Synergy 4 Hybrid Multi-Mode Microplate Reader
Synergy 4 with Hybrid Technology is the most advanced multi-detection microplate reader, designed to address the widest range of microplate-based applications. Its optical design combines a fluorescence filter-based detection system and a monochromator-based detection system in one compact unit. The filter-based system can be used when high sensitivity or fast read speeds are a requirement. The monochromator system is available when spectral scanning is needed.
Synergy 4 is the ideal instrument for core laboratories, assay development laboratories, or screening laboratories where assay requirements vary widely, and new molecules or processes are tested on a regular basis. With its hybrid optical design and three light sources, Synergy 4 offers users optimal assay performance over the widest range of applications.
This modular reader can be ordered fully loaded with all measurement modes or customized to fit budgetary requirements with the option to add other detection technologies at a later date. Available options include Filter-based and Monochromator-based Fluorescence Intensity (Hybrid Technology), Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen, UV-visible Absorbance, Flash and Glow Luminescence and Dual Reagent Injector. Features such as a precise temperature control system, plate shaking and advanced reader control and data analysis software are standard with all configurations.
Synergy 4 is certified for HTRF®, DLR™ and LanthaScreen™, and is compatible with AlphaScreen assays.
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The choice is yours.
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Filter-
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Monochromator-
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Hybrid
Technology |
| Spectral Scanning |
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| Flexible wavelength selection |
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| Convenience |
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| High performance Fluorescence Polarization |
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| High performance TRF / TR-FRET |
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| High performance across spectrum |
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| Ratiometric ion channel assays |
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| Filtered luminescence (e.g. BRET) |
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| AlphaScreen®/AlphaLisa® |
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US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).
HTRF® and the HTRF logo are registered trademarks of Cisbio.
Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.
AlphaScreen® is a registered trademark of Perkin Elmer.
Transcreener® is a registered trademark of BellBrook Labs.
The LanthaScreen™ and GeneBLAzer® technologies from Invitrogen have been validated on BioTek instruments.
- Hybrid Technology: Combined filter-based system with monochromator-based optics
Synergy 4’s hybrid optical design combines the sensitivity and speed of a filter-based system with the flexibility and convenience of a monochromator-based system.
- Compatible with Take3 Multi-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
- Modular architecture: Read modes are available as individual modules
- Detection modes and applications include: Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence, UV-Visible absorbance, FRET, TR-FRET, BRET, well area scanning and spectral scanning
- Quadruple grating system: Two double-grating monochromators
The Synergy 4 monochromator system uses two double-grating monochromators. Highest stray light rejection, continuous wavelength selection, spectral scanning: this system combines high performance with convenience and flexibility.
- Deep blocking filter and dichroic mirrors: provide the best possible performance in fluorescence, time-resolved fluorescence and fluorescence polarization
- Wide Range of Applications:
- Screening assays (e.g. fluorescence polarization, TR-FRET, luminescence), AlphaScreen®
- Spectral scanning
- Binding assays
- Ion channel assays
- Quantitative assays (DNA, protein)
- Kinetic assays
- Gene expression assays (GFP, Luciferase)
- ELISA assays
- Cell proliferation, Cytotoxicity
- Certified by reagent manufacturers: certified by Promega for the Dual Luciferase Reporter (DLR™) gene assay; high performance TRF detection certified by Cisbio for their HTRF® TR-FRET assay platform; LanthaScreen Certified+ from Invitrogen.
The Synergy 4 filter/mirror system delivers high levels of energy to the sample and provides high signal-to-noise ratios. Faster read speed, more sensitivity, more precise control over optical parameters: this system delivers ultimate performance.
Synergy 4 is a modular instrument. Read modes and options are available as individual modules at the time of purchase, allowing you to make the best use of your budget. In addition, existing units can be upgraded later when your research requirements expand.
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Synergy™ 4
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| Part # | Absorbance | LUM | FI (Filters & Monos) | FP | TRF | Injectors | Software |
| S4MFT | • | • | Gen5™ included | ||||
| S4MFTD | • | • | • | ||||
| S4MFTA | • | • | • | ||||
| S4MFTAD | • | • | • | • | |||
| S4MLFT | • | • | • | ||||
| S4MLFTD | • | • | • | • | |||
| S4MLFTA | • | • | • | • | |||
| S4MLFTAD | • | • | • | • | • | ||
| S4MLFPTA | • | • | • | • | • | ||
| S4MLFPTAD | • | • | • | • | • | • | |
| S4MFPT | • | • | • | ||||
| S4MFPTD | • | • | • | • | |||
| S4MLFPT | • | • | • | • | |||
| S4MLFPTD | • | • | • | • | • | ||
| S4MFPTA | • | • | • | • | |||
| S4MFPTAD | • | • | • | • | • | ||
| General | |
| Detection method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/AlphaLisa, Luminescence, UV-Visible Absorbance |
| Read method | End-point, kinetic, spectral scanning, well-area scanning |
| Microplate types | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates (luminescence 1-384) PCR plates Compatible with Take3 Multi-Volume Plate with 2 µL microspots |
| Temperature control | 4°C above ambient to 65°C +/- 0.5°C at 37°C |
| Shaking | Yes |
| Software | Gen5™ Included Control through USB or serial port |
| Automation | Yes |
| Absorbance | |
| Light source | SQ Xenon Flash |
| Wavelength selection | Monochromator |
| Wavelength range | 200 - 999 nm, 1 nm increment |
| Bandpass | 2.4 nm |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | +/- 2 nm |
| Monochromator wavelength repeatability | +/- 0.2 nm |
| OD accuracy | < 1% at 2.0 OD typical < 3% at 3.0 OD typical |
| OD linearity | < 1% from 0 to 3.0 OD typical |
| OD repeatability | < 0.5% at 2.0 OD typical |
| Stray light | 0.03% at 230 nm typical |
| Reading speed | 96: 11 seconds 384: 22 seconds 1536: 42 seconds |
| Fluorescence Intensity | |
| Sensitivity | Monochromators: fluorescein 5 pM typical (0.5 fmol/well 384-well plate) Filters/mirrors: fluorescein 1 pM typical (0.1 fmol/well 384-well plate) - Top fluorescein 5 pM typical (0.5 fmol/well 384-well plate) - Bottom |
| Light source | Tungsten Halogen High Energy DPR Xenon Flash |
| Wavelength selection | Double grating monochromators (Top) and, Deep blocking bandpass filters / dichroic mirrors (Top/Bottom) |
| Wavelength range | Monochromators: 250 - 800 nm Filters: 200 - 700 nm (900 nm option) |
| Dynamic range | > 6 decades |
| Bandpass | 12/12 (mono) Variable (filters) |
| Detection system | PMT |
| Reading speed | 96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 43 seconds |
| Luminescence | |
| Sensitivity | 10 amol ATP typical (flash) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | > 6 decades |
| Detection system | Low noise PMT |
| Fluorescence Polarization | |
| Light source | Tungsten Halogen High Energy DPR Xenon Flash (optional) |
| Sensitivity | 3 mP at 1 nM fluorescein typical |
| Wavelength selection | Deep blocking bandpass filters / dichroic mirrors (Top) |
| Wavelength range | 400 - 700 nm |
| Time-Resolved Fluorescence | |
| Light source | High Energy DPR Xenon Flash |
| Sensitivity | Europium 60 fM typical with filters (6 amol/well in 384-well plate) |
| Wavelength selection | Double grating monochromator (Top) and, Deep blocking bandpass filters / dichroic mirrors (Top / Bottom) |
| Wavelength range | Filters: 200 - 700 nm (900 nm option) Monochromators: 250 - 800 nm |
| Reagent Dispensers | |
| Number | 2 syringe pumps |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dead volume | 1.1 mL, 100 µL with backflush |
| Physical Characteristics | |
| Power | 250 Watts max. |
| Dimensions | 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) |
| Weight | 78 lbs (35 kg) |
| Regulatory | |
| Regulatory | For in vitro diagnostic use. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek. |
Specifications are subject to change.
Standard (Included)
Gen5 Data Analysis Software
Optional
BioStack Microplate Stacker
BioStack Twister II Microplate Handler
Standard (Included) | ||
Cables | ||
| 75034 | Serial Cable, Modem (9-pin female to 9-pin male)  |
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| 75108 | USB Cable (male A to male B) |
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Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
