Gen5 Data Analysis Software

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Gen5 Data Analysis Software integrates with all BioTek imaging and detection systems

Gen5™ Data Analysis Software incorporates more than 30 years of experience and user feedback into an outstanding microplate reader software. Gen5 offers a unique combination of power and ease-of-use that drives productivity and saves time, no matter what application or workflow is incorporated.  Gen5 seamlessly integrates with all BioTek imaging, multi-mode and single mode detection systems, providing control for all reader configurations in a simple setup interface.

Data analysis in Gen5 spans a very wide range of applications, from basic ELISA to high magnification image analysis with an interface designed specifically for all users. Endpoint, kinetic, spectral scanning, well area scanning and well imaging are all available based on the detection system, along with temperature control, reagent injection and stacking operations. Data output is completely configurable, from a quick export to Excel® button for the ultimate fast data transfer, to a completely customizable format for publication quality reporting.

Gen5 is one powerful software for microplate reading and imaging operations.

Subpopulation analysis in Gen5

Subpopulation analysis in Gen5

Kinetic screens

Multiple windows in Gen5 provide the full data picture

Gen5 is available in English, Chinese, Japanese and Russian editions.

Are you a current Gen5 v2 customer looking for the latest Gen5 version? If so, log in to our Customer Resource Center to download the update.

All-User Friendly. Really!

Many software packages claim to be user-friendly, but Gen5's thoughtful design accommodates users at all levels for a wide range of applications, from basic endpoint ELISA quantification to in-depth analysis of high-magnification images. Using Gen5 can be as simple as clicking   Read Now button to start. When the data is returned, you can process, analyze, transform, crunch or otherwise manipulate it using Gen5’s data analysis tools. Basic or complex data is displayed in a clean, colorful way to facilitate data interpretation.

 On the other hand, you can quickly export the data, in fact, Gen5 will even ask

Export Gen5 data

Along with the quick export function, you can use the amazingly customizable reporting and exporting options in Gen5’s Report/Export Builders to provide just the data you want, in the format you want…with publication-ready outputs. Special attention has been placed on result presentation so complex data can be displayed in a clean, colorful way to facilitate data interpretation.

Powerful Functionality

Gen5 includes powerful tools such as 4-P and 5-P curve fits with or without weighting, parallel-line analysis, advanced kinetic analysis, automated pathlength correction, multi-level data transformation, QC features like cut-off and validation criteria definition and calculation... and so much more. Used with Cytation 3 for cell imaging, Gen5 Image+ offers image analysis features typically only seen in dedicated imaging systems. Cell counting, subpopulation analysis and hit-picking processes are readily available .

Web-based Sample Files

A comprehensive searchable library of sample files with data is available, so there’s no need to spend time creating protocols for common (and not so common) applications! New files are added on a regular basis and Gen5 users can upload files to share their experiments and sample data with other Gen5 users.


Note: Gen5 TS is not specifically listed in the chart below as it is used for Epoch™ 2 touchscreen only. Gen5 TS offers the same feature set as standard Gen5 features (features that are applicable only to capability of Epoch 2).

Part #
Gen5 RC
Gen5 Image+
Gen5 Secure
Gen5 Secure Image+
Gen5 IVD
Gen5 IVD Image+
License (Installations per serial number) 2 5 2 25 2 25 2
Reader Control              
End point
Spectral scanning
Linear scanning
Take3 interface
Imaging Features / Analysis
(Cytation 5 & 3)
1.25x – 60x support  
Phase contrast (Cytation 5)  
Digital phase contrast    
Image stitching    
Cell counting  
Subpopulation  analysis    
Imaging and other steps defined for different sections of plate in one procedure  
Data Analysis              
Quantitative analysis  
Qualitative analysis  
Kinetic analysis  
Spectral analysis  
Custom transformations  
Titration, EC50  
Z’ calculation  
Parallel Line analysis  
Security / 21CFR              
User Groups      
Secure database data storage      
Audit Trails      
Electronic Signature      
Single Sign On (SSO) option      
Email Notification      
Levy Jennings QC Trending          
Validation Tools              
Gen5 Validation Package


Minimum Computer System Requirements

Gen5 products will run properly on computer systems that meet or exceed the requirements given below.

For the following Gen5 editions:
Gen5 RC
Gen5 Secure
Gen5 IVD

  • Windows 8.1 Professional Editions, Windows 7 Professional Editions, Windows XP (SP3) (32- and 64-bit versions)
  • Intel® Celeron™ Dual Core Processor T1600 (1.66 GHz, 667 MHz FSB, 1 MB L2 cache) or equivalent
  • 512 MB RAM
  • 2 GB hard drive space
  • Monitor resolution 1024 x 768
  • Keyboard/mouse
  • Microsoft Internet Explorer v 5.0 or later (for online Help)
  • Microsoft Excel 2003 or later for QuickExport and PowerExport (32-bit versions only)
  • Serial or USB port for BioTek instrument connection
  • USB port for software installation


For the following Gen5 editions:

Gen5 Image+
Gen5 Secure Image+
Gen5 IVD Image+

  • Windows 8.1* Professional Editions, Windows 7 Professional Editions (32- and 64-bit versions)
  • Pentium 4 processor(2.0 GHz or higher), equivalent or better
  • 4 GB RAM
  • 1 TB** hard drive space
  • Monitor resolution 1280 x 800
  • Keyboard/mouse
  • Microsoft Internet Explorer v 5.0 or later (for online Help)
  • Microsoft Excel 2003 or later for QuickExport and PowerExport (32-bit versions only)
  • Serial or USB port for BioTek instrument connection
  • USB port for software installation
  • FireWire IEEE 1394b port for imaging (FireWire ports are included with Cytation imaging configurations)
   - For notebook/laptop computers, an Expresscard slot for FireWire port is required.
    - For desktop computers, space must be available for installation of a FireWire port.
(Full height expansion slot with PCIe Express 2.0 x 1 bus)


*Windows 8.1 for Cytation with imaging requires Gen5 v2.06 or higher
** Each image is about 2 MB. Imaging data files can become very large. For best performance and uninterrupted operation, it is recommended that the user implement a robust image file management strategy.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


Product Qualification Packages

5320500 Gen5 Installation Qualification Package
5320502 Gen5 Validation Package