Synergy HTX Multi-Mode Reader

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Synergy HTX Multi-Mode Reader

Great flexibility at a low price!

1. Monochromator - based UV/Vis absorbance<br>2. Filter-based fluorescence<br>

1. Monochromator - based UV/Vis absorbance
2. Filter-based fluorescence

Excellent performance with AlphaScreen® and AlphaLISA®

Excellent performance with AlphaScreen® and AlphaLISA®

Dual Reagent Injectors are ideal for inject/read assays

Dual Reagent Injectors are ideal for inject/read assays

Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. Absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® measurements are all made using a unique dual-optics design that provides superior performance. The absorbance detection optics uses a xenon flash lamp and monochromator for filter-free wavelength selection from 200 to 999 nm in 1 nm increments. Fluorescence determinations are made using a tungsten halogen lamp with interference filters in conjunction with a PMT detector for maximum sensitivity. Synergy HTX also features BioTek’s unique 4-Zone™ incubation to 50 ºC, available dual reagent injector module, plus linear and orbital shaking to meet a wide variety of assay requirements. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 Data Analysis Software for data collection, analysis, exporting and reporting. For increased workflow automation and throughput, BioTek’s BioStack can be easily connected to Synergy HTX to automatically process up to 50 microplates at a time. For convenience, versatility and affordability, Synergy HTX is the ideal multi-mode microplate reader.

US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).
 

Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.
“Alpha” refers to products/technologies from PerkinElmer, Inc., that carry trademarks or registered trademarks.

 

Take3 Trio Compatible

  • Flexibility of monochromator based absorbance with high performance filter-based fluorescence/luminescence
  • Low volume (2 µL) nucleic acid and protein quantification with available Take3 Micro-Volume Plates
  • Cell friendly orbital shaking and advanced 4-Zone™ incubator design to 50 °C with Condensation Control™
  • Optional dual reagent injector module with angled tips, for inject/read applications, such as enzyme kinetics and Dual-Luciferase® Reporter
  • AlphaScreen®/AlphaLISA® assay capable
  • Modular and upgradable
  • Powerful Gen5 Software for reader control and all date reduction needs
  • Advanced shaking profiles including linear and orbital
  • Compatible with BioStack and 3rd party automation
  • Cold room (4 °C or 10 °C) compatible

Synergy HTX has a unique dual optics design that uses 1) a monochromator for UV-Vis absorbance and 2) high quality filters for fluorescence, providing versatility and excellent performance.

 


 
 Part #

S1L

S1A S1LA S1LF S1LFA

S1LFTA

 Absorbance    
 Fluorescence      
 Luminescence  
 Incubation
 Shaking
 Dual Reagent Injector*
 TRF (secondary mode)          
 Software Gen5™ included with all configurations
*Dual Reagent Injector module is optional, must be purchased separately

 

General
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Time-resolved fluorescence (secondary mode)
Alpha
Read methods End point, kinetic, spectral scanning and well area scanning
Microplate types 6- to 384-well plates
Other labware supported PCR plates, Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 50 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 - 999 nm, 1 nm increments
Monochromator bandwidth 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 14 seconds
384 wells: 26 seconds
Fluorescence Intensity
Light source Tungsten halogen
Xenon flash (option)
Detector PMT
Wavelength selection Filters
Wavelength range 300 - 700 nm (200 - 850 nm option)
Dynamic range >6 decades
Sensitivity Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate)
Reading speed (kinetic) 96 wells: 31 seconds
384 wells: 80 seconds
Luminescence
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash) - Lum and Abs / Lum configurations
30 amol ATP (flash) - Multi-mode configurations
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Monochromator
Alpha Detection
Light source Tungsten halogen
Detector PMT
Wavelength selection Filters
Sensitivity 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
Read speed 2 minute (96-well plate)
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates
Dead volume 1.1 mL with back flush
Dispense volume 5-1000 µL in 1 nm increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Connectivity 1 USB, 1 RS232 for external PC control
Power 100-240 VAC, 50/60 Hz
Dimensions 16"W x 15"D x 10"H
(40.6 x 38 x 25.4 cm)
Weight 40 lbs (18 kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Microplate Reader and Imager Software

Gen5 Microplate Reader and Imager Software

BioTek’s Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.
Note: Some instruments come with a specific edition of Gen5, please check the microplate reader specifications.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Standard (Included)

Cables

75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310046 BioStack Integration Kit for Synergy HTX accessory has picture

Bottles & Tubing

7112186 3 feet (1 meter) tubing for Synergy dispenser box

Cables

75053 Serial Cable, Null Modem (9-pin female to 25-pin female) accessory has picture

Filter Accessories

7080541 Empty Filter Wheel for Synergys and FLx800 accessory has picture
7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filters

7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092205 325/50 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7092159 485/10 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7092211 525/40NM Pierce Renilla-Firefly Dual Luciferase Green Renilla
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture
7082210 545/40 nm Fluorescence Filter accessory has picture
7092170 550/10 nm Fluorescence Filter accessory has picture
7092171 556/10 nm Fluorescence Filter accessory has picture
7092172 560/10 nm Fluorescence Filter accessory has picture
7082215 560/15 nm Fluorescence Filter accessory has picture
7082211 560/20 nm Fluorescence Filter accessory has picture
7082212 560/40 nm Fluorescence Filter accessory has picture
7082271 565/10 nm Fluorescence Filter
7092173 570/10 nm Fluorescence Filter accessory has picture
7082264 570/100 nm Fluorescence Filter accessory has picture
7092174 575/10 nm Fluorescence Filter accessory has picture
7082245 575/15 nm Fluorescence Filter accessory has picture
7092175 580/10 nm Fluorescence Filter accessory has picture
7082244 580/50 nm Fluorescence Filter accessory has picture
7092176 585/10 nm Fluorescence Filter accessory has picture
7092177 590/10 nm Fluorescence Filter accessory has picture
7082225 590/20 nm Fluorescence Filter accessory has picture
7082224 590/35 nm Fluorescence Filter accessory has picture
7092178 596/15 nm Fluorescence Filter accessory has picture
7082252 600/40 nm Fluorescence Filter accessory has picture
7092179 610/10 nm Fluorescence Filter accessory has picture
7092207 615/10NM Lanthascreen Europium donor emission
7082251 620/15 nm Fluorescence Filter accessory has picture
7082213 620/40 nm Fluorescence Filter accessory has picture
7092180 625/10 nm Fluorescence Filter accessory has picture
7092181 635/20 nm Fluorescence Filter accessory has picture
7082214 635/32 nm Fluorescence Filter accessory has picture
7092182 645/10 nm Fluorescence Filter accessory has picture
7082257 645/15 nm Fluorescence Filter accessory has picture
7082226 645/40 nm Fluorescence Filter accessory has picture
7092183 660/10 nm Fluorescence Filter accessory has picture
7092208 665/10NM Lanthascreen Europium acceptor emission
7092184 665/34 nm Fluorescence Filter accessory has picture
7092185 670/10 nm Fluorescence Filter accessory has picture
7092186 670/20 nm Fluorescence Filter accessory has picture
7092187 680/10 nm Fluorescence Filter accessory has picture
7092188 680/20 nm Fluorescence Filter accessory has picture
7082229 680/30 nm Fluorescence Filter accessory has picture
7092189 690/10 nm Fluorescence Filter accessory has picture
7092190 700/20 nm Fluorescence Filter accessory has picture
7092191 710/20 nm Fluorescence Filter accessory has picture
7092192 730/40 nm Fluorescence Filter accessory has picture
7092193 760/20 nm Fluorescence Filter accessory has picture
7092194 760/34 nm Fluorescence Filter accessory has picture
7092196 785/20 nm Fluorescence Filter accessory has picture
7092197 807/25 nm Fluorescence Filter accessory has picture
7092198 818/40 nm Fluorescence Filter accessory has picture
7092209 LP610NM Chromaglo red emitting luciferase
7092210 LP640NM Pierce Renilla-Firefly Dual Luciferase Red Firefly

Labware Holders

7302030 Cuvette Adapter accessory has picture

Miscellaneous

1340001 Dual Reagent Injector Module
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

1340508 Synergy HTX Qualification Package - IQ/OQ/PQ

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
Gen5 Gen5 Data Analysis Software accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3TRIO Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7092092 Fluorescence Test Plate accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7260522E Absorbance Test Plate accessory has picture
7260551 340 nm Absorbance Test Plate
8030015 Luminescence Test Plate accessory has picture

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