Synergy Neo2 Multi-Mode Reader

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BioTek’s fastest, Highest Performance multi-mode reader

BioTek’s fastest, Highest Performance multi-mode reader

Scientific quad monochromators with continuously variable bandwidth from 3 nm to 50 nm

Scientific quad monochromators with continuously variable bandwidth from 3 nm to 50 nm

Multiple PMTs, including dual top PMTs for ultra-fast ratiometric assays

Multiple PMTs, including dual top PMTs for ultra-fast ratiometric assays

Live cell assay optimization: CO<sub>2</sub>/O<sub>2</sub> control, incubation to 65&deg;C,  direct bottom illumination

Live cell assay optimization: CO2/O2 control, incubation to 65°C, direct bottom illumination

Patented Hybrid OpticsSynergy™ Neo2 Multi-Mode Microplate Reader is designed for speed and ultra high performance, incorporating BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance. Continuously variable bandwidth quadruple monochromators, sensitive high transmission filter-based optics and up to 4 PMTs provide ultra-fast measurements with excellent results. Advanced environment controls, including available CO2/O2 control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Barcode-labeled filter cubes help streamline workflows and limit errors, while the available BioStackNeo plate stacker is the fastest on the market - ideal for high throughput requirements. Powerful Gen5™ Data Analysis Software is included, and features designed for 21 CFR Part 11 compliance are available in Gen5 Secure.


BioStack and Neo2
The available BioStack Neo integrates with Synergy Neo2 for fast automated processing of up to 50 plates.
 

Synergy Neo2 is the recipient of the Best New Life Sciences Product of 2015.

 

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Hybrid Technology™ protected under U.S. Patent 8,218,141.
HTRF® and the HTRF logo are registered trademarks of Cisbio.

  • Patented Hybrid Technology™ with independent filter and monochromator-based optics
  • Scientific quad monochromators with continuously variable bandwidth for optimal sensitivity
  • High performance filter system
  • Ultra-fast plate processing speeds with multiple PMT detectors
  • Live cell options: Controlled environment and direct bottom detection
  • Driven by best-in-class Gen5™ software for data collection and analysis
  • 21 CFR Part 11 Compliance with available Gen5 Secure Software
Fiber-free top optics light path with dual PMT detectors
 
  •  Modular and upgradable

Synergy Neo2 - modular and upgradable

Ultra Fast Detection Configurations include dual top PMTs for fluorescence and luminescence.

 
Monochromator-based
Filter-based
Laser Alpha
 
UV-Vis absorbance
top/bottom FI
FI/FP/TRF
bottom FI/FP/TRF
luminescence
top
NEO2
 
 
 
NEO2B
 
 
NEO2M
 
 
NEO2MB
 
NEO2ALPHA
 
 
NEO2ALPHAB
 
NEO2MALPHA
 
 •
NEO2MALPHAB
 •
 •


Performance & Value Configurations include a single top PMT for fluorescence and luminescence.

 
Monochromator-based
Filter-based
Laser Alpha
 
UV-Vis absorbance
top/bottom FI
FI/FP/TRF
bottom FI/FP/TRF
luminescence
top
NEO2S
 
 
 
NEO2SB
 
 
NEO2SM
 
 
NEO2SMB
 
NEO2SALPHA
 
 
NEO2SALPHAB
 
NEO2SMALPHA
 
 •
NEO SMALPHAB

 •
 •

 

 

General
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types 6- to 1536-well plates
Other labware supported Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 65 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: + 0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2%at 1% O2
Models for CO2 /O2 or CO2 only are available
Barcode reader 1D and 2D camera-based scanner
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
Fluorescence Intensity
Light source Xenon flash
Detector Dual top PMTs
Single top PMT (option)
Low noise PMT (bottom filter system)
Red shifted PMT (top/bottom monochromator system)
Wavelength selection Quad monochromators (top/bottom)
Filters (top/bottom)
Wavelength range Monochromators: 250 - 850 nm
Filters (dual PMT): 200 - 850 nm
Monochromator bandwidth Variable, from 3 to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) - top
Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) - top
Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) - bottom

Quad monochromator:
Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) - top
Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) - bottom
Reading speed (kinetic) 96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
Luminescence
Wavelength range 300 - 700 nm
Dynamic range > 6 decades
Sensitivity 5 amol ATP typical (384-well low volume plate)
Fluorescence Polarization
Light source Xenon flash
Detector Dual PMT or single PMT (option)
Wavelength selection Filters
Wavelength range 280 - 850 nm
Sensitivity 1 mP standard deviation at 1 nM fluorescein
(384-well low volume plate)
1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
Time-Resolved Fluorescence
Light source Xenon flash
Detector Dual PMT or single PMT (option)
Wavelength selection Quad monochromators (top/bottom)
Filters (top/bottom)
Wavelength range Monos: 250 - 850 nm
Filters (dual PMT): 200 - 850 nm
Sensitivity Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate)
Alpha Detection
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Read speed 96 well: 30 seconds
384 well: 1 minute 50 seconds
1536 well: 7 minutes 20 seconds
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri dishes
Dead volume 1.1 ml, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy + 1 µL or 2%
Dispense precision < 2% at 50-200 µL
Physical Characteristics
Power 250 Watts max.
Dimensions 15.4" W x 20.7" D x 16.1" H
(39 x 52.5 x 41 cm)
Weight 78 lbs (35 kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Microplate Reader and Imager Software

Gen5 Microplate Reader and Imager Software

BioTek’s Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.
Note: Some instruments come with a specific edition of Gen5, please check the microplate reader specifications.


Optional

BioSpa 8 Automated Incubator

BioSpa 8 Automated Incubator

BioSpa 8 is an automated incubator linking BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Optional

BioSpa 8 Integration Kits

1410008 Alignment kit for Synergy Neo2 and Cytation 3/5

BioStack Integration Kits

7310055 BioStack integration kit for Synergy Neo2

Filter Cubes

1035001 Alpha (96-/384-well plates): EM 570/100
1035002 Alpha top cube for dual PMT system
1035003 Top single PMT fluorescence/luminescence
1035004 All Dual FP assays
1035005 Alpha (1536-well plates): EM 570/100
1035006 AlphaPlex lower cube
1035011 HTRF-Europium: EX 330/80 | DM 365
1035012 HTRF Terbium, LanthaScreen Terbium: EX 340/30 | DM 400
1035013 CFP-YFP FRET: EX 420/50 | DM 455
1035014 FITC-TRITC, EGFP-Cy3, EGFP-YFP FRET: EX 485/20 | DM 510
1035015 Cy3-Cy5 FRET: EX 540/25 | DM 550
1035016 GeneBLAzer, Z-Lyte: EX 400/30 | DM 435
1035017 LANCE: EX 360/40 | DM 400
1035041 HTRF, LANCE red: EM 620/10,665/8 | DM 650
1035042 LanthaScreen: EM 495/10, 520/25 | DM 510
1035043 CFP-YFP FRET: EM 485/20, 540/25 | DM 510
1035044 FITC-TRITC FRET: EM 528/20, 575/15 | DM 550
1035045 Cy3-Cy5 FRET: EM 590/35, 680/30 | DM 660
1035046 EGFP-Cy3 FRET: EM 516/20, 590/35 | DM 570
1035047 EGFP-YFP FRET: EM 516/20, 540/25 | DM 525
1035048 GeneBLAzer, Z-Lyte: EM 460/40, 528/20 | DM 510
1035049 HTRF green: EM 520/10, 620/10 | DM 550
1035061 Dual FP Green 485/528
1035062 Dual FP red1 530/590
1035063 Dual FP red2 590/635
1035064 Dual FP far red 620/680
1035066 Dual FP UV 360/460
1035067 Dual Filter Cube UV 340/530
1035071 BRET1 (480/530): DM 510
1035072 BRET2 (400/510): DM 470
1035073 Dual Alpha (AlphaPlex): EM 540/25, 615/16 | DM 570
1035074 NanoBRET: EM 450/50, 610LP | DM 550
1035101 GFP excitation / emission – luminescence
1035102 GeneBLAzer
1035103 FITC-TRITC FRET
1035104 EGFP-Cy3 FRET
1035105 EGFP-YFP FRET
1035106 Cy3-Cy5 FRET
1035107 Blue (360/460) – Green (485/528) fluorescence
1035108 Green FP 485/528
1035109 BRET2 410/515
1035110 Far red FP 620/680
1035111 Green (485/528) – Red (530/590) fluorescence
1035112 Delfia Europium 360/620
1035113 Omnia (360/485) / Luminescence
1035114 Luminescence standard / 1536
1035115 Red (530/590) – Far Red (620/680)
1035116 Fura 2 (Ex 340, 380; Em 508)
1035117 BRET 1 cube, 460/40 and 540/25 nm
1035118 Deep UV FP 284/340 for use with Synergy Neo
1035119 Single PMT HTRF Red
1035120 Single PMT FP 530/590 FP
1035121 Single PMT Lum 485/Lum 600
1035122 NanoBRET single PMT 450/50 nm and 610LP
1035255 Custom cube #255

Miscellaneous

1030008 Barcode Reader Option
1210012 CO2 - only gas controller accessory has picture
1210013 CO2 and O2 gas controller accessory has picture

Product Qualification Packages

1350526 Synergy Neo2 Product Qualification Package - IQ/OQ/PQ

Reagent Injector Assembly

8040030 Dual reagent injector module for use with Synergy Neo2 accessory has picture

Reagent Injector Options

1320514 Angled tip injector head accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3TRIO Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

7260522E Absorbance Test Plate accessory has picture
7260551 340 nm Absorbance Test Plate
8030015 Luminescence Test Plate accessory has picture

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