Synergy HT Multi-Mode Microplate Reader
Today's research requires that instrumentation be cost effective, reliable and easy to use. In response to those requirements, BioTek Instruments has developed the Synergy™ HT Multi-Mode Microplate Reader. This plate reader for fluorescence, absorbance and luminescence measurements was designed with an emphasis on superior performance in all detection methods. Unlike many multi-mode microplate readers, the Synergy HT utilizes a unique dual-optics design.
When making fluorescence determinations, the Synergy HT uses a tungsten halogen lamp with interference filters for wavelength specificity in conjunction with a PMT detector. The tungsten halogen lamp produces a large amount of light at a constant intensity, providing increased sensitivity and repeatability. Specially designed fluorescent interference filters prevent light leakage from excitation wavelength to the emission wavelength, particularly important with fluorescent compounds with small Stoke's shifts. In addition, the ability to use filters with different bandpasses allows the investigator to tailor their fluorescence measurements to their experiment more effectively.
The low noise PMT is operated in digital photon integration mode for high performance luminescence measurements.
When absorbance measurements are made, the instrument switches to a Xenon Flash Lamp and a monochromator for wavelength selection. The use of a xenon flash lamp allows for both UV and visible light absorbance measurements. The monochromator provides wavelength selection from 200 to 999 nm in 1 nm increments. Thus, dual-optics design allows the investigator to have three different reading modes without compromise.
The Synergy HT has a 4-Zone™ temperature control system that ensures superior temperature uniformity necessary for kinetic assays. With a compact footprint and robot-accessible carrier design, the Synergy HT is also compatible with most automated systems. A Reagent Dispenser option is available. Reader control is via BioTek's Gen5™ Data Analysis Software. USB and serial ports are available for easy connectivity.
US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).
Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.
- Automatic top and bottom epifluorescence read modes optimized for both sensitive homogeneous and cell-based assays
- Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
- Software-controlled automatic probe height adjustment with built-in plate sensor to prevent probe crashes
- Scanning monochromator for wavelength scans to identify peak absorbance and provide full spectral analysis
- Luminescence measurements with top detection for best sensitivity
- Reads 6- to 384-well microplate formats including PCR trays and Terasaki plates
- Well area scanning for popular cell-based assays
- Time-Resolved Fluorescence capability
- Dual Reagent Dispensers
- USB and serial ports for easy connectivity
- Includes Gen5™ Software
| Model | Synergy™ HT | |||
| Part # |
SIAFRT |
SIAFRTD |
SIAFRTCUSTOM |
SIAFRTDCUSTOM |
| Absorbance | • | • | • | • |
| Fluorescence | • | • | • | • |
| Luminescence | • | • | • | • |
| Time Resolved Fluorescence |
• | • | • | • |
| Incubation | • | • | • | • |
| Shaking | • | • | • | • |
| 2 Injectors | • | • | ||
| 3 mm top/5 mm bottom probes |
• | • | ||
| Custom probes | • | • | ||
| Software | Gen5™ included with all configurations | |||
*Possible custom probe configurations are: 3.0 mm top / 3.0 mm bottom. Choice of PMT type (low noise or red extended).
| General | |
| Detection mode | Fluorescence, Time-Resolved Fluorescence (secondary mode), Luminescence, UV-Visible Absorbance |
| Read method | End point, kinetic, spectral scanning, well-area scanning |
| Microplate types | 6- to 384-well plates PCR plates Compatible with Take3 Multi-Volume Plate with 2 µL microspots |
| Temperature control | 4°C above ambient to 50°C; +0.5°C at 37°C |
| Shaking | Yes |
| Software | Gen5™ included Control through USB or serial port |
| Automation | Yes |
| Absorbance | |
| Light source | SQ Xenon Flash |
| Wavelength selection | Monochromator |
| Wavelength range | 200 - 999 nm, 1 nm increment |
| Bandpass | 2.4 nm |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD typical <3% at 3.0 OD typical |
| OD linearity | <1% from 0 to 3.0 OD typical |
| OD repeatability | <0.5% at 2.0 OD typical |
| Stray light | 0.03% at 230 nm typical |
| Reading speed | 96: 14 seconds 384: 26 seconds |
| Fluorescence Intensity | |
| Sensitivity | Fluorescein 5 pM typical (1 fmol/well 96-well plate) (Top and Bottom) |
| Light source | Tungsten Halogen SQ Xenon Flash |
| Wavelength selection | Deep blocking filters |
| Wavelength range | 300 - 700 nm (200 - 850 nm option) |
| Dynamic range | 5 decades |
| Bandpass | Filter dependent |
| Detection system | PMT |
| Reading speed | 96 wells: 31 seconds 384 wells: 80 seconds |
| Luminescence | |
| Sensitivity | 30 amol ATP typical (flash) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Detection system | Low noise PMT |
| Time-Resolved Fluorescence | |
| Light source | SQ Xenon Flash (optional) |
| Wavelength selection | Monochromator |
| Reagent Dispensers | |
| Number | 2 syringe pumps |
| Dispense volume | 5-1000 µL in 1 µL increments |
| Dead volume | 1.1 mL, 100 µL with backflush |
| Physical Characteristics | |
| Power | 100 - 240 VAC 50/60 Hz |
| Dimensions | 16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm) |
| Weight | 40 lbs (18 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Standard (Included)
Gen5 Data Analysis Software
Optional
BioStack3 Microplate Stacker
Standard (Included) | ||
Cables | ||
| 75034 | Serial Cable, Modem (9-pin female to 9-pin male)  |
|
| 75108 | USB Cable (male A to male B) |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
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