BioTek: Microplate Instrumentation & Software Solutions
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Synergy H4 Hybrid Multi-Mode Microplate Reader

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Patented Hybrid optics: sensitivity of filters, flexibility of monochromators


Variable band pass monochromator for best flexibility


Low volume (2 µL) dsDNA quantification with Take3 plates

Synergy™ H4 with Hybrid Technology™ is a patented multi-mode detector that combines the optical systems of Synergy Mx and Synergy 2 in one compact instrument. It is the ideal instrument for research and drug discovery applications when having to choose between flexibility and performance is not an option. The monochromator-based optics provides a high level of flexibility; any wavelength can be used from the low UV to the near infrared. The quadruple grating optical system, equipped with a variable bandpass selection system, is ideal for spectral scanning applications. The filter-based optics use dichroic mirrors for enhanced performance. This system is faster and more sensitive than the monochromator optics. A dual reagent dispenser option is available for inject and read assays such as flash luminescent assays and fluorescent ion channel assays. Additional read modes include: fluorescence polarization, time resolved fluorescence and AlphaScreen/AlphaLISA.

Watch this interview at SelectScience.tv with Marina Bruss from Analytica 2010 to learn more about the Synergy H4 Hybrid Multi-Mode Microplate Reader.



Hybrid Technology™ protected under U.S. Patent 8,218,141.

HTRF® and the HTRF logo are registered trademarks of Cisbio.
Transcreener® is a registered trademark of BellBrook Labs.
The LanthaScreen™ and GeneBLAzer® technologies from Invitrogen have been validated on BioTek instruments.

 

 Take3 Trio Compatible       Take3 Trio Compatible

  • Hybrid Technology: High-performance filter-based detection, flexible monochromator-based detection.
  • Detection Modes: Fluorescence intensity (FI), Fluorescence Polarization (FP), Time- Resolved Fluorescence (TRF), TR-FRET, High-Performance Luminescence, UV-Visible Absorbance, AlphaScreen / AlphaLISA.
  • Compatible with Take3™ Micro-Volume Plate with 2 μL microspots for low volume assays

Take3 Micro-Volume Plate

  • Quadruple monochromator system and variable bandpass selection: Synergy H4 optics incorporates two double-grating monochromators. This quadruple design provides the best in spectral scanning performance and flexibility. Variable bandpass selection system adds flexibility.

  • Deep blocking filters and dichroic mirrors: Synergy H4’s filter/dichroic combination provides the best possible performance for fluorescence, time resolved fluorescence, fluorescence polarization and AlphaScreen / AlphaLISA applications.

Synergy™ H4 is BioTek's second generation Hybrid reader. Like Synergy 4, it offers the combined benefits of filter-based and monochromator-based detection.

 Synergy™ H4
Part # Absorbance LUM FI (Filters & Monos) FP TRF Injectors Software
H4MF           Gen5™ included
H4MFD        
H4MFA        
H4MFAD      
H4MLF        
H4MLFD      
H4MLFA      
H4MLFAD    
H4MLFPTA  
H4MLFPTAD
H4MFPT      
H4MFPTD    
H4MLFPT    
H4MLFPTD  
H4MFPTA    
H4MFPTAD  

 

General
Wavelength Selection Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141)
Quadruple Monochromators and Filters / Dichroics
Detection mode Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance
Read method End point, kinetic, spectral scanning, well-area scanning
Microplate types Monochromator system 1- to 384-well plates
Filter system 1- to 1536-well plates
PCR plates
Compatible with Take3™ Multi-Volume Plate with 2 µL microspots
Temperature control 4° C above ambient to 65°, C + 0.5° C at 37° C
Shaking Yes
Software Gen5™ included; Control through USB or serial port
Automation Yes
AlphaScreen/AlphaLISA
Light source Tungsten Halogen Lamp
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µ­L/well in 384-well plate
Dynamic range >6 decades
Reading speed 2 minutes typical for 96-well plate
Detection system Ultra Low Noise PMT
Absorbance
Light source Xenon Flash Lamp
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD typical <3% at 3.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 11 seconds
384: 22 seconds
Fluorescence Intensity
Sensitivity Monochromators:
Fluorescein 2 pM typical (0.2 fmol/well; 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well; 384-well plate) (Bottom)
Filters/mirrors:
Fluorescein 1 pM typical (0.1 fmol/well; 384-well plate)
Light source Tungsten Halogen Lamp
High Energy Xenon Flash Lamp
Wavelength selection Double grating monochromators (Top/Bottom) and,
Deep blocking filters / dichroic mirrors (Top)
Wavelength range Monochromators: 250 - 850 nm
Filters: 200 - 700 nm (850 nm option)
Dynamic range Monochromators: 5 decades
Filters/mirrors: >6 decades
Bandpass Monochromators: variable 9, 13.5, 17, 20 nm
Filters: filter-dependant, from 5 nm to >100 nm
Detection system Red shifted PMT for monochromator system
Low Noise PMT for filter-based system
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Luminescence
Sensitivity 10 amol ATP typical (flash)
100 amol ATP (glow) typical
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Fluorescence Polarization
Light source Tungsten Halogen
Sensitivity 3 mP at 1 nM fluorescein typical
Wavelength selection Deep blocking filters / dichroic mirrors (Top)
Wavelength range 400 - 700 nm (320 - 850 nm option)
Time-Resolved Fluorescence
Light source High Energy Xenon Flash Lamp
Sensitivity Europium 60 fM typical with filters (6 amol/well in 384-well plate)
Wavelength selection Deep blocking filters / dichroic mirrors (Top)
Double grating monochromator (Top / Bottom)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Monochromators: 250 - 850 nm
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 78 lbs (35 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Optional

BioStack Integration Kits

7310047 BioStack Integration Kit for Synergy 2, Synergy Mx, Synergy 4 and Synergy H4 accessory has picture

Bottles & Tubing

7112186 3 feet (1 meter) tubing for Synergy dispenser box

Filter Accessories

7080541 Empty Filter Wheel for Synergys and FLx800 accessory has picture
7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filters

7092202 274/5 nm Fluorescence Filter accessory has picture
7092146 276/10 nm Fluorescence Filter accessory has picture
7092145 276/5 nm Fluorescence Filter accessory has picture
7092148 280/10 nm Fluorescence Filter accessory has picture
7092147 280/5 nm Fluorescence Filter accessory has picture
7082259 284/10 nm Fluorescence Filter accessory has picture
7092149 284/5 nm Fluorescence Filter accessory has picture
7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092205 325/50 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7092151 355/20 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture
7082210 545/40 nm Fluorescence Filter accessory has picture
7092170 550/10 nm Fluorescence Filter accessory has picture
7092171 556/10 nm Fluorescence Filter accessory has picture
7092172 560/10 nm Fluorescence Filter accessory has picture
7082215 560/15 nm Fluorescence Filter accessory has picture
7082211 560/20 nm Fluorescence Filter accessory has picture
7082212 560/40 nm Fluorescence Filter accessory has picture
7082271 565/10 nm Fluorescence Filter
7092173 570/10 nm Fluorescence Filter accessory has picture
7082264 570/100 nm Fluorescence Filter accessory has picture
7092174 575/10 nm Fluorescence Filter accessory has picture
7082245 575/15 nm Fluorescence Filter accessory has picture
7092175 580/10 nm Fluorescence Filter accessory has picture
7082244 580/50 nm Fluorescence Filter accessory has picture
7092176 585/10 nm Fluorescence Filter accessory has picture
7092177 590/10 nm Fluorescence Filter accessory has picture
7082225 590/20 nm Fluorescence Filter accessory has picture
7082224 590/35 nm Fluorescence Filter accessory has picture
7092178 596/15 nm Fluorescence Filter accessory has picture
7082252 600/40 nm Fluorescence Filter accessory has picture
7092179 610/10 nm Fluorescence Filter accessory has picture
7082265 620/10 nm Fluorescence Filter accessory has picture
7082251 620/15 nm Fluorescence Filter accessory has picture
7082213 620/40 nm Fluorescence Filter accessory has picture
7092180 625/10 nm Fluorescence Filter accessory has picture
7092181 635/20 nm Fluorescence Filter accessory has picture
7082214 635/32 nm Fluorescence Filter accessory has picture
7092182 645/10 nm Fluorescence Filter accessory has picture
7082226 645/40 nm Fluorescence Filter accessory has picture
7092183 660/10 nm Fluorescence Filter accessory has picture
7092184 665/34 nm Fluorescence Filter accessory has picture
7082266 665/8 nm Fluorescence Filter accessory has picture
7092185 670/10 nm Fluorescence Filter accessory has picture
7092186 670/20 nm Fluorescence Filter accessory has picture
7092187 680/10 nm Fluorescence Filter accessory has picture
7092188 680/20 nm Fluorescence Filter accessory has picture
7082229 680/30 nm Fluorescence Filter accessory has picture
7092189 690/10 nm Fluorescence Filter accessory has picture
7092190 700/20 nm Fluorescence Filter accessory has picture
7092191 710/20 nm Fluorescence Filter accessory has picture
7092192 730/40 nm Fluorescence Filter accessory has picture
7092193 760/20 nm Fluorescence Filter accessory has picture
7092194 760/34 nm Fluorescence Filter accessory has picture
7092195 781/20 nm Fluorescence Filter accessory has picture
7092196 785/20 nm Fluorescence Filter accessory has picture
7092197 807/25 nm Fluorescence Filter accessory has picture
7092198 818/40 nm Fluorescence Filter accessory has picture

Labware Holders

7302030 Cuvette Adapter accessory has picture
7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture

Mirrors

7132121 Half-size dichroic mirror, 50%, Ex 200-850 nm, Em 200-850 nm accessory has picture
7137400 Full-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7137435 Full-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7137455 Full-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7137510 Full-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7137525 Full-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7137545 Full-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7137550 Full-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7137555 Full-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7137570 Full-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7137595 Full-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7137660 Full-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture
7138320 Half-Size Dichroic Mirror, Ex. 260-305, Em. 335-750 accessory has picture
7138365 Half-Size Dichroic Mirror, Ex. 290-350, Em. 380-800 accessory has picture
7138400 Half-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7138435 Half-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7138455 Half-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7138510 Half-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7138525 Half-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7138545 Half-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7138550 Half-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7138555 Half-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7138570 Half-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7138595 Half-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7138660 Half-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture
7139635 Half-Size Dichroic Mirror, Ex. 640-780, Em. 400-630 accessory has picture
8030515S Empty Mirror Holder accessory has picture
8030516S FP Compatible Empty Mirror Holder accessory has picture

Miscellaneous

7132166 Optics Accessory Case accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

8030514 Synergy H4 Qualification Package

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3Trio Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7092092 Fluorescence Test Plate accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7160006 Glowell Adapter for Synergy Luminescence Tests accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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