Synergy 2 Alpha Microplate Reader
Synergy 2 Alpha is a microplate reader dedicated to the life science research applications of Alpha-based assay technologies (e.g. AlphaScreen®, AlphaLISA®, SureFire®). Its unique optical design makes it the most cost-effective Alpha reader on the market. In addition, Synergy 2 Alpha can be upgraded with other detection modes, such as UV-visible absorbance, fluorescence intensity, luminescence and more to accommodate a broad range of microplate applications. Whether you are looking for a dedicated Alpha reader, or a high-performance multimode reader, Synergy 2 Alpha has been designed to meet your needs.
Proven Performance on a Wide Range of Alpha Assays
AlphaLISA®
Human Insulin Concentration Curve. AlphaLISA signal plotted against insulin
concentration. A logistic 4-parameter best fi t applied to the data using Gen5™
Data Analysis Software. Limit of detection: 2.8 µIU/mL of insulin.
AlphaScreen®
AlphaScreen® cAMP Assay Data. cAMP concentrations from 5.0x10-6 M to
5.0x10-11 M, 25 µL/well in 384-well plate. Z' = 0.80. Signal/ Background = 22.5.
SureFire®
Phosphorylation of p-p38MAPK protein stimulated with EGF, Sorbitol, Anisomycin, and TNF-a measured at 0, 5', 10', 15', and 30'.
AlphaScreen®, AlphaLISA®, SureFire® are registered trademark of Perkin Elmer.
- Low cost / high performance Alpha optics combines Tungsten Halogen light source with ultra low noise PMT detector.
- Comes with pre-programmed protocol files, sample data, and detailed application notes
- Proven sensitivity: limit of detection <100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate, on par with dedicated laser-based detection systems
- Ideal for life science research applications of Alpha-based assays
- Upgradeable to Multi-Mode functionality: available detection modules include UV-Visible absorbance, fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence and dual reagent dispenser.
AlphaScreen®, AlphaLISA®, SureFire® are registered trademark of Perkin Elmer.
| General | |
| Detection mode | AlphaScreen®, AlphaLISA®, SureFire® |
| Read method | End-point, kinetic, well-area scanning |
| Microplate types | 1- to 384-well plates |
| Maximum Plate Height | 1.20" (30.5 mm) |
| Temperature control | 4°C above ambient to 65°C ± 0.5°C at 37°C |
| Shaking | Yes |
| Software | Gen5™ included Control through USB or serial port |
| Automation | Yes |
| AlphaScreen/AlphaLISA | |
| Light source | Tungsten Halogen Lamp |
| Sensitivity | 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate |
| Dynamic range | >6 decades |
| Reading speed | 2 minutes typical for 96-well plate |
| Detection system | Ultra Low Noise PMT |
| Physical Characteristics | |
| Dimensions | 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) |
| Weight | 55 lbs (25 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Standard (Included)
Gen5 Data Analysis Software
Optional
BioStack3 Microplate Stacker
Standard (Included) | ||
Cables | ||
| 75034 | Serial Cable, Modem (9-pin female to 9-pin male)  |
|
| 75108 | USB Cable (male A to male B) |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
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