Synergy Neo HTS Multi-Mode Microplate Reader
Synergy™ Neo is an HTS multi-mode microplate reader designed specifically for today's screening and core laboratories. Synergy Neo has all the features expected in an HTS screening instrument, including multiple parallel detectors for ultra-fast measurements and a dedicated filter-based system for live cell assays. With a load/unload plate transfer time of about 6 seconds per plate, Synergy Neo ’s optional integrated plate stacker is the fastest stacker on the market, allowing walk-away automation of short- or long-term assays. Synergy Neo also meets today's diverse assay requirements by incorporating features such as a powerful 100 mW laser-based excitation source for Alpha assays, a unique patented Hybrid optical design, advanced temperature control to 65°C, orbital and linear shaking. Ease of use and user confidence are key to the design of Synergy Neo ’s unique filter cubes that are barcode labeled for positive filter ID - limiting the possibility of errors and streamlining the workflow, while an available image-based barcode reader can scan both 1D and 2D barcode-labeled plates. The outstanding functionality of Synergy Neo is controlled by the powerful, yet easy to use, Gen5 2.0 Data Analysis Software. If you are involved in the HTS screening world, Synergy Neo was designed just for you.
Hybrid Technology™ protected under U.S. Patent 8,218,141.
HTRF® and the HTRF logo are registered trademarks of Cisbio.
- 4 PMT detectors including a dual PMT read head for ultra-fast ratiometric measurements.
- Fastest plate stacker on the market with a plate exchange time of 6 seconds
- Powerful 100 mW laser-based excitation for Alpha assays
- Patented Hybrid optical system: top and bottom filter-based optics for best speed and performance; top and bottom monochromator optics for ultimate wavelength flexibility.
- Advanced 4-Zone™ incubator with temperature control to 65°C to cover the broadest range of incubated assays
- Multi-directional barcode reader supports standard 1-D barcodes and 2-D barcodes
- Very fast reading speeds: 6 seconds for 96 well plates, 11 seconds for 384 well plates
NEOB
Synergy Neo HTS Multi-Mode Reader with top dual PMT & bottom filter optics, top & bottom monochromator optics, fluorescence intensity, fluorescence polarization, time resolved fluorescence, UV-Vis absorbance, luminescence.
NEOALPHAB
Synergy Neo HTS Multi-Mode Reader with top dual PMT & bottom filter optics, top & bottom monochromator optics, Alpha laser, fluorescence intensity, fluorescence polarization, time resolved fluorescence, UV-Vis absorbance, luminescence.
| General | |
| Wavelength Selection | Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141) Quadruple monochromators and barcode-labeled filter cubes |
| Detection mode | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
| Read method | End point, kinetic, spectral scanning, well area scanning |
| Microplate types | 1- to 1536-well plates Compatible with Take3™ Micro-Volume Plates with 2 µL microspots |
| Temperature control | 3° C above ambient to 65° C + 0.5° C at 37° C |
| Shaking | Linear, orbital, double orbital |
| Read height | Auto Z, 0.1 mm steps, top/bottom (filters), top (mono) |
| Kinetic speed | 96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds |
| With Stacker, minimum processing time per plate | 96 well: 20 seconds 384 well: 25 seconds 1536 well: 39 seconds |
| Barcode reader | Multi-directional, 1D and 2D camera-based scanner |
| Software | Gen5™ Reader Control and Data Analysis Included Control through USB or serial port |
| AlphaScreen/AlphaLISA | |
| Light source | 100 mW 680 nm laser |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
| Reading speed | 96 well: 30 seconds 384 well: 1 minute 50 seconds 1536 well: 7 minutes 20 seconds |
| Wavelength Selection | Filter cubes |
| Absorbance | |
| Light source | Xenon flash lamp |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increment |
| Bandpass | 2 nm (230-285 nm), 4 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Fluorescence Intensity | |
| Sensitivity | Filter cubes: Quadruple Monochromator: |
| Light source | Xenon flash lamp |
| Wavelength selection | Double grating monochromators (top/bottom) and, filter cubes (top/bottom) |
| Wavelength range | Monochromators: 250 - 850 nm Filters Cubes: 200 - 850 nm |
| Bandpass | Monochromators: 16 nm excitation / emission Filters: filter-dependant, from 5 nm to >100 nm |
| Detection system | Two matched PMTs top filter system Low Noise PMT bottom filter system Red shifted PMT top/bottom monochromator system |
| Luminescence | |
| Sensitivity | 5 amol ATP typical (384-well low volume plate) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | > 6 decades |
| Fluorescence Polarization | |
| Light source | Xenon flash lamp |
| Sensitivity | 1 mP at 1 nM fluorescein typical (384-well low volume plate) |
| Wavelength selection | Filter cubes (top / bottom) |
| Wavelength range | 320 - 850 nm |
| Time-Resolved Fluorescence | |
| Light source | Xenon flash |
| Sensitivity | Europium 40 fM typical (384-well low volume plate) |
| Wavelength selection | Filter cubes (top/bottom) Double grating monochromator (top/bottom) |
| Detection System | Two matched PMTs |
| Wavelength range | Filters: 200 - 850 nm Monochromators: 250 - 850 nm |
| Reagent Dispensers | |
| Dispense Precision | < 2% at 50-200 µL |
| Dispense Accuracy | + 1 µL or 2% |
| Number | 2 syringe pumps |
| Plate Geometry | 6- to 384-well microplates |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dead volume | 1.1 ml, 100 µL with back flush |
| Physical Characteristics | |
| Power | 250 Watts max. |
| Dimensions | 16.1 x 15.4 x 20.7 in. (41 x 39 x 52.5 cm) - H x W x D |
| Weight | 78 lbs (35 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Optional | ||
| BIOSTACKNEO | BioStack for Synergy Neo | |
| 1030008 | Barcode Reader Option | |
BioStack Integration Kits | ||
| 7310039 | BioStackNEO alignment kit | |
Labware Holders | ||
| 7330531 | Holder for 60-/72-/96-well Terasaki Plates  |
|
Product Qualification Packages | ||
| 1030542 | Synergy Neo Product Qualification Package | |
Reagent Injector Assembly | ||
| 8040003 | Dual Reagent Dispenser Module option |
|
Take3 Micro-Volume Plate | ||
| TAKE3 | Take3 Micro-Volume Plate |
|
| Take3Trio | Take3 Trio Micro-Volume Plate |
|
Test Plates & Reagents | ||
| 7092092 | Fluorescence Test Plate |
|
| 7260522E | Absorbance Test Plate |
|
| 8030015 | Luminescence Test Plate |
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