405 Touch Microplate Washer

Liquid Handling > Microplate Washers

Overview:

The 405 Touch Microplate Washer features the new patented* Verify™ Technology, which runs an automated QC check for manifold tube blockage, and visually reports any failed wells. The patent pending Ultrasonic Advantage™ can then be used to automatically and thoroughly clean the aspirate and dispense manifolds. The 405 Touch, with the unique Verify and Ultrasonic Advantage features, overcomes the common problem of undetected washer manifold clogging which can lead to assay failure. The 405 Touch is the first self-checking, self-maintaining microplate washer available!

The 405™ Touch Microplate Washer incorporates a high resolution touch screen user interface for intuitive and flexible programming of 96- and 384-well microplate wash protocols. The extensive onboard software seamlessly guides users through protocol development, instrument maintenance and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional LHC™ Software.

Cell-based assays and microsphere-based assays are easily run with the 405 Touch’s unique set of available features and modules for gentle washing vacuum filtration and biomagnetic separation. The 405 Touch is Luminex® xMAP® approved; a range of configurations is available for this and a broad variety of other microplate washing applications. Review the models and specifications sections for details on all the possible configurations, there’s certain to be one that fits your laboratory’s requirements.

With its color graphical user interface, fail-safe QC and maintenance tools, and compact, robot-compatible design, the 405 Touch is an easy to use microplate washing solution for a wide range of applications.

* EP 1 637 887

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Features:

Hardware features and options:

Patented built-in Verify sensor automatically checks dispense / aspirate performance and reports clogged locations in the color graphical display.

Washer-Verifier

Accommodates 96- and 384-well microplates
High resolution touch screen user interface
Onboard computer running Microsoft® Windows® CE
Patent pending built-in Ultrasonic Advantage automatically cleans clogged tubes
Two USB flash drive ports enable convenient file transfer, storage and operation
Automated internal 4-buffer switching
Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
All washers vacuum filtration ready (accessory filtration modules sold separately)
All washers biomagnetic separation ready (accessory magnets sold separately)

405 Select TS

96-/384-well washing with patented 96-tube Dual-Action™ manifold
Cell washing is optimized with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

dual-action-manifold

405 HT TS

Fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection:
Superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
96-well washing ready with optional 96-tube Dual-Action manifold

405 TS

405 TS models automate basic 96-well dispensing and aspiration

Software Features:

Context sensitive help system complete with recommendations, diagrams and images is only a key press away when questions arise
Pre-defined protocols and instructional videos walk users through instrument setup and basic use
Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
AutoPrime
Additional software flexibility and 21 CFR Part 11 compliance with Liquid Handling Control™ (LHC) Software for method development, downloading and washing plates.
Run protocols created onboard, downloaded from LHC™ Software or transferred on USB drive

Models:

Part #	96-/384-well	384-well	96-well	Optimized Cell Washing	4-Buffer Switching	Ultrasonic Advantage	Verify Technology	Biomagnetic Separation*	Vacuum Filtration*
405TSHT		•				•		•	•
405TSHTV		•			•	•		•	•
405TSU	•			•				•	•
405TSUV	•			•	•			•	•
405TSUS	•			•		•		•	•
405TSUSQ	•			•	•	•	•	•	•
405TSUVS	•			•	•	•		•	•
405TSUVSQ	•			•	•	•	•	•	•
405TSR			•					•	•
405TSRV			•		•			•	•
405TSRS			•			•		•	•
405TSRSQ			•			•	•	•	•
405TSRVS			•		•	•		•	•
405TSRVSQ			•		•	•	•	•	•

* With available accessory module sold separately

405™ Touch Microplate Washer Configurations
	Feature	Design
HT	Faster 384-well microplate washing	192-tube (16 x 12) manifold Dual-Action™
V	Automated Internal 4-Buffer Switching
S	Ultrasonic Advantage™	Built-in ultrasonic cleaner for manifold self-maintenance
U	96-/384-well microplate washing	96-tube (8 x 12) manifold Dual-Action™
R	96-well microplate washing	96-tube (8 x 12) manifold
Q	Verify™ Technology	Built-in clog detection for dispense and aspirate tubes

Specifications:

General
Assays	ELISA Cell-based assays (model dependent) Magnetic bead, polystyrene bead Multiplex assays Bead-based ELISA Filtration-to-waste processes
Separation	Biomagnetic separation, vacuum filtration
Microplate Types	96- and 384-well (model dependent) Low profile and standard height Solid and filter bottom Filter pore sizes 0.45 µm to 1.2 µm
Shaking	Programmable in minutes and seconds, up to 60 minutes Intensities - slow, medium, fast or variable
Soak Time	Programmable in minutes and seconds, up to 60 minutes
Magnet	High strength 96- and 384-well designs Choice of two designs, custom for BioTek microplate washers: Flat: - Flat-bottom well - beads pulled to band across well bottom - Round-bottom well - beads pulled to button at well bottom Ring: - beads pulled to 4-zone ring at well bottom
Safety/Convenience/Maintenance	Waste and vacuum sensing, fluid and flow detection Aerosol cover Adjustment utility for plate positioning Overflow protection Preprogrammed maintenance routines
Automation	BioStack™ Microplate Stacker - up to 50 ANSI/SBS standard height plates (optional) Except filter bottom plates
Onboard Software	High resolution backlit LED touch screen UI 5.7 inch graphical color display Intel Atom processor running Windows CE Predefined sample wash protocols - ELISA, cell wash, biomagnetic separation and vacuum filtration Quick Menu Create or edit multiple custom protocols Run protocols created onboard, downloaded from LHC™ Software or transferred on USB flash drive
Software	Liquid Handling Control™ (LHC™), for PC wash protocol programming and execution (optional)
Operating Temperature	15° C to 30° C (59° F to 86° F)
Interface	For PC control and BioStack™ integration – 1 USB port, 1 9-pin RS232 port For file transfer – 2 USB Flash Memory ports
Humidity	10% to 85%, non-condensing
Electrical	100 – 240 Volts AC 50/60 Hz, < 8.0 A
Washing
Manifold Types	96-well washing: 96-tube (8 X 12) manifold 96-/384-well washing: Dual-Action™ 96-tube (8 X 12) manifold 384-well washing: Dual-Action™ 192-tube (16 x 12) manifold
Washing Speed	Solid bottom plates: 96-Well: 3 asp. / disp. cycles, 300 µL/well, 96-tube manifold, final aspirate: <30 seconds 384-Well: 3 asp. / disp. cycles, 100 µL/well, 96-tube manifold, final aspirate: <80 seconds 1 asp. / disp. cycle, 400 µL/well, 384 wells, 192-tube manifold, final aspirate: <20 seconds Filter bottom plates: Variable, based on wash parameters
Fluid Delivery	One internal positive displacement pump
Vacuum Filtration	Selectable vacuum levels (approximate finals at 30 seconds): Lowest: -38 mmHg Low: -113 mmHg Medium: -200 mmHg High: -390 mmHg Highest: -506 mmHg Vacuum filtration time range: 5 to 999 seconds
Volume Range	25 to 3,000 µL/well Selectable in 1 µL increments
Buffer Selection	Internal automatic switching for up to 4 wash buffers (optional)
Flow Rates	1 to 11 Includes low flow, cell wash rates (model dependent)
Wash Cycles	1 to 250
Dispense Precision	96-well washing - 96-tube (8 x 12) manifold: 3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 6, absorbance read at 630 nm and 450 nm reference 384-well washing - 192-tube (16 x 12) manifold: < 4% CV: 80 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 7, absorbance read at 630 nm and 450 nm reference
Residual Volume	Solid bottom plates: 96-well washing - 96-tube (8 x 12) manifold: 2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing 384-well washing - 192-tube (16 x 12) manifold: < 2 µL/well: 3-cycle wash, 100 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing Filter bottom plates: 96-well: Average increase weight of plate < 1.2 g after dispensing 300 µL to 0.45 µm plate, filtration 30 seconds, low vacuum, blotted 384-well: Average increase weight of plate < 4.0 g after dispensing 80 µL to 1.2 µm plate, filtration 10 seconds, low vacuum, blotted
Sterilization	Chemical 0.5% sodium hypochlorite, 70% isopropyl alcohol or 70% ethanol solution
Ultrasonic Cleaning	Built-in ultrasonic bath for protein and salt crystal buildup Programmable in hours and minutes, up to 60 minutes (model dependent)
Clog Detection	Built-in Verify technology detects dispense and aspirate tubes for blockage (model dependent)
Supply Bottle Volume	4 L or 10 L (optional)
Physical Characteristics
Dimensions	Depth: 17" (43.2 cm) Width: 14" (35.6 cm) Height: 10" (25.4 cm)
Weight	With internal buffer switching - 36 lbs (16.5 kg) Without - 30 lbs (13.5 kg)
Regulatory
Regulatory	CE and TUV marked. In Vitro Diagnostic use models are available.