PowerWave HT Microplate Spectrophotometer

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High-throughput monochromator-based absorbance

BioTek’s PowerWave HT microplate spectrophotometer is designed for use in a myriad of applications, from direct quantitation of nucleic acids using automated pathlength correction to full-scale high throughput operations that demand speed, accuracy and reliability. With rugged hardware, proven optical performance and a very small footprint, the PowerWave  HT is uniquely designed to provide the most application flexibility while saving space both on the benchtop and in automated systems. Gen5 Data Analysis software comes with PowerWave HT, providing unsurpassed power and versatility.

Use PowerWave HT for applications requiring low UV measurements, such as direct DNA quantitation and purity testing, RNA quantitation, as well as basic ELISA’s, enzyme kinetics, colorimetric assays, spectral scanning and many other applications.



US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

  • No Interference Filters Required
    Monochromator optical design provides continuous wavelength selection from 200 nm to 999 nm. Measuring wavelengths can be selected in 1 nm increments to meet all assay requirements – it’s like having 800 filters built in! Spectral scanning capability allows quick determination of peak wavelengths in unknown solutions.
  • Microplate Types
    96 and 384-well plates measured. In addition, the PowerWave HT can also read up to 8 of BioTek’s patented Bio-Cell™ quartz vessels for fixed 1 cm measurements.
  • Fast Reading Speed
    Using “on the fly” technology, an entire 96-well plate can be read as few as 5 seconds.
  • Temperature Control and Microplate Shaking
    BioTek’s 4-Zone™ temperature control is a standard feature with the PowerWave HT. BioTek microplate readers use a natural convection system rather than a forced hot air system. This reduces evaporation in the microplate across its broad temperature range of 4° above ambient to 50°C. In the reading chamber, the microplate is completely surrounded by four independently controlled heaters ensuring low variation across the microplate. Users select from four shaking intensities and can define shake time for controlled agitation.
  • Robotics Compatible
    The PowerWave HT's small footprint makes it ideal for robotic systems. The carrier is designed for easy robot gripper access. PowerWave HT is compatible with BioStack 3 and BioStack.
  • Gen5™ Data Analysis Software included with all models.

RPRWI
PowerWave HT: 200-999 nm, 96- and 384-well plate reading with programmable shaking and temperature control from ambient +4°C to 50°C.

General
Wavelength Selection Monochromator
Detection mode Absorbance
Read method End point, kinetic, spectral, linear scanning
Microplate types 96- & 384-well
Temperature control Ambient +4°C above ambient to 50°C
Shaking Linear
Software Gen5 Data Analysis Software
Automation BioStack and third party automation compatible
Absorbance
Light source Xenon flash lamp
Wavelength selection Monochromator, selectable 1 nm or greater increments
Wavelength range 200 to 999 nm
Bandpass 5 nm
Dynamic range 0 - 4.0 OD
Resolution 0.001 OD
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy 1% + 0.01 OD typical
OD linearity +1% typical
OD repeatability 0.5% + 0.005 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96 well: 5 seconds
384 well: 11 seconds
Physical Characteristics
Connectivity RS232 port
Power 100-240 VAC
50/60 Hz
Dimensions 16"D x 8.5"W x 8.5"H (40.6 x 21.6 x 21.6 cm)
Weight 24 lbs (11 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Standard (Included)

Cables

75053 Serial Cable, Null Modem (9-pin female to 25-pin female) accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310048 BioStack Integration Kit for PowerWave HT and PowerWave XS accessory has picture

Cables

75104 USB to Serial Adapter accessory has picture

Labware Holders

7302030 Cuvette Adapter accessory has picture

Miscellaneous

7210515 3-Instrument Rack – for third party robotic system integration accessory has picture
7270512 BioCell Adapter Plate Assembly accessory has picture
7272051 BioCell 1 cm Quartz Vessel accessory has picture

Product Qualification Packages

7280520 PowerWave Qualification Package
7300514 PowerWave XS2 Qualification Package

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3Trio Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

7120779 BioTek QC Check Solution #1 - Yellow (25 mL) accessory has picture
7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture
7260522E Absorbance Test Plate accessory has picture

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