Gen5 Data Analysis Software

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Gen5 Data Analysis Software integrates with all BioTek imaging and detection systems

Gen5™ Data Analysis Software incorporates more than 30 years of experience and user feedback into an outstanding microplate reader software. Gen5 offers a unique combination of power and ease-of-use that drives productivity and saves time, no matter what application or workflow is incorporated.  Gen5 seamlessly integrates with all BioTek imaging, multi-mode and single mode detection systems, providing control for all reader configurations in a simple setup interface.

Data analysis in Gen5 spans a very wide range of applications, from basic ELISA to high magnification image analysis with an interface designed specifically for all users. Endpoint, kinetic, spectral scanning, well area scanning and well imaging are all available based on the detection system, along with temperature control, reagent injection and stacking operations. Data output is completely configurable, from a quick export to Excel® button for the ultimate fast data transfer, to a completely customizable format for publication quality reporting.

Gen5 is one powerful software for microplate reading and imaging operations.

Subpopulation analysis in Gen5

Subpopulation analysis in Gen5

Kinetic screens

Multiple windows in Gen5 provide the full data picture

Gen5 is available in English, Chinese, Japanese and Russian editions.

Are you a current Gen5 v2 customer looking for the latest Gen5 version? If so, log in to our Customer Resource Center to download the update.

All-User Friendly. Really!

Many software packages claim to be user-friendly, but Gen5's thoughtful design accommodates users at all levels for a wide range of applications, from basic endpoint ELISA quantification to in-depth analysis of high-magnification images. Using Gen5 can be as simple as clicking   Read Now button to start. When the data is returned, you can process, analyze, transform, crunch or otherwise manipulate it using Gen5’s data analysis tools. Basic or complex data is displayed in a clean, colorful way to facilitate data interpretation.

 On the other hand, you can quickly export the data, in fact, Gen5 will even ask

Export Gen5 data

Along with the quick export function, you can use the amazingly customizable reporting and exporting options in Gen5’s Report/Export Builders to provide just the data you want, in the format you want…with publication-ready outputs. Special attention has been placed on result presentation so complex data can be displayed in a clean, colorful way to facilitate data interpretation.

Powerful Functionality

Gen5 includes powerful tools such as 4-P and 5-P curve fits with or without weighting, parallel-line analysis, advanced kinetic analysis, automated pathlength correction, multi-level data transformation, QC features like cut-off and validation criteria definition and calculation... and so much more. Used with Cytation 3 for cell imaging, Gen5 Image+ offers image analysis features typically only seen in dedicated imaging systems. Cell counting, subpopulation analysis and hit-picking processes are readily available .

Web-based Sample Files

A comprehensive searchable library of sample files with data is available, so there’s no need to spend time creating protocols for common (and not so common) applications! New files are added on a regular basis and Gen5 users can upload files to share their experiments and sample data with other Gen5 users.


Part #
Gen5 RC
Gen5 Image+
Gen5 Secure
Gen5 Secure Image+
Gen5 IVD
Gen5 IVD Image+
Reader Control              
End point & kinetic
Spectral scanning
Area & linear scanning
Take3 interface
Imaging Features / Analysis
(Cytation 3)
2.5x - 20x  
40x  - 60x  
Cell counting  
Subpopulation analysis  
Data Analysis              
Quantitative & qualitative analysis  
Kinetic analysis  
Spectral analysis  
Custom transformations  
Titration, EC50  
Z’ calculation  
Parallel Line analysis  
Security / 21CFR              
User Groups      
Secure database data storage      
Audit Trails      
Electronic Signature      
Email Notification      
Levy Jennings QC Trending          
Validation Tools              
Gen5 Validation Package


Minimum Requirements

For Gen5 to run successfully, the computer must meet the following requirements:

  • Windows 8.1* Professional Editions, Windows 7 Professional Editions, Windows XP (SP3)**
  • Intel® Celeron™ Dual Core Processor T1600 (1.66 GHz, 667 MHz FSB, 1 MB L2 cache) or equivalent
  • 512 MB RAM or higher/4 GB RAM or higher for imaging
  • 2 GB hard drive space or higher
  • Monitor resolution 1024 x 768 or higher/1280 x 800 or higher for imaging
  • CD-ROM readable drive
  • Keyboard/mouse
  • Microsoft Internet Explorer v 5.0 or higher (for online Help)
  • Microsoft Excel 2003 or later for QuickExport and PowerExport (excluding Excel 2010 64-bit)
  • Serial or USB port for BioTek instrument
  • FireWire IEEE 1394b port for imaging (included with the Cytation 3, if required)

*Windows 8.1 for Cytation 3 with imaging requires Gen5 v2.06 or higher
**Cytation 3 imaging not supported in Windows XP

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


Product Qualification Packages

5320500 Gen5 Installation Qualification Package
5320502 Gen5 Validation Package