Synergy 2 Multi-Mode Microplate Reader

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BioTek’s best price/performance ratio

The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. The dedicated absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. And, by utilizing dedicated optical elements for each individual detection technique, Synergy 2 offers uncompromised performance and the lowest limits of detection.

Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance. Its three broad-spectrum light sources have been chosen for optimal illumination and excitation in all applications.

The Synergy 2 is controlled by BioTek's Gen5™ Reader Control and Data Analysis Software, is certified for HTRF®, DLR™ and LanthaScreen™, and is compatible with AlphaScreen assays.

US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

HTRF® and the HTRF logo are registered trademarks of Cisbio.
Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.
AlphaScreen® is a registered trademark of Perkin Elmer.

Transcreener® is a registered trademark of BellBrook Labs.
The LanthaScreen™ and GeneBLAzer® technologies from Invitrogen have been validated on BioTek instruments.

 Take3 Trio Compatible

  • Measurement Modes: Fluorescense Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Alpha, Glow and Flash Luminescence, UV-Vis Absorbance, FRET, TR-FRET, BRET, Area Scanning, Spectral Scanning
  • Unique Optical System: a narrow band monochromator, optimized filter/dichroic mirror pairing, and three broad spectrum light sources achieve superior performance in all read modes
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • High Sensitivity: dedicated optical elements optimized for each specific modality result in the lowest limits of detection available in one compact instrument
  • Modular Design: purchase only what you need and easily upgrade later as experimental requirements expand
  • High Throughput: optimized for screening assays including 1536-well read mode
    The Synergy 2 offers small footprint
    The Synergy 2 offers a small footprint, a robotic-friendly carrier for easy integration, and reads a 384-well plate in only 22 seconds.
  • Wide Range of Applications: with built-in temperature control, shaking, and optional dual reagent injector, virtually any microplate-based application is possible
    • Screening assays (e.g. fluorescence polarization, TR-FRET, luminescence)
    • Binding assays
    • Ion channel assays
    • Quantitative assays (DNA, protein)
    • Kinetic assays
    • Gene expression assays (GFP, Luciferase)
    • ELISA assays
    • Cell proliferation, Cytotoxicity
  • High Performance Screening Assays: The Synergy 2 is used in screening laboratories with all the major assay technologies such as:
    • Cisbio International's HTRF® TR-FRET assays
    • Invitrogen Corporation's LanthaScreen™, PolarScreen™, Omnia™, Z'-Lyte®, GeneBLAzer®, VSP ion channel assays
    • AlphaScreen® and LANCE™ assays from Perkin Elmer, Inc.
    • Promega Corporation’s Glo® and flash luminescent assays
    Dual reagent injector
    Dual reagent injector for ion channel assays, flash luminescence and fast enzyme kinetics.
  • New Gen5™ Software: Synergy 2 is driven by the most powerful and efficient microplate control, data collection and analysis software available today.
    Gen5 Reader Control and Data Analysis Software
    Gen5™ Reader Control and Data Analysis Software

Synergy 2 is a modular instrument. Read modes and options are available as individual configurations at the time of purchase, allowing you to make the best use of your budget. In addition, existing units can be upgraded later when your research requirements expand.

Synergy™ 2 Custom Configurations
Contact BioTek for part #

 Module

 Description

 S  Synergy 2 base model.  Includes temperature control to 65ºC, Shaking, and Gen5™.
 L  Luminescence
 F  Fluorescence Intensity top and bottom, Alpha detection.  Includes Tungsten-Halogen light source.
 P  Fluorescence Polarization.  Requires F module.
 T  Time-Resolved Fluorescence.  Requires F module.  Includes high energy DPR Xenon Flash Lamp.
 A  UV-Visible Absorbance (monochromator-based).  Includes SQ Xenon Flash Lamp.
 D  Dual Reagent Dispenser

Red shifted PMT (900nm) option available instead of standard low noise PMT (700 nm).
3 mm diameter bottom fiber options available instead of standard 5 mm bottom fiber.

 

General
Detection mode Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa®, Luminescence, UV-Visible Absorbance
Read methods End point, kinetic, spectral scanning, well-area scanning
Microplate types 6- to 1536-well plates (luminescence 1-384)
PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4-Zone™ incubation to 65 °C
Variation+0.2 °C at 37 °C
Shaking linear
Software Gen5™ included
Control through USB or serial port
Automation Yes
AlphaScreen/AlphaLISA
Light source Tungsten Halogen Lamp
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate
Dynamic range >6 decades
Reading speed 2 minutes typical for 96-well plate
Detection system Ultra Low Noise PMT
Absorbance
Light source SQ Xenon Flash
Wavelength selection Monochromator
Wavelength range 200 - 999 nm, 1 nm increment
Bandpass 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2 OD typical
<3% at 3.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Fluorescence Intensity
Sensitivity Fluorescein 1 pM typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) - Top
Fluorescein 5 pM typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) - Bottom
Light source Tungsten Halogen
High Energy DPR Xenon Flash (optional)
Wavelength selection Deep blocking bandpass filters/dichroic mirrors
Wavelength range 300 - 700 nm with tungsten lamp (850 nm option)
200 - 700 nm with xenon lamp (850 nm option)
Dynamic range >6 decades
Bandwidth Filter dependent
Detection system PMT
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Luminescence
Sensitivity 10 amol ATP typical (flash)
100 amol ATP (glow) typical
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Detection system Low noise PMT
Fluorescence Polarization
Light source Tungsten Halogen
High Energy DPR Xenon Flash (optional)
Sensitivity 3 mP at 1 nM fluorescein typical
Wavelength selection Deep blocking filters/mirrors
Wavelength range 400 - 700 nm (320 - 850 nm option)
Time-Resolved Fluorescence
Light source High Energy DPR Xenon Flash
Sensitivity Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate)
Wavelength selection Deep blocking filters/mirrors
Wavelength range 200 - 700 nm (850 nm option)
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with backflush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm)
Weight 60 lbs (27 kg)
Regulatory
Regulatory CE and TUV marked, RoHS Compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Standard (Included)

Cables

75034 Serial Cable, Modem (9-pin female to 9-pin male) accessory has picture
75108 USB Cable (male A to male B) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310047 BioStack Integration Kit for Synergy 2, Synergy Mx, Synergy 4 and Synergy H4 accessory has picture

Bottles & Tubing

7112186 3 feet (1 meter) tubing for Synergy dispenser box

Filter Accessories

7080541 Empty Filter Wheel for Synergys and FLx800 accessory has picture
7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filters

7092202 274/5 nm Fluorescence Filter accessory has picture
7092146 276/10 nm Fluorescence Filter accessory has picture
7092145 276/5 nm Fluorescence Filter accessory has picture
7092148 280/10 nm Fluorescence Filter accessory has picture
7092147 280/5 nm Fluorescence Filter accessory has picture
7082259 284/10 nm Fluorescence Filter accessory has picture
7092149 284/5 nm Fluorescence Filter accessory has picture
7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7092151 355/20 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7092159 485/10 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture
7082210 545/40 nm Fluorescence Filter accessory has picture
7092170 550/10 nm Fluorescence Filter accessory has picture
7092171 556/10 nm Fluorescence Filter accessory has picture
7092172 560/10 nm Fluorescence Filter accessory has picture
7082215 560/15 nm Fluorescence Filter accessory has picture
7082211 560/20 nm Fluorescence Filter accessory has picture
7082212 560/40 nm Fluorescence Filter accessory has picture
7082271 565/10 nm Fluorescence Filter
7092173 570/10 nm Fluorescence Filter accessory has picture
7082264 570/100 nm Fluorescence Filter accessory has picture
7092174 575/10 nm Fluorescence Filter accessory has picture
7082245 575/15 nm Fluorescence Filter accessory has picture
7092175 580/10 nm Fluorescence Filter accessory has picture
7082244 580/50 nm Fluorescence Filter accessory has picture
7092176 585/10 nm Fluorescence Filter accessory has picture
7092177 590/10 nm Fluorescence Filter accessory has picture
7082225 590/20 nm Fluorescence Filter accessory has picture
7082224 590/35 nm Fluorescence Filter accessory has picture
7092178 596/15 nm Fluorescence Filter accessory has picture
7082252 600/40 nm Fluorescence Filter accessory has picture
7092179 610/10 nm Fluorescence Filter accessory has picture
7082265 620/10 nm Fluorescence Filter accessory has picture
7082251 620/15 nm Fluorescence Filter accessory has picture
7082213 620/40 nm Fluorescence Filter accessory has picture
7092180 625/10 nm Fluorescence Filter accessory has picture
7092181 635/20 nm Fluorescence Filter accessory has picture
7082214 635/32 nm Fluorescence Filter accessory has picture
7092182 645/10 nm Fluorescence Filter accessory has picture
7082257 645/15 nm Fluorescence Filter accessory has picture
7082226 645/40 nm Fluorescence Filter accessory has picture
7092183 660/10 nm Fluorescence Filter accessory has picture
7092184 665/34 nm Fluorescence Filter accessory has picture
7082266 665/8 nm Fluorescence Filter accessory has picture
7092185 670/10 nm Fluorescence Filter accessory has picture
7092186 670/20 nm Fluorescence Filter accessory has picture
7092187 680/10 nm Fluorescence Filter accessory has picture
7092188 680/20 nm Fluorescence Filter accessory has picture
7082229 680/30 nm Fluorescence Filter accessory has picture
7092189 690/10 nm Fluorescence Filter accessory has picture
7092191 710/20 nm Fluorescence Filter accessory has picture
7092192 730/40 nm Fluorescence Filter accessory has picture
7092193 760/20 nm Fluorescence Filter accessory has picture
7092194 760/34 nm Fluorescence Filter accessory has picture
7092195 781/20 nm Fluorescence Filter accessory has picture
7092196 785/20 nm Fluorescence Filter accessory has picture
7092197 807/25 nm Fluorescence Filter accessory has picture
7092198 818/40 nm Fluorescence Filter accessory has picture

Labware Holders

7302030 Cuvette Adapter accessory has picture
7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture

Mirrors

7132121 Half-size dichroic mirror, 50%, Ex 200-850 nm, Em 200-850 nm accessory has picture
7137400 Full-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7137435 Full-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7137455 Full-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7137510 Full-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7137525 Full-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7137545 Full-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7137550 Full-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7137555 Full-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7137570 Full-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7137595 Full-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7137660 Full-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture
7138320 Half-Size Dichroic Mirror, Ex. 260-305, Em. 335-750 accessory has picture
7138365 Half-Size Dichroic Mirror, Ex. 290-350, Em. 380-800 accessory has picture
7138400 Half-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7138435 Half-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7138455 Half-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7138510 Half-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7138525 Half-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7138545 Half-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7138550 Half-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7138555 Half-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7138570 Half-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7138595 Half-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7138660 Half-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture
7139635 Half-Size Dichroic Mirror, Ex. 640-780, Em. 400-630 accessory has picture
8030515S Empty Mirror Holder accessory has picture
8030516S FP Compatible Empty Mirror Holder accessory has picture

Miscellaneous

7132166 Optics Accessory Case accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

7130566 Synergy 2 Qualification Package

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture

Test Plates & Reagents

98155 Sodium Fluorescein Powder (1 mg vial) accessory has picture
7092092 Fluorescence Test Plate accessory has picture
7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7160006 Glowell Adapter for Synergy Luminescence Tests accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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