PowerWave Microplate Spectrophotometer
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BioTek’s PowerWave microplate spectrophotometers are designed for use in a myriad of applications, from direct quantitation of nucleic acids using automated pathlength correction to full-scale high throughput operations that demand speed, accuracy and reliability. With rugged hardware, proven optical performance and a very small footprint, PowerWave readers are uniquely designed to provide the most application flexibility while saving space both on the benchtop and in automated systems. Gen5 Data Analysis software comes with PowerWave readers and provides unsurpassed power and versatility.
Use PowerWave plate readers for applications requiring low UV measurements, such as direct DNA quantitation and purity testing, RNA quantitation, as well as visible range measurements for basic ELISA’s, enzyme kinetics, colorimetric assays, among many other applications.
US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).
- No Interference Filters Required
Monochromator optical design provides continuous wavelength selection from 200 nm to 999 nm (or 340 nm to 999 nm, depending on model). Measuring wavelengths can be selected in 1 nm increments to meet all assay requirements – it’s like having 800 filters built in! Spectral scanning capability allows quick determination of peak wavelengths in unknown solutions. - Mutiple Microplate Types
Any standard microplate from 6- to 384-wells can be accommodated (depending on model). In addition, the PowerWave can also read up to 8 of BioTek’s patented Bio-Cell™ quartz vessels for fixed 1 cm measurements. - Choice of Reading Speeds
PowerWave microplate spectrophotometers provides reading speed options based on the assay or system requirements. Normal read speed incorporates BioTek’s enhanced reading capabilities for the best performance, even with high optical density solutions. Rapid speed ensures fast reading at typical optical densities with excellent performance of the result. If faster throughput is required, the PowerWave's Sweep read speed meets the challenge. Using “on the fly” technology, an entire 96-well plate can be read as few as 5 seconds, depending on model. - Temperature Control and Microplate Shaking
BioTek’s 4-Zone™ temperature control is a standard feature with the PowerWave. PowerWave readers use a natural convection system rather than a forced hot air system. This reduces evaporation in the microplate across its broad temperature range of 4° above ambient to 50°C. In the reading chamber, the microplate is completely surrounded by four independently controlled heaters ensuring low variation across the microplate. Users select from four shaking intensities and can define shake time for controlled agitation. - Robotics Compatible
The PowerWave's small footprint makes it ideal for robotic systems. The carrier is designed for easy robot gripper access. PowerWave readers are compatible with both BioTek’s BioStack™ Microplate Stacker and BioStack™ Twister® II Microplate Handler, and can be integrated into many automated systems. - Gen5™ Data Analysis Software included with all models.
Model |
PowerWave HT |
PowerWave 340 |
PowerWave XS2 |
Part # |
RPRWI |
RPRWI340/96 |
MQX200R |
| 96-well only | • | ||
| 96-/384-well | • | ||
| 6- to 384-well | • | ||
| 200-999 nm | • | • | |
| 340-999 nm | • | ||
| Incubation | • | • | • |
| Shaking | • | • | • |
| Take3 Compatible | • | ||
| Software | Gen5™ included with all configurations | ||
| General | |
| Detection method | Absorbance |
| Read method | Endpoint, kinetic, spectral, linear and well area scanning (depending on model) |
| Microplate types | 96- & 384-well (PowerWave HT) 96-well (PowerWave 340) 6- to 384-well (PowerWave XS, XS2) |
| Temperature control | Ambient +4°C to 50°C 4-Zone natural convection |
| Shaking | User-programmable timing and frequency |
| Software | Includes Gen5 Software |
| Automation | Compatible with BioStack Microplate Stacker and BioStack Twister II Microplate Handler. |
| Absorbance | |
| Light source | Xenon Flash lamp |
| Wavelength selection | Monochromator, selectable 1 nm or greater increments |
| Wavelength range | 200 to 999 nm 340 to 999 nm (PowerWave 340) |
| Bandpass | 5 nm (PowerWave 340 and PowerWave HT) 2.4 nm (PowerWave XS, XS2) |
| Dynamic range | 0 to 4.0 OD |
| Resolution | 0.001 OD (0.0001 OD - PowerWave XS2) |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | 1% + 0.01 OD typical |
| OD linearity | +1% typical |
| OD repeatability | 0.5% + 0.005 OD typical |
| Stray light | 0.03% at 230 nm typical |
| Reading speed | PowerWave 340 and PowerWave HT:
|
| Physical Characteristics | |
| Power | 100 VA max; Instrument powered by universal input (100-240 VAC 50-60 Hz) power supply. |
| Dimensions | 16"D x 8.5"W x 8.5"H (40.6 x 21.6 x 21.6 cm) |
| Weight | 24 lbs (11 kg) |
| Regulatory | |
| Regulatory | For in vitro diagnostic use. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek. |
Specifications are subject to change.
Standard (Included)

Gen5 Data Analysis Software
Optional

BioStack Microplate Stacker

BioStack Twister II Microplate Handler
Standard (Included) | ||
Cables | ||
| 75053 | Serial Cable, Null Modem (9-pin female to 25-pin female) |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
Optional | ||
Cables | ||
| 75104 | USB to Serial Adapter |
|
Labware Holders | ||
| 7302030 | Cuvette Adapter |
|
Miscellaneous | ||
| 7270512 | BioCell Adapter Plate Assembly |
|
| 7272051 | BioCell 1 cm Quartz Vessel |
|
Product Qualification Packages | ||
| 7280520 | PowerWave Qualification Package | |
| 7300514 | PowerWave XS2 Qualification Package | |
Take3 Multi-Volume Plate | ||
| TAKE3 | Take3 Multi-Volume Plate |
|
Test Plates & Reagents | ||
| 7120779 | BioTek QC Check Solution #1 - Yellow (25 mL) |
|
| 7120782 | BioTek QC Check Solution #1 - Yellow (125 mL) |
|
| 7260522 | Absorbance Test Plate |
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