed 96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
Fluorescence Intensity
Light source Xenon flash
Sensitivity Filter cubes:
Fluorescein 0.2 pM (384-well low volume plate) - top
Fluorescein 1 pM (1536-well plate) - top
Fluorescein 1 pM (384-well plate) - bottom

Quad monochromator:
Fluorescein 2 pM (384-well low volume plate) - top
Fluorescein 2.5 pM (384-well plate) - bottom
Wavelength selection Quad monochromators (top/bottom)
Filters (top/bottom)
Wavelength range Monochromators: 250 - 850 nm
Filters (dual PMT): 200 - 850 nm
Monochromator bandwidth Variable, from 3 to 50 nm in 1 nm increments
Dynamic range 7 decades
Detector Dual top PMTs
Single top PMT (option)
Low noise PMT (bottom filter system)
Red shifted PMT (top/bottom monochromator system)
Luminescence
Sensitivity 5 amol ATP typical (384-well low volume plate)
Wavelength range 300 - 700 nm
Dynamic range > 6 decades
Fluorescence Polarization
Light source Xenon flash
Detector Dual PMT or single PMT (option)
Sensitivity 1 mP standard deviation at 1 nM fluorescein
(384-well low volume plate)
1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
Wavelength selection Filters
Wavelength range 280 - 850 nm
Time-Resolved Fluorescence
Light source Xenon flash
Sensitivity Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate)
Wavelength selection Quad monochromators (top/bottom)
Filters (top/bottom)
Detector Dual PMT or single PMT (option)
Wavelength range Monos: 250 - 850 nm
Filters (dual PMT): 200 - 850 nm
Alpha
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Read speed 96 well: 30 seconds
384 well: 1 minute 50 seconds
1536 well: 7 minutes 20 seconds
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri dishes
Dead volume 1.1 ml, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy + 1 µL or 2%
Dispense precision < 2% at 50-200 µL
Physical Characteristics
Power 250 Watts max.
Dimensions 15.4" W x 20.7" D x 16.1" H
(39 x 52.5 x 41 cm)
Weight 78 lbs (35 kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.