Synergy HT Multi-Mode Microplate Reader

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Great flexibility at a great price!

Available Dual Reagent Injector for rapid reagent addition

Low volume (2 µL) dsDNA quantification with Take3 plates

Today's research requires that instrumentation be cost effective, reliable and easy to use. In response to those requirements, BioTek Instruments has developed the Synergy™ HT Multi-Mode Microplate Reader. This plate reader for fluorescence, absorbance and luminescence measurements was designed with an emphasis on superior performance in all detection methods. Unlike many multi-mode microplate readers, the Synergy HT utilizes a unique dual-optics design.

When making fluorescence determinations, the Synergy HT uses a tungsten halogen lamp with interference filters for wavelength specificity in conjunction with a PMT detector. The tungsten halogen lamp produces a large amount of light at a constant intensity, providing increased sensitivity and repeatability. Specially designed fluorescent interference filters prevent light leakage from excitation wavelength to the emission wavelength, particularly important with fluorescent compounds with small Stoke's shifts. In addition, the ability to use filters with different bandpasses allows the investigator to tailor their fluorescence measurements to their experiment more effectively.

The low noise PMT is operated in digital photon integration mode for high performance luminescence measurements.

When absorbance measurements are made, the instrument switches to a Xenon Flash Lamp and a monochromator for wavelength selection. The use of a xenon flash lamp allows for both UV and visible light absorbance measurements. The monochromator provides wavelength selection from 200 to 999 nm in 1 nm increments. Thus, dual-optics design allows the investigator to have three different reading modes without compromise.

The Synergy HT has a 4-Zone™ temperature control system that ensures superior temperature uniformity necessary for kinetic assays. With a compact footprint and robot-accessible carrier design, the Synergy HT is also compatible with most automated systems. A Reagent Dispenser option is available. Reader control is via BioTek's Gen5™ Data Analysis Software. USB and serial ports are available for easy connectivity.

 
US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.

 Take3 Trio Compatible

  • Automatic top and bottom epifluorescence read modes optimized for both sensitive homogeneous and cell-based assays
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • Software-controlled automatic probe height adjustment with built-in plate sensor to prevent probe crashes
  • Scanning monochromator for wavelength scans to identify peak absorbance and provide full spectral analysis
  • Luminescence measurements with top detection for best sensitivity
  • Reads 6- to 384-well microplate formats including PCR trays and Terasaki plates
  • Well area scanning for popular cell-based assays
  • Time-Resolved Fluorescence capability
  • Dual Reagent Dispensers
  • USB and serial ports for easy connectivity
  • Includes Gen5™ Software
 Part #

SIAFRT

SIAFRTD

 Absorbance
 Fluorescence
 Luminescence
 Time Resolved
 Fluorescence
 Incubation
 Shaking
 2 Injectors  
 3 mm top/5 mm
 bottom probes
 Software Gen5™ included with all configurations

 

General
Detection mode Fluorescence, Time-Resolved Fluorescence (secondary mode), Luminescence, UV-Visible Absorbance
Read method End point, kinetic, spectral scanning, well-area scanning
Microplate types 6- to 384-well plates
PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4°C above ambient to 50°C; +0.5°C at 37°C
Shaking Yes
Software Gen5™ included
Control through USB or serial port
Automation Yes
Absorbance
Light source SQ Xenon Flash
Wavelength selection Monochromator
Wavelength range 200 - 999 nm, 1 nm increment
Bandpass 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD typical
<3% at 3.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 14 seconds
384: 26 seconds
Fluorescence Intensity
Sensitivity Fluorescein 5 pM typical (1 fmol/well 96-well plate) (Top and Bottom)
Light source Tungsten Halogen
SQ Xenon Flash
Wavelength selection Deep blocking filters
Wavelength range 300 - 700 nm (200 - 850 nm option)
Dynamic range 5 decades
Bandpass Filter dependent
Detection system PMT
Reading speed 96 wells: 31 seconds
384 wells: 80 seconds
Luminescence
Sensitivity 30 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Detection system Low noise PMT
Time-Resolved Fluorescence
Light source SQ Xenon Flash (optional)
Wavelength selection Monochromator
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5-1000 µL in 1 µL increments
Dead volume 1.1 mL, 100 µL with backflush
Physical Characteristics
Power 100 - 240 VAC 50/60 Hz
Dimensions 16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm)
Weight 40 lbs (18 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Standard (Included)

Cables

75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310046 BioStack Integration Kit for Synergy HT accessory has picture

Bottles & Tubing

7112186 3 feet (1 meter) tubing for Synergy dispenser box

Cables

75053 Serial Cable, Null Modem (9-pin female to 25-pin female) accessory has picture

Filter Accessories

7080541 Empty Filter Wheel for Synergys and FLx800 accessory has picture
7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filters

7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092205 325/50 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7092159 485/10 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture
7082210 545/40 nm Fluorescence Filter accessory has picture
7092170 550/10 nm Fluorescence Filter accessory has picture
7092171 556/10 nm Fluorescence Filter accessory has picture
7092172 560/10 nm Fluorescence Filter accessory has picture
7082215 560/15 nm Fluorescence Filter accessory has picture
7082211 560/20 nm Fluorescence Filter accessory has picture
7082212 560/40 nm Fluorescence Filter accessory has picture
7082271 565/10 nm Fluorescence Filter
7092173 570/10 nm Fluorescence Filter accessory has picture
7082264 570/100 nm Fluorescence Filter accessory has picture
7092174 575/10 nm Fluorescence Filter accessory has picture
7082245 575/15 nm Fluorescence Filter accessory has picture
7092175 580/10 nm Fluorescence Filter accessory has picture
7082244 580/50 nm Fluorescence Filter accessory has picture
7092176 585/10 nm Fluorescence Filter accessory has picture
7092177 590/10 nm Fluorescence Filter accessory has picture
7082225 590/20 nm Fluorescence Filter accessory has picture
7082224 590/35 nm Fluorescence Filter accessory has picture
7092178 596/15 nm Fluorescence Filter accessory has picture
7082252 600/40 nm Fluorescence Filter accessory has picture
7092179 610/10 nm Fluorescence Filter accessory has picture
7082251 620/15 nm Fluorescence Filter accessory has picture
7082213 620/40 nm Fluorescence Filter accessory has picture
7092180 625/10 nm Fluorescence Filter accessory has picture
7092181 635/20 nm Fluorescence Filter accessory has picture
7082214 635/32 nm Fluorescence Filter accessory has picture
7092182 645/10 nm Fluorescence Filter accessory has picture
7082257 645/15 nm Fluorescence Filter accessory has picture
7082226 645/40 nm Fluorescence Filter accessory has picture
7092183 660/10 nm Fluorescence Filter accessory has picture
7092184 665/34 nm Fluorescence Filter accessory has picture
7092185 670/10 nm Fluorescence Filter accessory has picture
7092186 670/20 nm Fluorescence Filter accessory has picture
7092187 680/10 nm Fluorescence Filter accessory has picture
7092188 680/20 nm Fluorescence Filter accessory has picture
7082229 680/30 nm Fluorescence Filter accessory has picture
7092189 690/10 nm Fluorescence Filter accessory has picture
7092190 700/20 nm Fluorescence Filter accessory has picture
7092191 710/20 nm Fluorescence Filter accessory has picture
7092192 730/40 nm Fluorescence Filter accessory has picture
7092193 760/20 nm Fluorescence Filter accessory has picture
7092194 760/34 nm Fluorescence Filter accessory has picture
7092196 785/20 nm Fluorescence Filter accessory has picture
7092197 807/25 nm Fluorescence Filter accessory has picture
7092198 818/40 nm Fluorescence Filter accessory has picture

Labware Holders

7302030 Cuvette Adapter accessory has picture
7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture

Miscellaneous

7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

7090521 Synergy HT Qualification Package

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7092092 Fluorescence Test Plate accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7260522E Absorbance Test Plate accessory has picture

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