Gen5 Microplate Reader and Imager Software

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Gen5 Microplate Reader and Imager Software integrates with all BioTek imaging and detection systems

Gen5 Microplate Reader and Imager Software integrates with all BioTek imaging and detection systems

  Gen5 Image Prime: Advanced image analysis tools

Gen5 Image Prime: Advanced image analysis tools

New Gen5 3.0 Software offers a unique combination of power and ease ­of ­use that drives productivity and saves time, no matter the application or workflow. From high magnification, intracellular image analysis to basic ELISA, Gen5 helps transform raw data and images into meaningful results.

Gather and analyze data from all BioTek microplate readers

Gather and analyze data from all BioTek microplate readers
  • Get a quick start with >150 prepared protocols available in Gen5 and online
  • Endpoint, kinetic, well area scanning, spectral scanning read modes
  • One-button quick export sends data directly to Excel®, no need for multiple steps
  • Predefined Take3 Micro-Volume Plate protocols for direct quantification of 2 µL samples


Explore, image and analyze intra-and inter-cellular activity

Explore, image and analyze intra-and inter-cellular activity
  • Acquire high quality images from 1.25x to 100x with BioTek’s Lionheart FX , up to 60x with Cytation
  • Combine image acquisition steps and multi-mode detection steps to rapidly obtain and analyze phenotypic and quantitative data.
  • Advanced features including image stitching, z-projection and digital phase contrast
  • Import image files from other programs for analysis in Gen5


Imaging: Methods and Processes

Explore, image and analyze intra-and inter-cellular activity
  • Automated cell counting and analysis with image statistics
  • Time-lapse movie making with automated image alignment
  • Montage capture, image stitching
  • Z-stacking and z-projection
  • High speed imaging, image binning for fast kinetics


Advanced Cellular Analysis: Gen5 Image Prime*

Explore, image and analyze intra-and inter-cellular activity
  • Secondary mask for measuring cytoplasm or whole cell
  • Nuclear mask expansion and dilation
  • Custom object - level measurements for translocation analysis and subpopulation analysis


Secure electronic records for GxP and 21 CFR Part 11 compliance

Confirm and protect your results for GxP compliance
  • Built-in system Administration: no additional software required
  • User access control with highly configurable user permissions
  • Protocol and system audit trails with configurable messaging
  • Electronic signature for multiple, definable activities


QC and Validation Tools

QC and Validation Tools
  • Gen5 is fully validated at BioTek. Click here to see the certificate.
  • Gen5 Validation Package provides an efficient step-by-step approach to facilitate your internal validation
  • Compatible with the comprehensive microplate reader IQ/OQ/PQ (Product Qualification Packages) to help speed instrument qualification.
  • Gen5 IVD and Gen5 IVD Image+ have QC trending capability, security features and validation protocols for convenience and efficiency


*Gen5 Image Prime and Gen5 Secure Image Prime are for Research Use Only. Not for Diagnostic Use.

Gen5 is available in English, Chinese, Japanese and Russian editions.

Microplate Reading

Gen5 software controls all BioTek automated microplate readers.  Users can choose from pre-defined common protocols in all detection modes, and provides intuitive data analysis setup

  • Ready-to-use predefined protocols for quick setup and data acquisition
  • Fast and easy low volume quantification with Take3 and Take3 Trio plates
  • One-touch export to Excel from most data screens
  • Powerful data analysis including relative potency, EC/IC50, Z’ calculation
  • Multiple curve fits, including 4- and 5-parameter, plus validation and cutoff criteria
  • Standards and controls from one plate can be applied across multiple plates in a single experiment for  multi-plate workflow efficiency
  • Import endpoint and kinetic date from other applications for analysis in Gen5
  • *.xlsx, *.txt, *.xml file output options


Cell Imaging

Gen5, Gen5 Image+ and Gen5 Image Prime* offer powerful image acquisition and analysis tools for use with Lionheart FX Automated Cell Imager and Cytation Cell Imaging Multi­Mode Readers

  • Cell counting, subpopulation analysis, advanced analysis features in Gen5 Image Prime*
  • Multi-mode and imaging steps combined in single protocol allow hit-picking for imaging efficiency, no need to collect unnecessary data
  • Z-stacking, z-projection, digital phase contrast
  • Montage and image stitching
  • Built-in movie maker for acquired kinetic imaging sequences
  • Fluorescence, brightfield, color brightfield and phase contrast image acquisition
  • Import image files from other programs for analysis in Gen5


GxP Compliance

For regulated laboratory environments, Gen5 Secure offers functionality to help comply with the requirements of 21 CFR Part 11.

  • Electronic signature for data verification, approval, authorization and other customizable events
  • Data, protocol and system audit trails track and record actions with customizable comments
  • Multi-level user permission assignment with highly granular list of protectable functions
  • Multiple groups, with multiple users and permissions are definable
  • User lockout and reset, password aging are all customizable
  • Email notifications for administrator-defined events
  • Single sign-on setup available
  • Multiple administrators can be assigned

*Gen5 Image Prime and Gen5 Secure Image Prime are for Research Use Only. Not for Diagnostic Use.

Gen5 is available in a several editions to meet the requirements of most laboratories. See a detailed feature comparison here.

Gen5
Complete reader control and data analysis for all BioTek microplate readers; basic image analysis with Lionheart FX and Cytation

Gen5RC
Reader Control only, for all BioTek microplate readers

Gen5 Image+
Gen5™ Image+ software offers complete imaging control for Lionheart FX Automated Live Cell Imager and Cytation Cell Imaging Multi¬-Mode Readers. Gen5 Image+ includes image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

Gen5 Secure
Complete reader control and data analysis for all BioTek microplate readers. Gen5 Secure includes features designed to help laboratories maintain critical GxP requirements including 21 CFR Part 11                       

Gen5 Secure Image+
Offers features and functionality found in both Gen5 Image+ and Gen5 Secure

Gen5 IVD
Complete reader control and data analysis for all BioTek microplate readers, with convenient QC Trending module and Validation Package included. Gen5 IVD is ideal for the clinical laboratory

Gen5 IVD Image+
Offers features found in both Gen5 IVD and Gen5 Image+

Gen5 Image Prime*
Gen5™ Image Prime Software offers imaging control for Lionheart FX Automated Live Cell Imager and Cytation Cell Imaging Multi-Mode Readers. Gen5 Image Prime includes image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

Gen5 Secure Image Prime*
Offers features and functionality found in both Gen5 Image Prime and Gen5 Secure.

*Gen5 Image Prime and Gen5 Secure Image Prime are for Research Use Only. Not for Diagnostic Use.

 

General
Recommended Computer System Requirements

For Imaging Applications (Lionheart FX, Cytation 5 and Cytation 3)

  • 64-bit version of Windows 7 or Windows 10 Professional
  • Intel® Core i5 (4th generation) Processor or higher
  • Intel® 8 USB Chipset or higher
  • 8 GB RAM or greater
  • 128 GB Hard Drive space or greater*
  • Monitor resolution of 1600 x 900**
  • Software:
    (Lionheart FX requires Gen5 3.0 or higher)
  • Microsoft Internet Explorer v 9.0 or later (for online Help)
  • Microsoft Excel for Quick Export and PowerExport:
    Gen5 3.0: Excel 2007 (32-bit edition only) or Excel 2010 - 2016 (32- and 64-bit editions) 
    Gen5 2.0: Excel 2007 - 2016 (32-bit editions only)
  • Keyboard and mouse
  • Connectivity:
  • USB 3 port for camera
  • USB 2 or 3 port for instrument

*Each image is approximately 2MB in size. Image file management is the responsibility of the user. For best performance, use an RJ-45 Lan connector for network use. Do not connect to a network using WiFi.
**Gen5 is not designed for use on very high resolution monitors, e.g. 4K or 5K monitors.

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For General Multi-Mode Detection Applications.

  • Windows 10, Windows 8.1, Windows 7 (Professional editions). Gen5 3.0 requires 64-bit OS,
    Gen5 2.09 and lower requires 32- or 64-bit OS.
  • Intel Core i5 or higher
  • 2G RAM or greater
  • 2 GB hard drive space
  • Monitor resolution 1280 x 800** Keyboard/mouse
  • Software: 
  • Microsoft Internet Explorer v 9.0 or later (for online Help)
  • Microsoft Excel for Quick Export and PowerExport:
    Gen5 3.0: Excel 2007 (32-bit edition only) or Excel 2010 - 2016 (32- and 64-bit editions)
    Gen5 2.0: Excel 2007 - 2016 (32-bit editions only)
  • Serial or USB 2.0 or 3.0 port for BioTek instrument connection
  • USB port for software installation
*For Cytation customers who may plan to upgrade to imaging, a 64-bit OS is required for the USB 3.0 camera.
** Gen5 is not designed for use on very high resolution monitors, e.g. 4K or 5K monitors

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

Product Qualification Packages

5320500 Gen5 Installation Qualification Package - IQ
5320502 Gen5 Validation Package - IQ/OQ/PQ

Request Pricing ▶