Synergy H1m Monochromator-Based Multi-Mode Microplate Reader

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Cost-effective fully monochromator-based reader

Synergy™ H1m is the most cost-effective monochromator-based multi-mode microplate reader on the market. Its monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories. Synergy H1m can be turned into a high performance Synergy H1 Hybrid Multi-Mode Microplate Reader with the addition of a filter-based optical module. A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).

To create the ideal physiological environment for live-cell assays, the new Gas Controller for Synergy H1m allows control and monitoring of CO2 and O2 levels within the Synergy H1. The Gas Controller, along with user-adjustable orbital shaking and advanced 4-ZoneTM temperature control make up the CellControlTM feature set available for the Synergy H1m readers.

US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.

Take3 Trio Compatible

  • Fully monochromator-based with quadruple grating architecture
  • Upgradeable to patented Hybrid monochromator-filter optical system
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • High performance fluorescence (top and bottom), absorbance and luminescence detection systems accommodate all life-science research microplate applications.
  • Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
  • Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays or luminescent flash ATP assays.
  • Comes with Gen5 software: reader control, advanced data analysis and flexible Excel export in one software package.

Part #


Monochromator fluorescence


Monochromator absorbance




Time-Resolved Fluorescence

• *
• *
• *

Dual reagent dispenser



Temperature control to 45°C

Gen5 data analysis software

Gas Controller Ready Models **


* Secondary mode

** These configurations have the same features as the non- Gas Controller Ready configurations in the chart above. Gas Controller sold separately.


Wavelength Selection Quadruple Monochromators
Detection mode Fluorescence, Luminescence, UV-Visible Absorbance, TRF (secondary mode)
Read methods End-point, kinetic, spectral scanning, well-area scanning
Microplate types 1- to 384-well plates
Compatible with Take3 plate with 2 µL microspots
Maximum Plate Height Absorbance: 0.8" (20.3 mm)
Fluorescence & Luminescence: 0.89" (22.6 mm)
Temperature control 4-Zone™ incubation to 45 °C
Variation+0.2 °C at 37 °C
Shaking linear, orbital, double orbital
Software Gen5™ reader control, advanced data analysis, Excel export
Control through USB or serial port
Automation Yes
Light source Xenon Flash Lamp
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed 96: 11 seconds
384: 22 seconds
Fluorescence Intensity
Sensitivity Fluorescein 2.5 pM (0.25 fmol/well 384-well plate)
Light source Xenon Flash Lamp
Wavelength selection Double grating monochromators (Top and Bottom)
Wavelength range 250 - 700 nm (850 nm option)
Dynamic range 5 decades
Detection system Low noise PMT
Reading speed 96: 11 seconds
384: 22 seconds
Sensitivity 20 amol ATP (flash)
100 amol ATP (glow)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Reagent Dispensers
Dispense Precision <2% at 50-200 µL
Dispense Accuracy +1 µL or 2%
Number 2 syringe pumps
Plate Geometry 1- to 384-well microplates
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1 mL, 100 µL with back flush
Physical Characteristics
Power 130 Watts max
Dimensions 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm)
Weight 50 lbs (22.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Standard (Included)


75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


Labware Holders

7302030 Cuvette Adapter accessory has picture
7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture


7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7160006 Glowell Adapter for Synergy Luminescence Tests accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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