BioTek: Microplate Instrumentation & Software Solutions
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PowerWave Microplate Spectrophotometer

Read 2µL samples!
BioTek’s PowerWave microplate spectrophotometers are designed for use in a myriad of applications, from direct quantitation of nucleic acids using automated pathlength correction to full-scale high throughput operations that demand speed, accuracy and reliability. With rugged hardware, proven optical performance and a very small footprint, PowerWave readers are uniquely designed to provide the most application flexibility while saving space both on the benchtop and in automated systems. Gen5 Data Analysis software comes with PowerWave readers and provides unsurpassed power and versatility.

Use PowerWave plate readers for applications requiring low UV measurements, such as direct DNA quantitation and purity testing, RNA quantitation, as well as visible range measurements for basic ELISA’s, enzyme kinetics, colorimetric assays, among many other applications.



US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

  • No Interference Filters Required
    Monochromator optical design provides continuous wavelength selection from 200 nm to 999 nm (or 340 nm to 999 nm, depending on model). Measuring wavelengths can be selected in 1 nm increments to meet all assay requirements – it’s like having 800 filters built in! Spectral scanning capability allows quick determination of peak wavelengths in unknown solutions.
  • Mutiple Microplate Types
    Any standard microplate from 6- to 384-wells can be accommodated (depending on model). In addition, the PowerWave can also read up to 8 of BioTek’s patented Bio-Cell™ quartz vessels for fixed 1 cm measurements.
  • Choice of Reading Speeds
    PowerWave microplate spectrophotometers provides reading speed options based on the assay or system requirements. Normal read speed incorporates BioTek’s enhanced reading capabilities for the best performance, even with high optical density solutions. Rapid speed ensures fast reading at typical optical densities with excellent performance of the result. If faster throughput is required, the PowerWave's Sweep read speed meets the challenge. Using “on the fly” technology, an entire 96-well plate can be read as few as 5 seconds, depending on model.
  • Temperature Control and Microplate Shaking
    BioTek’s 4-Zone™ temperature control is a standard feature with the PowerWave. PowerWave readers use a natural convection system rather than a forced hot air system. This reduces evaporation in the microplate across its broad temperature range of 4° above ambient to 50°C. In the reading chamber, the microplate is completely surrounded by four independently controlled heaters ensuring low variation across the microplate. Users select from four shaking intensities and can define shake time for controlled agitation.
  • Robotics Compatible
    The PowerWave's small footprint makes it ideal for robotic systems. The carrier is designed for easy robot gripper access. PowerWave readers are compatible with both BioTek’s BioStack™ Microplate Stacker and BioStack™ Twister® II Microplate Handler, and can be integrated into many automated systems.
  • Gen5™ Data Analysis Software included with all models.

 Model

PowerWave HT

PowerWave 340

PowerWave XS2

 Part #

RPRWI

RPRWI340/96

MQX200R

96-well only    
96-/384-well    
6- to 384-well    
200-999 nm  
340-999 nm    
Incubation
Shaking
Take3 Compatible    
Software Gen5™ included with all configurations

 

General
Detection method Absorbance
Read method Endpoint, kinetic, spectral, linear and well area scanning (depending on model)
Microplate types 96- & 384-well (PowerWave HT)
96-well (PowerWave 340)
6- to 384-well (PowerWave XS, XS2)
Temperature control Ambient +4°C to 50°C
4-Zone natural convection
Shaking User-programmable timing and frequency
Software Includes Gen5 Software
Automation Compatible with BioStack Microplate Stacker and BioStack Twister II Microplate Handler.
Absorbance
Light source Xenon Flash lamp
Wavelength selection Monochromator, selectable 1 nm or greater increments
Wavelength range 200 to 999 nm
340 to 999 nm (PowerWave 340)
Bandpass 5 nm (PowerWave 340 and PowerWave HT)
2.4 nm (PowerWave XS, XS2)
Dynamic range 0 to 4.0 OD
Resolution 0.001 OD (0.0001 OD - PowerWave XS2)
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy 1% + 0.01 OD typical
OD linearity +1% typical
OD repeatability 0.5% + 0.005 OD typical
Stray light 0.03% at 230 nm typical
Reading speed PowerWave 340 and PowerWave HT:
  • Normal: 96 wells = 20 sec, 384 = 66 sec
  • Rapid: 96 wells = 11 sec, 384 = 23 sec
  • Sweep: 96 wells = 5 sec, 384 = 11 sec
PowerWave XS, XS2:
  • Normal: 96 wells = 49sec, 384 = 150 sec
  • Rapid: 96 wells = 40 sec, 384 = 112 sec
  • Sweep: 96 wells = 14 sec, 384 = 26 sec
Physical Characteristics
Power 100 VA max; Instrument powered by universal input (100-240 VAC 50-60 Hz) power supply.
Dimensions 16"D x 8.5"W x 8.5"H (40.6 x 21.6 x 21.6 cm)
Weight 24 lbs (11 kg)
Regulatory
Regulatory For in vitro diagnostic use. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek.

Specifications are subject to change.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

The BioStack™ Microplate Stacker adds stacking capabilities to BioTek's Synergy™, PowerWave™, ELx405™, EL406™, NanoQuot™, MicroFlo™ Select, MicroFill™ and Precision™ product families.


BioStack Twister II Microplate Handler

BioStack Twister II Microplate Handler

The BioStack™ Twister® II is a scalable robotic arm that can be interfaced to one or more BioTek readers, washers and liquid handling systems with incremental storage capabilities and automation for entire work cells.


Standard (Included)

Cables

75053 Serial Cable, Null Modem (9-pin female to 25-pin female) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

Cables

75104 USB to Serial Adapter accessory has picture

Labware Holders

7302030 Cuvette Adapter accessory has picture

Miscellaneous

7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

7280520 PowerWave Qualification Package
7300514 PowerWave XS2 Qualification Package

Take3 Multi-Volume Plate

TAKE3 Take3 Multi-Volume Plate accessory has picture

Test Plates & Reagents

7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7260522 Absorbance Test Plate accessory has picture

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