Synergy Neo Multi-Mode Reader

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BioTek’s fastest, high performance multi-mode plate reader

Dual PMT read head for ultra-fast ratiometric assays

100 mW Alpha laser: speed and performance

Ultra-fast plate stacker: 6 second plate exchange time

Select ScienceSynergy™ Neo is a hybrid multi-mode microplate reader designed specifically for today's screening and core laboratories. Synergy Neo has all the features expected in an HTS screening instrument, including multiple parallel detectors for ultra-fast measurements and a dedicated filter-based system for live cell assays. With a load/unload plate transfer time of about 6 seconds per plate, Synergy Neo's optional integrated plate stacker is the fastest stacker on the market, allowing walk-away automation of short- or long-term assays. Synergy Neo also meets today's diverse assay requirements by incorporating features such as a powerful 100 mW laser-based excitation source for Alpha assays, a unique patented Hybrid optical design, advanced temperature control to 65°C, orbital and linear shaking. Ease of use and user confidence are key to the design of Synergy Neo's unique filter cubes that are barcode labeled for positive filter ID - limiting the possibility of errors and streamlining the workflow, while an available image-based barcode reader can scan both 1D and 2D barcode-labeled plates. The outstanding functionality of Synergy Neo is controlled by the powerful, yet easy to use, Gen5 2.0 Data Analysis Software.

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.

HTRF® and the HTRF logo are registered trademarks of Cisbio.

  • 4 PMT detectors including a dual PMT read head for ultra-fast ratiometric measurements.
  • Fastest plate stacker on the market with a plate exchange time of 6 seconds
  • Powerful 100 mW laser-based excitation for Alpha assays
  • Patented Hybrid optical system: top and bottom filter-based optics for best speed and performance; top and bottom monochromator optics for ultimate wavelength flexibility.
Fiber-free top optics light path with dual PMT detectors
  • Advanced 4-Zone™ incubator with temperature control to 65°C to cover the broadest range of incubated assays
  • Multi-directional barcode reader supports standard 1-D barcodes and 2-D barcodes
  • Very fast reading speeds: 6 seconds for 96 well plates, 11 seconds for 384 well plates

Alpha technologies are registered trademarks of PerkinElmer, Inc.
Part #
Top monochromator optics
Top filter optics
Top dual PMT
Bottom monochromator optics
Bottom filter optics
Alpha laser
Fluorescence intensity,
fluorescence polarization,
time-resolved fluorescence,
UV-Vis absorbance, luminescence
BioStackNeo, BioStack4 and BioStack3 compatible


Wavelength selection Hybrid Technology™ (patented)
Quadruple Monochromators and Filter Cubes
Detection modes Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, Alpha, Luminescence, UV-Visible Absorbance
Read methods End point, kinetic, spectral scanning, well area scanning
Microplate types 1- to 1536-well plates
Other labware Take3™ Micro-Volume Plates
Temperature control 4-Zone™ incubation to 65 °C; variation +0.2 °C at 37 °C with Condensation Control™
Shaking Linear, orbital, double orbital
Software Gen5™ Data Analysis Software
Automation BioStack™ and 3rd party automation compatible
Read height Auto Z, 0.1 mm steps, top/bottom (filters), top (mono)
Kinetic speed 96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
With Stacker, minimum processing time per plate 96 well: 20 seconds
384 well: 25 seconds
1536 well: 39 seconds
Barcode reader Multi-directional, 1D and 2D camera-based
Light source Xenon flash
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandwidth 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Fluorescence Intensity
Light source Xenon flash
Sensitivity Monochromator:
Fluorescein 2 pM - top
Fluorescein 2.5 pM - bottom

Filter cubes:
Fluorescein 0.2 pM (384-well low volume plate) - top
Fluorescein 1 pM (1536-well plate) - top
Fluorescein 1 pM (384-well low volume plate) - bottom
Wavelength selection Double grating monochromators (top/bottom)
Filter cubes (top/bottom)
Wavelength range Monochromators: 250 - 850 nm
Filter Cubes: 230 - 850 nm
Bandwidth Monochromators: 16 nm excitation / emission
Filters: filter-dependant, from 5 nm to >100 nm
Detection system Two matched PMTs top filter system
Low Noise PMT bottom filter system
Red shifted PMT top/bottom monochromator system
Sensitivity 5 amol ATP typical (384-well low volume plate)
Wavelength range 300 - 700 nm
Dynamic range > 6 decades
Fluorescence Polarization
Light source Xenon flash
Sensitivity 1 mP standard deviation at 1 nM fluorescein
(384-well low volume plate)
1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
Wavelength selection Filter cubes (top / bottom)
Wavelength range 280 - 850 nm
Time-Resolved Fluorescence
Light source Xenon flash
Sensitivity Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate)
Wavelength selection Double grating monochromator (top/bottom)
Filter cubes (top/bottom)
Detection system Two matched PMTs
Wavelength range Monochromators: 250 - 850 nm
Filters: 200 - 850 nm
Read speed 96 well: 30 seconds
384 well: 1 minute 50 seconds
1536 well: 7 minutes 20 seconds
Light source 100 mW 680 nm laser
Wavelength selection Filter cubes
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 ml, 100 µL with back flush
Plate geometry 6- to 384-well plates, Petri dishes
Dispense precision < 2% at 50-200 µL
Dispense accuracy + 1 µL or 2%
Physical Characteristics
Power 250 Watts max.
Dimensions 15.4" W x 20.7" D x 16.1" H (39 x 52.5 x 41 cm)
Weight 78 lbs (35 kg)
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


BioStack Integration Kits

7310039 BioStackNEO alignment kit

Filter Cubes

1035001 Alpha Excitation
1035002 Alpha Emission 570/100 nm
1035003 Top single PMT fluorescence/ Luminescence
1035004 All Dual FP assays
1035005 Alpha 1536 Emission 570/100 nm
1035011 HTRF-Europium excitation 330 nm
1035012 HTRF Terbium / LanthaScreen Terbium excitation 340 nm
1035013 CFP-YFP FRET excitation 420 nm
1035014 FITC-TRITC, EGFP-Cy3, EGFP-YFP FRET excitation 485 nm
1035015 Cy3-Cy5 FRET excitation 540 nm
1035016 GeneBLAzer, Z-Lyte excitation 400 nm
1035017 LANCE excitation 360 nm
1035041 HTRF, LANCE red emission 620/665 nm
1035042 LanthaScreen emission 495/520 nm
1035043 CFP-YFP FRET emission 485/540
1035044 FITC-TRITC FRET emission 528/575 nm
1035045 Cy3-Cy5 FRET emission 590/680
1035046 EGFP-Cy3 FRET emission 516/590
1035047 EGFP-YFP FRET emission 516/540
1035048 GeneBLAzer, Z-Lyte emission 460/528
1035049 HTRF green emission 520/620
1035061 Dual FP Green 485/528
1035062 Dual FP red1 530/590
1035063 Dual FP red2 590/635
1035064 Dual FP far red 620/680
1035066 Dual FP UV 360/460
1035067 Dual Filter Cube UV 340/530
1035071 BRET1 Dual PMT
1035072 BRET2 Dual PMT
1035101 GFP excitation / emission – luminescence
1035102 GeneBLAzer
1035104 EGFP-Cy3 FRET
1035106 Cy3-Cy5 FRET
1035107 Blue (360/460) – Green (485/528) fluorescence
1035108 Green FP 485/528
1035109 BRET2 410/515
1035110 Far red FP 620/680
1035111 Green (485/528) – Red (530/590) fluorescence
1035112 Delfia Europium 360/620
1035113 Omnia (360/485) / Luminescence
1035114 Luminescence standard / 1536
1035115 Red (530/590) – Far Red (620/680)
1035116 Fura 2 (Ex 340, 380; Em 508)
1035117 BRET 1 cube, 460/40 and 540/25 nm
1035118 Deep UV FP 284/340 for use with Synergy Neo


BIOSTACKNEO BioStack for Synergy Neo
1030008 Barcode Reader Option

Product Qualification Packages

1030542 Synergy Neo Product Qualification Package

Reagent Injector Assembly

8040003S Dual Reagent Injector Module accessory has picture

Reagent Injector Options

1320514 Angled tip injector head accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3TRIO Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

7092092 Fluorescence Test Plate accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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