Synergy Mx Multi-Mode Reader

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Quad Monochromator for best signal-to-background in fluorescence

Variable band pass monochromator for best flexibility

Low volume (2 µL) dsDNA quantification with Take3 plates

Convenient cuvette port for low throughput assays

Ultra Fine-Tuned™ technology is what sets the Synergy Mx Multi-Mode Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 µm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek’s exclusive focus on microplate instrumentation and software make the Synergy Mx the most precise, sensitive and flexible multi-mode microplate reader available today for life-science research applications.

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.

 Take3 Trio Compatible

  • Fully monochromator-based with quadruple grating architecture for Ultra Fine-Tuned performance.
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • High performance fluorescence (top and bottom), absorbance and dedicated luminescence detection systems accommodate all life-science research microplate applications.
  • Variable bandpass selection adds flexibility in fluorescence mode.
  • Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
  • Cuvette port to measure standard 1-cm path cuvettes if only a few samples need to be run, or to compare data with a standard cuvette spectrophotometer.
  • Modular architecture allows purchase of required modes with the ability to upgrade later.
  • Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays or luminescent flash ATP assays.

Synergy Mx is a modular instrument. Read modes and options are available as individual modules at the time of purchase, allowing you to make the best use of your budget. In addition, existing units can be upgraded later when your research requirements expand.

 Synergy™ Mx Model Configurations
 Contact BioTek or use Quote Configurator for part #
 Module  Description
 SM Synergy Mx base. Includes incubation to 65°C and Gen5™ Software.
 A Microplate-based UV-Visible absorbance. Includes xenon flash lamp, dual grating monochromator system, fibers and silicon photodiode detector.
 T Top monochromator-based fluorescence intensity. Includes Xenon Flash lamp, dual grating monochromator system (excitation and emission), top fiber and dedicated red-shifted PMT detector.
 B Bottom monochromator-based fluorescence intensity. Requires top optical system installed. Includes high quality quartz fiber optics and focusing system.
 C Cuvette-based absorbance measurements. Requires “A” module installed. Includes cuvette port and holder, fiber optics and dedicated silicon photodiode detector.
 L Luminescence system. Includes dedicated luminescence optics with low-noise PMT detector.
 D Dual Reagent Dispenser


Detection modes Fluorescence, UV-Visible Absorbance, Luminescence, Time-Resolved Fluorescence (secondary mode)
Read methods End point, kinetic, spectral scanning, well-area scanning
Microplate types 1- to 384-well plates and PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4-Zone™ incubation to 65 °C
Variation+0.2 °C at 37 °C
Shaking linear
Software Gen5™ included
Control through USB or serial port
Automation Yes
Light source High Energy Xenon Flash
Wavelength selection Double Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandwidth 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Reading speed 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Light source High Energy Xenon Flash
Sensitivity Fluorescein 2 pM (0.2 fmol/well 384-well plate) (Top)
Fluorescein 2.5 pM (0.25 fmol/well 384-well plate) (Bottom)
Wavelength selection Double grating monochromators (Top and Bottom)
Wavelength range 250-850 nm
Dynamic range 5 decades
Bandwidth Variable: 9, 13.5, 17, 20 nm
Detection system Dedicated red shifted PMT
Reading speed 96: 11 seconds
384: 22 seconds
Sensitivity 10 amol ATP (flash)
100 amol ATP (glow)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Detection system Low noise PMT
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 70 lbs (31.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Standard (Included)


75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


BioStack Integration Kits

7310047 BioStack Integration Kit for Synergy 2, Synergy Mx, Synergy 4 and Synergy H4 accessory has picture

Labware Holders

7190517S Empty cuvette holder for Synergy Mx (requires C option) accessory has picture
7302030 Cuvette Adapter accessory has picture
7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture


7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

7190554 Synergy Mx Qualification Package

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7092092 Fluorescence Test Plate accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7160006 Glowell Adapter for Synergy Luminescence Tests accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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