Gen5 Data Analysis Software

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Gen5 Data Analysis Software integrates with all BioTek imaging and detection systems

Gen5 Data Analysis Software integrates with all BioTek imaging and detection systems

Gen5 offers a unique combination of power and ease-of-use that drives productivity and saves time, no matter what application or workflow is incorporated. Data analysis in Gen5 spans a very wide range of applications, from high magnification image analysis to basic ELISA with an interface designed specifically for all users. Data output is completely configurable, from a quick export to Excel® button for the ultimate fast data transfer, to a completely customizable format for publication quality reporting.

Powerful Functionality

Gen5 includes powerful tools such as 4-P and 5-P curve fits with or without weighting, parallel-line analysis, advanced kinetic analysis, automated pathlength correction, multi-level data transformation, QC features like cut-off and validation criteria definition and calculation... and so much more. When preferred, export data using the fast, 1-click Excel export available on most data views.

Gen5 Image+

Image Acquisition and Analysis

Gen5 Image+ provides powerful, intuitive tools for image capture and analysis with Cytation Cell Imaging readers. Manual and automated (experiment-based) image capture interfaces in Gen5 Image+ allow users to quickly find, image, analyze and export data and image parameters in a variety of formats for further study. Used with Cytation for cell imaging, Gen5 Image+ offers image analysis features typically only seen in dedicated imaging systems.

21 CFR Part 11 Compliance

To meet the demands of the GxP laboratory, Gen5 Secure offers features to help laboratories comply with the requirements of  the U.S. FDA's 21 CFR Part 11 Directive.  Flexible multi-user permission levels, protocol and system audit trails and electronic signature are among the important capabilities available for use in a regulated environment.

Built-in and Web-based Sample Files

We’ve done the groundwork for hundreds of protocols in multiple detection modes and imaging – so you don’t have to. Choose from the sample files built in to Gen5, or from, where you’ll find the latest protocols to support the hottest applications for BioTek’s multi-mode and imaging readers.

QC Tools

When the laboratory must work within the guidelines for In-Vitro Diagnostics, Gen5 IVD and  Image+  IVD have QC trending capability, security features and Validation protocols for convenience and efficiency.

Gen5 is available in English, Chinese, Japanese and Russian editions.

Single and multi-mode reading

  • Task Manager  interface simplifies routine operations for everyday use
  • Ready-to-use built in Sample files (protocols and/or experiments), or choose from hundreds online
  • Automated, fast and easy low volume quantification with Take3 and Take3 Trio plates
  • One-touch export to Excel from most data screens
  • Powerful, easily definable data reduction and analysis including relative potency (parallel line analysis), EC/IC50, z’ calculation
  • Multiple curve fits, including 4- and 5-parameter, plus validation and cutoff criteria
  • Endpoint, kinetic, well area scanning, spectral scanning read methods
  • Database or Windows file storage options in Gen5 Secure, Gen5 IVD
  • *.xlsx, *.txt, *.xml file output options


  • Cell counting, subpopulation analysis and hit-picking processes
  • Z-stacking, z-projection, digital phase contrast
  • Montage and image stitching
  • Fluorescence, brightfield, color brightfield and phase contrast image acquisition
  • Import image files from other programs for analysis in Gen5
  • Multi-mode measurement steps and imaging steps combined in single protocol

Note: Gen5 TS is not specifically listed in the chart below as it is used for Epoch™ 2 touchscreen only. Gen5 TS offers the same feature set as standard Gen5 features (features that are applicable only to capability of Epoch 2).

Part #
Gen5 RC
Gen5 Image+
Gen5 Secure
Gen5 Secure Image+
Gen5 IVD
Gen5 IVD Image+
License (Installations per serial number) 2 5 2 25 2 25 2
Reader Control              
End point
Spectral scanning
Linear scanning
Take3 interface
Imaging Features / Analysis
(Cytation 5 & 3)
1.25x – 60x support  
Phase contrast (Cytation 5)  
Digital phase contrast    
Image stitching    
Cell counting  
Subpopulation  analysis    
Imaging and other steps defined for different sections of plate in one procedure  
Data Analysis              
Quantitative analysis  
Qualitative analysis  
Kinetic analysis  
Spectral analysis  
Custom transformations  
Titration, EC50  
Z’ calculation  
Parallel Line analysis  
Security / 21CFR              
User Groups      
Secure database data storage      
Audit Trails      
Electronic Signature      
Single Sign On (SSO) option      
Email Notification      
Levy Jennings QC Trending          
Validation Tools              
Gen5 Validation Package


Minimum Computer System Requirements

Gen5 products will run properly on computer systems that meet or exceed the requirements given below.

For the following Gen5 editions:
Gen5 RC
Gen5 Secure
Gen5 IVD

  • Windows 8.1 Professional Editions, Windows 7 Professional Editions, Windows XP (SP3) (32- and 64-bit versions)
  • Intel® Celeron™ Dual Core Processor T1600 (1.66 GHz, 667 MHz FSB, 1 MB L2 cache) or equivalent
  • 512 MB RAM
  • 2 GB hard drive space
  • Monitor resolution 1024 x 768
  • Keyboard/mouse
  • Microsoft Internet Explorer v 5.0 or later (for online Help)
  • Microsoft Excel 2003 or later for QuickExport and PowerExport (32-bit versions only)
  • Serial or USB port for BioTek instrument connection
  • USB port for software installation


For the following Gen5 editions:

Gen5 Image+
Gen5 Secure Image+
Gen5 IVD Image+

  • Windows 8.1* Professional Editions, Windows 7 Professional Editions (32- and 64-bit versions)
  • Pentium 4 processor(2.0 GHz or higher), equivalent or better
  • 4 GB RAM
  • 1 TB** hard drive space
  • Monitor resolution 1280 x 800
  • Keyboard/mouse
  • Microsoft Internet Explorer v 5.0 or later (for online Help)
  • Microsoft Excel 2003 or later for QuickExport and PowerExport (32-bit versions only)
  • Serial or USB port for BioTek instrument connection
  • USB port for software installation
  • FireWire IEEE 1394b port for imaging (FireWire ports are included with Cytation imaging configurations)
   - For notebook/laptop computers, an Expresscard slot for FireWire port is required.
   - For desktop computers, space must be available for installation of a FireWire port. (Full height or low profile expansion slot with PCIe Express 2.0 X 1 bus)


*Windows 8.1 for Cytation with imaging requires Gen5 v2.06 or higher
** Each image is about 2 MB. Imaging data files can become very large. For best performance and uninterrupted operation, it is recommended that the user implement a robust image file management strategy.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


Product Qualification Packages

5320500 Gen5 Installation Qualification Package
5320502 Gen5 Validation Package