405 Touch Microplate Washer

Liquid Handling > Microplate Washers

405 Touch Microplate Washer


The 405 Touch Microplate Washer features the new patented* Verify™ technology, which runs an automated QC check for manifold tube blockage, and visually reports any failed wells.  The patent pending Ultrasonic Advantage™ can then be used to automatically and thoroughly clean the aspirate and dispense manifolds. The 405 Touch, with the unique Verify and Ultrasonic Advantage features, overcomes the common problem of undetected washer manifold clogging which can lead to assay failure. The 405 Touch is the first self-checking, self-maintaining microplate washer available!

The 405™ Touch Microplate Washer incorporates a high resolution touch screen user interface for intuitive and flexible programming of 96- and 384-well microplate wash protocols. The extensive onboard software seamlessly guides users through protocol development, instrument maintenance and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional LHC™ Software.

Cell-based assays and microsphere-based assays are easily run with the 405 Touch’s unique set of available features and modules for gentle washing vacuum filtration and biomagnetic separation. The 405 Touch is Luminex® xMAP® approved; a range of configurations is available for this and a broad variety of other microplate washing applications. Review the models and specifications sections for details on all the possible configurations, there’s certain to be one that fits your laboratory’s requirements.

With its color graphical user interface, fail-safe QC and maintenance tools, and compact, robot-compatible design, the 405 Touch is an easy to use microplate washing solution for a wide range of applications.

* EP 1 637 887

405 Touch

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®



Hardware features and options:


405 Select TS


405 HT TS

405 TS

Software Features:


Part #
Optimized Cell Washing
4-Buffer Switching
Ultrasonic Advantage
Verify™ technology
Biomagnetic Separation*
Vacuum Filtration*


* With available accessory module sold separately


405™ Touch Microplate Washer Configurations
  Feature Design
HT Faster 384-well microplate washing
  • 192-tube (16 x 12) manifold
  • Dual-Action™
V Automated Internal 4-Buffer Switching  
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenance
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
R 96-well microplate washing
  • 96-tube (8 x 12) manifold
Q Verify™ technology
  • Built-in clog detection for dispense and aspirate tubes



Microplate Types 96- and 384-well (model dependent)
Low profile and standard height
Solid and filter bottom
  • Filter pore sizes 0.45 µm to 1.2 µm
Assays ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead
  • Multiplex assays
  • Bead-based ELISA

Filtration-to-waste processes
Separation Biomagnetic separation, vacuum filtration
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Magnet High strength 96- and 384-well designs
Choice of two designs, custom for BioTek microplate washers:
- Flat-bottom well - beads pulled to band across well bottom
- Round-bottom well - beads pulled to button at well bottom
Ring: - beads pulled to 4-zone ring at well bottom
Safety/Convenience/Maintenance Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Preprogrammed maintenance routines
Automation BioStack™ Microplate Stacker - up to 50 ANSI/SBS standard height plates (optional)
  • Except filter bottom plates
Onboard Software High resolution backlit LED touch screen UI
5.7 inch graphical color display
Intel Atom processor running Windows CE
Predefined sample wash protocols - ELISA, cell wash, biomagnetic separation and vacuum filtration
Quick Menu
Create or edit multiple custom protocols
Run protocols created onboard, downloaded from LHC™ Software or transferred on USB flash drive
Software Liquid Handling Control™ (LHC™), for PC wash protocol programming and execution (optional)
Operating Temperature 15° C to 30° C (59° F to 86° F)
Interface For PC control and BioStack™ integration – 1 USB port, 1 9-pin RS232 port
For file transfer – 2 USB Flash Memory ports
Humidity 10% to 85%, non-condensing
Electrical 100 – 240 Volts AC 50/60 Hz, < 8.0 A
Manifold Types 96-well washing:
96-tube (8 X 12) manifold
96-/384-well washing:
Dual-Action™ 96-tube (8 X 12) manifold
384-well washing:
Dual-Action™ 192-tube (16 x 12) manifold
Washing Speed Solid bottom plates:

3 asp. / disp. cycles, 300 µL/well, 96-tube manifold, final aspirate: <30 seconds
3 asp. / disp. cycles, 100 µL/well, 96-tube manifold, final aspirate: <80 seconds
1 asp. / disp. cycle, 400 µL/well, 384 wells, 192-tube manifold, final aspirate: <20 seconds

Filter bottom plates:
Variable, based on wash parameters
Fluid Delivery One internal positive displacement pump
Vacuum Filtration Selectable vacuum levels (approximate finals at 30 seconds):

Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range:
5 to 999 seconds
Volume Range 25 to 3,000 µL/well
Selectable in 1 µL increments
Buffer Selection Internal automatic switching for up to 4 wash buffers (optional)
Flow Rates 1 to 11
Includes low flow, cell wash rates (model dependent)
Wash Cycles 1 to 250
Dispense Precision
  • 96-well washing - 96-tube (8 x 12) manifold:
    3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 6, absorbance read at 630 nm and 450 nm reference
  • 384-well washing - 192-tube (16 x 12) manifold:
    < 4% CV: 80 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 7, absorbance read at 630 nm and 450 nm reference
Residual Volume Solid bottom plates:
  • 96-well washing - 96-tube (8 x 12) manifold:
2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height
optimized prior to testing
  • 384-well washing - 192-tube (16 x 12) manifold:
< 2 µL/well: 3-cycle wash, 100 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing

Filter bottom plates:
  • 96-well:
Average increase weight of plate < 1.2 g after dispensing 300 µL to
0.45 µm plate, filtration 30 seconds, low vacuum, blotted
  • 384-well:
Average increase weight of plate < 4.0 g after dispensing 80 µL to 1.2
µm plate, filtration 10 seconds, low vacuum, blotted
Sterilization Chemical
0.5% sodium hypochlorite, 70% isopropyl alcohol or 70% ethanol solution
Ultrasonic Cleaning Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes
(model dependent)
Clog Detection Built-in Verify™ technology detects dispense and aspirate tubes for blockage (model dependent)
Supply Bottle Volume 4 L or 10 L (optional)
Physical Characteristics
Dimensions Depth: 17" (43.2 cm)
Width: 14" (35.6 cm)
Height: 10" (25.4 cm)
Weight With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.