Synergy MxF Monochromator-Based Fluorescence Microplate Reader
Ultra Fine-Tuned™ technology is what sets the Synergy MxF Fluorescence Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 μm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek's exclusive focus on microplate instrumentation and software make the Synergy MxF the most precise, sensitive and flexible fluorescence microplate reader available today for life-science research applications.
- Fully monochromator-based with quadruple grating architecture for Ultra Fine-Tuned performance.
- Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
- Variable bandpass selection allows to precisely tune the system to a variety of fluorescent dyes.
- Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
- Upgradeable architecture allows the purchase additional read modes when requirements change.
- Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays.
SMT
Synergy MxF with top-only fluorescence intensity
SMTB
Synergy MxF with top and bottom fluorescence intensity
SMTBD
Synergy MxF with top and bottom fluorescence intensity and Dual Reagent Dispenser
SMTD
Synergy MxF with top-only fluorescence intensity and Dual Reagent Dispenser
| General | |
| Detection mode | Fluorescence top and bottom. Upgradeable to UV-Visible Absorbance and Luminescence |
| Read method | End-point, kinetic, spectral scanning, well-area scanning |
| Microplate types | 1- to 384-well plates and PCR plates |
| Temperature control | 4°C above ambient to 65°C ± 0.5°C at 37°C |
| Shaking | Yes |
| Software | Gen5™ Included Control through USB or serial port |
| Automation | Yes |
| Fluorescence Intensity | |
| Sensitivity | Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) - top Fluorescein 5 pM typical (0.5 fmol/well 384-well plate) - bottom |
| Light source | High Energy Xenon Flash |
| Wavelength selection | Double grating monochromators / Top and Bottom |
| Wavelength range | Monos: 250 – 850 nm Filters: 200 – 700 nm (850 option) |
| Bandpass | Variable: 9, 13.5, 17, 20 nm |
| Detection system | Dedicated red shifted PMT |
| Reading speed | 96 wells: 11 seconds 384 wells: 22 seconds |
| Reagent Dispensers | |
| Number | 2 syringe pumps |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dead volume | 1.1 mL, 100 µL with back flush |
| Physical Characteristics | |
| Power | 250 Watts max. |
| Dimensions | 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) |
| Weight | < 70 lbs (31.5 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Standard (Included)

Gen5 Data Analysis Software
Optional

BioStack3 Microplate Stacker
Standard (Included) | ||
Cables | ||
| 75034 | Serial Cable, Modem (9-pin female to 9-pin male) |
|
| 75108 | USB Cable (male A to male B) |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
Test Plates & Reagents | ||
| 7092092 | Fluorescence Test Plate |
|
Optional | ||
Labware Holders | ||
| 7302030 | Cuvette Adapter |
|
Test Plates & Reagents | ||
| 98155 | Sodium Fluorescein Powder (1 mg vial) |
|
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