Synergy MxF Monochromator-Based Fluorescence Microplate Reader

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Quad Monochromator for best signal-to-background in fluorescence

Ultra Fine-Tuned™ technology is what sets the Synergy MxF Fluorescence Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 μm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek's exclusive focus on microplate instrumentation and software make the Synergy MxF the most precise, sensitive and flexible fluorescence microplate reader available today for life-science research applications.

US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Take3 Trio Compatible

  • Fully monochromator-based with quadruple grating architecture for Ultra Fine-Tuned performance.
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • Variable bandpass selection allows to precisely tune the system to a variety of fluorescent dyes.
  • Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
  • Upgradeable architecture allows the purchase additional read modes when requirements change.
  • Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays.

Synergy MxF with top-only fluorescence intensity

Synergy MxF with top and bottom fluorescence intensity

Synergy MxF with top and bottom fluorescence intensity and Dual Reagent Dispenser

Synergy MxF with top-only fluorescence intensity and Dual Reagent Dispenser

Detection mode Fluorescence top and bottom. Upgradeable to UV-Visible Absorbance and Luminescence
Read methods End-point, kinetic, spectral scanning, well-area scanning
Microplate types 1- to 384-well plates and PCR plates
Temperature control 4°C above ambient to 65°C ± 0.5°C at 37°C
Shaking Yes
Software Gen5™ Included
Control through USB or serial port
Automation Yes
Fluorescence Intensity
Sensitivity Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) - top
Fluorescein 5 pM typical (0.5 fmol/well 384-well plate) - bottom
Light source High Energy Xenon Flash
Wavelength selection Double grating monochromators / Top and Bottom
Wavelength range Monos: 250 – 850 nm
Filters: 200 – 700 nm (850 option)
Bandwidth Variable: 9, 13.5, 17, 20 nm
Detection system Dedicated red shifted PMT
Reading speed 96 wells: 11 seconds
384 wells: 22 seconds
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max.
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight < 70 lbs (31.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Standard (Included)


75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Test Plates & Reagents

7092092 Fluorescence Test Plate accessory has picture


Labware Holders

7302030 Cuvette Adapter accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture

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