Synergy H1 Hybrid Multi-Mode Microplate Reader
Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader that can be turned into a high-performance patented Hybrid system with the addition of a filter-based optical module. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories.
The filter module is a completely independent add-on that includes its own light source, and a high performance dichroic-based wavelength selection system. With its very high optical efficiency, this module supports advanced detection modes such as Fluorescence Polarization, Time-Resolved Fluorescence & TR-FRET and filtered luminescence (e.g. BRET). A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).
To create the ideal physiological environment for live-cell assays, the new Gas Controller for Synergy H1 allows control and monitoring of CO2 and O2 levels within the Synergy H1. The Gas Controller, along with user-adjustable orbital shaking and advanced 4-ZoneTM temperature control make up the CellControlTM feature set available for the Synergy H1 readers.
Hybrid Technology™ protected under U.S. Patent 8,218,141.
HTRF® and the HTRF logo are registered trademarks of Cisbio.
- Patented Hybrid Technology™ combines flexible monochromator detection with high performance dichroic-based detection.
- Compatible with the Gas Controller for control and monitoring of CO2 and O2.
- Compatible with Take3™ Micro-Volume Plate: samples down to 2 µL volume can be measured. Especially useful when working with precious samples, for fast and accurate DNA/RNA quantification at 260 nm
- Quadruple grating monochromator for maximum flexibility and ease of use.
- Dichroic-based filter optics, for best performance and advanced detection technologies such as fluorescence polarization and time resolved fluorescence.
- Comes with Gen5 software: reader control, advanced data analysis and flexible Excel export in one software package.
| Model | Synergy H1 | |||||
| Part # | H1M | H1MD | H1F | H1FD | H1MF | H1MFD |
| Monochromator fluorescence | • | • | • | • | ||
| Monochromator absorbance | • | • | • | • | ||
| Full-light luminescence | • | • | • | • | • | • |
| Filter/dichroic fluorescence | • | • | • | • | ||
| Fluorescence polarization | • | • | • | • | ||
| Time resolved fluorescence | • * | • * | • | • | • | • |
| Filtered luminescence | • | • | • | • | ||
| Dual reagent dispenser | • | • | • | |||
| Temperature control to 45°C | • | • | • | • | • | • |
| Gen5 data analysis software | • | • | • | • | • | • |
| * Secondary mode | ||||||
| Gas Controller Ready Models** | H1MG | H1MDG | H1FG | H1FDG | H1FMG | H1MFDG |
| ** These models have the same features as the non- Gas Controller Ready models in the chart above. Gas Controller sold separately. | ||||||
| General | |
| Wavelength Selection | Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141) Quadruple Monochromators and Filters / Dichroics |
| Detection mode | Monochromator system: Fluorescence, Luminescence, UV-Visible Absorbance Filter system: Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence |
| Read method | End point, kinetic, spectral scanning, well-area scanning |
| Microplate types | 1- to 384-well plates Compatible with Take3 Multi-Volume Plate with 2 µL microspots |
| Temperature control | To 45°C, ±0.5 °C at 37°C |
| Shaking | Yes |
| Software | Gen5™ reader control included, advanced data analysis, Microsoft® Excel® export. Control through USB or serial port |
| Automation | Yes |
| CO2 and O2 Control | Gas Controller option available for Synergy H1 models |
| Absorbance | |
| Light source | Xenon Flash Lamp |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increment |
| Bandpass | 4 nm (230-285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | ±2 nm |
| Monochromator wavelength repeatability | ±0.2 nm |
| OD accuracy | <1% at 2.0 OD typical <3% at 3.0 OD typical |
| OD linearity | <1% from 0 to 3.0 OD typical |
| OD repeatability | <0.5% at 2.0 OD typical |
| Stray light | 0.03% at 230 nm typical |
| Reading speed | 96: 11 seconds 384: 22 seconds |
| Fluorescence Intensity | |
| Sensitivity | Monochromators: Top: fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) Bottom: fluorescein 5 pM typical (0.5 fmol/well 384-well plate) Filters/mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate) |
| Light source | Xenon Flash Lamp |
| Wavelength selection | Double grating monochromators (Top and Bottom) and, Deep blocking bandpass filters / dichroic mirrors (Top) |
| Wavelength range | Monos: 250 - 700 nm Filters: 200 - 700 nm (850 option) |
| Dynamic range | 5 decades |
| Detection system | Two PMT detectors: one for monochromator system, one for filter system |
| Reading speed | 96: 11 seconds 384: 22 seconds |
| Luminescence | |
| Sensitivity | Monochromator system: 20 amol ATP typical (flash) Filter system: 10 amol ATP typical (flash) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Fluorescence Polarization | |
| Sensitivity | 5 mP at 1 nM fluorescein typical |
| Wavelength range | 320 - 700 nm (850 nm option) |
| Time-Resolved Fluorescence | |
| Light source | Xenon Flash Lamp |
| Sensitivity | Europium 100 fM typical with filters (10 amol/well in 384-well plate) Europium 1200 fM typical with monos (120 amol/well in 384-well plate) |
| Wavelength range | Monos: 250 - 700 nm Filters: 200 - 700 nm (850 option) |
| Reagent Dispensers | |
| Dispense Precision | <2% at 50-200 µL |
| Dispense Accuracy | ±1 µL or 2% |
| Number | 2 syringe pumps |
| Plate Geometry | 1- to 384-well microplates |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dead volume | 1 mL, 100 µL with back flush |
| Physical Characteristics | |
| Power | 130 Watts max |
| Dimensions | 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm) |
| Weight | 50 lbs (22.5 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Standard (Included)
Gen5 Data Analysis Software
Optional
BioStack3 Microplate Stacker
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