BioTek: Microplate Instrumentation & Software Solutions
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Synergy 2 Alpha Microplate Reader

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Upgradable to Multi-Mode Functionality

Synergy 2 Alpha is a microplate reader dedicated to the life science research applications of Alpha-based assay technologies (e.g. AlphaScreen®, AlphaLISA®, SureFire®). Its unique optical design makes it the most cost-effective Alpha reader on the market. In addition, Synergy 2 Alpha can be upgraded with other detection modes, such as UV-visible absorbance, fluorescence intensity, luminescence and more to accommodate a broad range of microplate applications. Whether you are looking for a dedicated Alpha reader, or a high-performance multimode reader, Synergy 2 Alpha has been designed to meet your needs.

Proven Performance on a Wide Range of Alpha Assays


Human Insulin Concentration Curve. AlphaLISA signal plotted against insulin
concentration. A logistic 4-parameter best fi t applied to the data using Gen5™
Data Analysis Software. Limit of detection: 2.8 µIU/mL of insulin.


AlphaScreen® cAMP Assay Data. cAMP concentrations from 5.0x10-6 M to
5.0x10-11 M, 25 µL/well in 384-well plate. Z' = 0.80. Signal/ Background = 22.5


Phosphorylation of p-p38MAPK protein stimulated with EGF, Sorbitol, Anisomycin, and TNF-a measured at 0, 5', 10', 15', and 30'.


AlphaScreen®, AlphaLISA®, SureFire® are registered trademark of Perkin Elmer.

  • Low cost / high performance Alpha optics combines Tungsten Halogen light source with ultra low noise PMT detector.
  • Comes with pre-programmed protocol files, sample data, and detailed application notes
  • Proven sensitivity: limit of detection <100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate, on par with dedicated laser-based detection systems
  • Ideal for life science research applications of Alpha-based assays
  • Upgradeable to Multi-Mode functionality: available detection modules include UV-Visible absorbance, fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence and dual reagent dispenser.

AlphaScreen®, AlphaLISA®, SureFire® are registered trademark of Perkin Elmer.

Detection mode AlphaScreen®, AlphaLISA®, SureFire®
Read method End-point, kinetic, well-area scanning
Microplate types 1- to 384-well plates
Maximum Plate Height 1.20" (30.5 mm)
Temperature control 4°C above ambient to 65°C ± 0.5°C at 37°C
Shaking Yes
Software Gen5™ included
Control through USB or serial port
Automation Yes
Light source Tungsten Halogen Lamp
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µ­L/well in 384-well plate
Dynamic range >6 decades
Reading speed 2 minutes typical for 96-well plate
Detection system Ultra Low Noise PMT
Physical Characteristics
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 55 lbs (25 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Standard (Included)


75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

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