Synergy H1 Hybrid Multi-Mode Microplate Reader

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Cost-effective Hybrid: value, sensitivity, flexibility

Low volume (2 µL) dsDNA quantification with Take3 plates

Live-cell assays with temperature and CO2/O2 control

Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader that can be turned into a high-performance patented Hybrid system with the addition of a filter-based optical module. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories.

The filter module is a completely independent add-on that includes its own light source, and a high performance dichroic-based wavelength selection system. With its very high optical efficiency, this module supports advanced detection modes such as Fluorescence Polarization, Time-Resolved Fluorescence & TR-FRET and filtered luminescence (e.g. BRET). A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).

To create the ideal physiological environment for live-cell assays, the new Gas Controller for Synergy H1 allows control and monitoring of CO2 and O2 levels within the Synergy H1. The Gas Controller, along with user-adjustable orbital shaking and advanced 4-ZoneTM temperature control make up the CellControlTM feature set available for the Synergy H1 readers.

The Versatility of Synergy H1

 
US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.

HTRF® and the HTRF logo are registered trademarks of Cisbio.

Take3 Trio Compatible

  • Patented Hybrid Technology™ combines flexible monochromator detection with high performance dichroic-based detection.
  • Compatible with the Gas Controller for control and monitoring of CO2 and O2.
  • Compatible with Take3™ Micro-Volume Plate: samples down to 2 µL volume can be measured. Especially useful when working with precious samples, for fast and accurate DNA/RNA quantification at 260 nm
Take3 Micro-Volume Plate
  • Quadruple grating monochromator for maximum flexibility and ease of use.
  • Dichroic-based filter optics, for best performance and advanced detection technologies such as fluorescence polarization and time resolved fluorescence.
  • Comes with Gen5 software: reader control, advanced data analysis and flexible Excel export in one software package.
Part #
H1M
H1MD
H1F
H1FD
H1MF
H1MFD
Monochromator fluorescence
 
 
Monochromator absorbance
 
 
Full-light luminescence
Filter/dichroic fluorescence  
 
Fluorescence polarization    
Time resolved fluorescence
• *
• *
Filtered luminescence    
Dual reagent dispenser  
 
 
Temperature control to 45°C
Gen5 data analysis software
* Secondary mode            
             
Gas Controller Compatible Configurations** H1MG H1MDG H1FG H1FDG H1FMG H1MFDG
** These configurations have the same features as the non- Gas Controller Compatible configurations in the chart above. Gas Controller sold separately.

 

 

General
Wavelength Selection Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141)
Quadruple Monochromators and Filters / Dichroics
Detection mode Monochromator system: Fluorescence, Luminescence, UV-Visible Absorbance
Filter system: Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence
Read methods End point, kinetic, spectral scanning, well-area scanning
Microplate types 1- to 384-well plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4-Zone™ incubation to 45 °C
Variation+0.2 °C at 37 °C
Shaking linear, orbital, double orbital
Software Gen5™ reader control included, advanced data analysis, Microsoft® Excel® export. Control through USB or serial port
Automation Yes
CO2 and O2 Control Gas Controller option available for Synergy H1 models
Absorbance
Light source Xenon Flash Lamp
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy ±2 nm
Monochromator wavelength repeatability ±0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed 96: 11 seconds
384: 22 seconds
Fluorescence Intensity
Sensitivity Monochromators:
Top: fluorescein 2.5 pM (0.25 fmol/well 384-well plate)
Bottom: fluorescein 4 pM (0.4 fmol/well 384-well plate)
Filters/mirrors:
Fluorescein 0.25 pM (0.025 fmol/well 384-well plate)
Light source Xenon Flash Lamp
Wavelength selection Double grating monochromators (Top and Bottom) and,
Deep blocking bandpass filters / dichroic mirrors (Top)
Wavelength range Monos: 250 - 700 nm (850 option)
Filters: 200 - 700 nm (850 option)
Dynamic range 5 decades
Detection system Two PMT detectors: one for monochromator system, one for filter system
Reading speed 96: 11 seconds
384: 22 seconds
Luminescence
Sensitivity Monochromator system: 20 amol ATP (flash)
Filter system: 10 amol ATP (flash)
100 amol ATP (glow)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Fluorescence Polarization
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Wavelength range 320 - 700 nm (850 nm option)
Time-Resolved Fluorescence
Light source Xenon Flash Lamp
Sensitivity Europium 40 fM with filters (4 amol/well in 384-well plate)
Europium 1200 fM with monos (120 amol/well in 384-well plate)
Wavelength range Monos: 250 - 850 nm
Filters: 200 - 700 nm (850 nm option)
Reagent Dispensers
Dispense Precision <2% at 50-200 µL
Dispense Accuracy ±1 µL or 2%
Number 2 syringe pumps
Plate Geometry 1- to 384-well microplates
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1 mL, 100 µL with back flush
Physical Characteristics
Power 130 Watts max
Dimensions 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm)
Weight 50 lbs (22.5 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available. RoHS Compliant.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Optional

BioStack Integration Kits

7310045 BioStack Integration Kit for Synergy H1

Bottles & Tubing

1210011 Calibration Kit for CO2 Controller

Filter Accessories

7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filter Cubes

8040501S Filter Cube: Blue (360/460) / Green (485/528) accessory has picture
8040502 Filter Cube: Green (485/528) / Red (530 / 590) accessory has picture
8040505S Filter Cube: HTRF® – TR-FRET accessory has picture
8040506 Filter Cube: LanthaScreen – TR-FRET accessory has picture
8040507 Filter Cube: Lance – TR-FRET accessory has picture
8040553S Filter Cube – Luminescence accessory has picture
8040561 Filter Cube: Green FP (485/528) accessory has picture
8040562 Filter Cube: Red FP (530 / 590) accessory has picture
8040563 Blue/UV FP filter set: 360/40 EX, 460/40 EM, 400 nm mirror, UV polarizers
8040564 Empty Filter Cube - Fluorescence Polarization Visible (excitation > 400nm)
8040565 Empty Filter Cube - Fluorescence Polarization UV (excitation between 300 and 400 nm)
8040566 Empty Filter Cube - non FP applications
8040574 BRET 1 cube, 460/40 and 540/25 nm
8040575 BRET 2 cube, 410/80 nm and 515/30 nm
8040576 Filter Block Asby 440 Blue/Green

Filters

7092202 274/5 nm Fluorescence Filter accessory has picture
7092146 276/10 nm Fluorescence Filter accessory has picture
7092145 276/5 nm Fluorescence Filter accessory has picture
7092148 280/10 nm Fluorescence Filter accessory has picture
7092147 280/5 nm Fluorescence Filter accessory has picture
7082259 284/10 nm Fluorescence Filter accessory has picture
7092149 284/5 nm Fluorescence Filter accessory has picture
7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092205 325/50 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7092151 355/20 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture
7082210 545/40 nm Fluorescence Filter accessory has picture
7092170 550/10 nm Fluorescence Filter accessory has picture
7092171 556/10 nm Fluorescence Filter accessory has picture
7092172 560/10 nm Fluorescence Filter accessory has picture
7082215 560/15 nm Fluorescence Filter accessory has picture
7082211 560/20 nm Fluorescence Filter accessory has picture
7082212 560/40 nm Fluorescence Filter accessory has picture
7082271 565/10 nm Fluorescence Filter
7092173 570/10 nm Fluorescence Filter accessory has picture
7082264 570/100 nm Fluorescence Filter accessory has picture
7092174 575/10 nm Fluorescence Filter accessory has picture
7082245 575/15 nm Fluorescence Filter accessory has picture
7092175 580/10 nm Fluorescence Filter accessory has picture
7082244 580/50 nm Fluorescence Filter accessory has picture
7092176 585/10 nm Fluorescence Filter accessory has picture
7092177 590/10 nm Fluorescence Filter accessory has picture
7082225 590/20 nm Fluorescence Filter accessory has picture
7082224 590/35 nm Fluorescence Filter accessory has picture
7092178 596/15 nm Fluorescence Filter accessory has picture
7082252 600/40 nm Fluorescence Filter accessory has picture
7092179 610/10 nm Fluorescence Filter accessory has picture
7082265 620/10 nm Fluorescence Filter accessory has picture
7082251 620/15 nm Fluorescence Filter accessory has picture
7082213 620/40 nm Fluorescence Filter accessory has picture
7092180 625/10 nm Fluorescence Filter accessory has picture
7082214 635/32 nm Fluorescence Filter accessory has picture
7092182 645/10 nm Fluorescence Filter accessory has picture
7082226 645/40 nm Fluorescence Filter accessory has picture
7092183 660/10 nm Fluorescence Filter accessory has picture
7092184 665/34 nm Fluorescence Filter accessory has picture
7082266 665/8 nm Fluorescence Filter accessory has picture
7092185 670/10 nm Fluorescence Filter accessory has picture
7092186 670/20 nm Fluorescence Filter accessory has picture
7092187 680/10 nm Fluorescence Filter accessory has picture
7092188 680/20 nm Fluorescence Filter accessory has picture
7082229 680/30 nm Fluorescence Filter accessory has picture
7092189 690/10 nm Fluorescence Filter accessory has picture
7092190 700/20 nm Fluorescence Filter accessory has picture
7092191 710/20 nm Fluorescence Filter accessory has picture
7092192 730/40 nm Fluorescence Filter accessory has picture
7092193 760/20 nm Fluorescence Filter accessory has picture
7092194 760/34 nm Fluorescence Filter accessory has picture
7092195 781/20 nm Fluorescence Filter accessory has picture
7092196 785/20 nm Fluorescence Filter accessory has picture
7092197 807/25 nm Fluorescence Filter accessory has picture
7092198 818/40 nm Fluorescence Filter accessory has picture

Labware Holders

7302030 Cuvette Adapter accessory has picture
7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture

Mirrors

7132121 Half-size dichroic mirror, 50%, Ex 200-850 nm, Em 200-850 nm accessory has picture
7137400 Full-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7137435 Full-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7137455 Full-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7137510 Full-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7137525 Full-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7137545 Full-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7137550 Full-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7137555 Full-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7137570 Full-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7137595 Full-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7137660 Full-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture
7138320 Half-Size Dichroic Mirror, Ex. 260-305, Em. 335-750 accessory has picture
7138365 Half-Size Dichroic Mirror, Ex. 290-350, Em. 380-800 accessory has picture
7138400 Half-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7138435 Half-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7138455 Half-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7138510 Half-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7138525 Half-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7138545 Half-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7138550 Half-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7138555 Half-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7138570 Half-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7138595 Half-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7138660 Half-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture

Miscellaneous

1210007 Gas Controller for CO2 control. Includes CO2 flow meter & tubing set (p/n 1213032) accessory has picture
1210008 Gas Controller for CO2 and O2 control. Includes CO2 and N2 flow meters & tubing set (p/n 1213033 and p/n 1213032). accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

8040528 Synergy H1 Qualification Package

Reagent Injector Options

1320514 Angled tip injector head accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3TRIO Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7092092 Fluorescence Test Plate accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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